Caco-2 in vitro model of human gastrointestinal tract for studying the absorption of titanium dioxide and silver nanoparticles from seafood

Prospects of surfactant assisted dispersive liquid–liquid microextraction for the selective extraction of silver and titanium dioxide nanoparticles from tap water and determination by spICP-MS

“…Measurements based on 49 Ti have been found to be sensitive enough when assessing Ti in complex samples. 35–39…”

“…Measurements based on 49 Ti have been found to be sensitive enough when assessing Ti in complex samples. [35][36][37][38][39] A tuning solution containing Be, Ce, Fe, In, Li, Mg, Pb, and U (1.0 mg L −1 ) and Ag or Ti (10 mg L −1 ) was used to adjust the ICP-MS prior to analysis. The ow rate was daily determined by weighing the amount of deionized ultrapure water aspirated by the sample introduction system for 1.0 min (measurements in triplicate).…”

Atomic spectrometry update: review of advances in the analysis of metals, chemicals and materials

“…Due to use of TiO 2 as an additive in foods and consumer products, and therefore possible implications on human health and the environmental impact , there remains interest in the detection of TiO 2 NPs in various sample types. An assessment of the bioaccessibility of TiO 2 NPs and Ag NPs from molluscs has recently been undertaken by Taboada-Lopez et al 275 Initially, the presence of NPs in the molluscs was assessed using sNP-ICP-MS, after an enzymatic extraction with pancreatin and lipase. Subsequently, the wet molluscs were homogenised and suspended in an HCl/pepsin mixture at pH 2, to represent the gastric phase, followed by adjustment of the solution pH to 7 and the addition of pancreatin, bile salts and sodium dihydrogen carbonate, to represent the intestinal phase, with each phase shaken at 37 °C for two hours.…”

Analytical Strategies and Method Validation to Access the Bioavailability of Essential Elements and Arsenic from Raw and Cooked Fish and Shrimp

“…A poor bioavailability (<0.02%) was reported for Ag, whereas absorption of 18−68% was found for Ti. 18 In a previous study, Siedlikowski and co-workers reported bioavailability of approximately 60% for methylmercury (MeHg) from raw tilapia and shrimp samples after bioassays using Caco-2 cells. 19 To the best of our knowledge, the influence of cooking methods on the bioavailability of essential elements and arsenic from these species has not yet been studied.…”

“…Recently, Taboada-López and co-workers used the Caco-2 epithelial barrier model to estimate the absorption of Ti and Ag from mollusks. A poor bioavailability (<0.02%) was reported for Ag, whereas absorption of 18–68% was found for Ti . In a previous study, Siedlikowski and co-workers reported bioavailability of approximately 60% for methylmercury (MeHg) from raw tilapia and shrimp samples after bioassays using Caco-2 cells .…”