Ca2+ roles in electroporation-induced changes of cancer cell physiology: From membrane repair to cell death

Navickaitė, Diana; Ruzgys, Paulius; Maciulevičius, Martynas; Dijk, Gerwin; O’Connor, Rodney; Šatkauskas, Saulius

doi:10.1016/j.bioelechem.2021.107927

Cited by 13 publications

(4 citation statements)

References 64 publications

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“…Electroporation, a commonly used cell transfection technique, employs brief, high-intensity electric fields to enhance the permeability of the cell membrane's phospholipid bilayer [116,117]. It allows the introduction of exogenous molecules, such as nucleic acids and proteins, into the cell [117,118].…”

Section: Electroporationmentioning

confidence: 99%

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Li,

Xue,

et al. 2023

Electrophoresis

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Single‐cell biophysical properties play a crucial role in regulating cellular physiological states and functions, demonstrating significant potential in the fields of life sciences and clinical diagnostics. Therefore, over the last few decades, researchers have developed various detection tools to explore the relationship between the biophysical changes of biological cells and human diseases. With the rapid advancement of modern microfabrication technology, microfluidic devices have quickly emerged as a promising platform for single‐cell analysis offering advantages including high‐throughput, exceptional precision, and ease of manipulation. Consequently, this paper provides an overview of the recent advances in microfluidic analysis and detection systems for single‐cell biophysical properties and their applications in the field of cancer. The working principles and latest research progress of single‐cell biophysical property detection are first analyzed, highlighting the significance of electrical and mechanical properties. The development of data acquisition and processing methods for real‐time, high‐throughput, and practical applications are then discussed. Furthermore, the differences in biophysical properties between tumor and normal cells are outlined, illustrating the potential for utilizing single‐cell biophysical properties for tumor cell identification, classification, and drug response assessment. Lastly, we summarize the limitations of existing microfluidic analysis and detection systems in single‐cell biophysical properties, while also pointing out the prospects and future directions of their applications in cancer diagnosis and treatment.

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Section: Electroporationmentioning

confidence: 99%

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Li,

Xue,

et al. 2023

Electrophoresis

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“…In contrast, the mechanical or non-thermal ablation involves the transfer of acoustic power by HIFU waves, inducing cavitation at the target site and resulting in the mechanical disruption of cell membranes. High-pressure acoustic waves induce changes in the gaseous composition within tissues, instigating oscillation and subsequent rupture of bubbles, causing subcellular-scale mechanical damage to tissues (15, 16).…”

Section: Introductionmentioning

confidence: 99%

Experimental and Computational Analysis of HIFU Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

Badawe,

Harouz,

Abu

et al. 2023

Preprint

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ObjectiveThe primary objective of our study was to investigate the efficiency of high intensity focused ultrasound (HIFU) ablation in two distinct cellular configurations, 2D monolayers and 3D spheroids of epithelial breast cancer cell lines. The study also compares empirical findings from experiments with results obtained through numerical simulations using a bioheat computational model. This comparison is intended to provide a comprehensive understanding of the acoustic energy conversion within the biological system during HIFU treatment.MethodsHIFU was applied to 2D and 3D cultured MDA-MB 231 and MCF7 epithelial breast cancer cell lines while systematically varying ultrasound intensity and duty cycle (DC) during sonication sessions of different durations. Temperature elevation was measured and the ablation percentage was calculated based on bright field and fluorescent imaging of the treated regions. Experimental results were validated through simulations of the ablation setup.ResultsUpon HIFU, spheroids exhibited a lower temperature increase (approximately 20 °C) when subjected to comparable acoustic intensities and duty cycles. The level of tumor ablation was highly influenced by DC, with higher DCs leading to greater ablation percentages. However, sonication duration had a minimal impact on the degree of ablation. Numerical simulations corroborated these observations, demonstrating uniform heat distribution within the cultured cells. At higher DCs and intensities, complete ablation of spheroids was achieved, whereas at lower levels, only the outermost layers exhibited ablation.ConclusionOur study reveals a significant disparity in the response of 2D monolayers and 3D spheroids to HIFU treatment. Specifically, tumor spheroids require lower temperature elevations for effective ablation, and their ablation percentage significantly increases with elevated DC.

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“…[29][30][31] Most all-atom MD models used to study electroporation mechanism were based on pure phospholipid membrane models. [32][33][34] However, the presence of different phospholipid molecules in phospholipid membrane could change its stability and fluidity, which in turn affected the electroporation. [35][36][37] Different phospholipid molecules influenced the shape of the phospholipid membrane and thus regulated protein properties.…”

Section: Introductionmentioning

confidence: 99%

All–atom molecular dynamics simulation of the combined effects of different phospholipids and cholesterol contents on electroporation

Guo

Wang

Zhou

2022

Preprint

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The electroporation mechanism could be related to the composition of the plasma membrane, and the combined effect of different phospholipids molecules and cholesterol contents on electroporation is rarely studied and concluded. In this paper, we applied all-atom molecular dynamics (MD) simulation to study the effects of phospholipids and cholesterol contents on bilayer membrane electroporation. The palmitoyl-oleoyl-phosphatidylcholine (POPC) model, palmitoyl-oleoyl-phosphatidylethanolamine (POPE) model and 1:1 mixed model of the two (PEPC) were three basic models. An electric field of 0.45 V/nm was applied to nine models including three basic models with cholesterol contents of 0\%, 24\%, and 40\%. The interfacial water molecules moved under the electric field, and once the first water bridge formed, the rest of the water molecules would dramatically flood into the membrane. The simulation showed that a rapid rise in the Z component of the average dipole moment of interfacial water (Z-DM) indicated the occurrence of electroporation, and the same increment of Z-DM represented the similar change in the size of water bridge. With the same cholesterol content, the formation of the water bridge was the most rapid in POPC model regarding the average electroporation time ($t_{ep}$), and the average $t_{ep}$ of the PEPC model was close to that of the POPE model. We speculate that the difference in membrane thickness and initial hydrogen bonds of interfacial water affecting the average $t_{ep}$ among different membrane compostion. Our results reveal the influence of membrane composition on electroporation mechanism at the molecular level.

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Ca2+ roles in electroporation-induced changes of cancer cell physiology: From membrane repair to cell death

Cited by 13 publications

References 64 publications

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Experimental and Computational Analysis of HIFU Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids

All–atom molecular dynamics simulation of the combined effects of different phospholipids and cholesterol contents on electroporation

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