Ca2+ mobilization in cumulus cells: Role in oocyte maturation and acrosome reaction

Chen, Hui; Kui, Christopher; Chan, Hsiao Chang

doi:10.1016/j.ceca.2012.11.007

Cited by 11 publications

(4 citation statements)

References 111 publications

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“…The hippo tumor suppressor pathway has functions in follicle cells related tooocyte polarity and development of the body axes in Drosophila [28]. Gonadotropin paracrine factors induce Ca 2+ mobilization in CCs, which affects gamete development and function, and therefore fertilization and reproduction [29]. Furthermore, rat GCs may utilize FSH for biological processes through endocytosis [30].…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression analysis of human cumulus cells

Demiray

Göker

Tavmergen

et al. 2019

Clin Exp Reprod Med

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Objective This study was performed to explore the possibility that each oocyte and its surrounding cumulus cells might have different genetic expression patterns that could affect human reproduction. Methods Differential gene expression analysis was performed for 10 clusters of cumulus cells obtained from 10 cumulus-oocyte complexes from 10 patients. Same procedures related to oocyte maturation, microinjection, and microarray analyses were performed for each group of cumulus cells. Two differential gene expression analyses were performed: one for the outcome of clinical pregnancy and one for the outcome of live birth. Results Significant genes resulting from these analyses were selected and the top 20 affected pathways in each group were analyzed. Circadian entrainment is determined to be the most affected pathway for clinical pregnancy, and proteoglycans in cancer pathway is the most affected pathway for live birth. Circadian entrainment is also amongst the 12 pathways that are found to be in top 20 affected pathways for both outcomes, and has both lowest p -value and highest number of times found count. Conclusion Although further confirmatory studies are necessary, findings of this study suggest that these pathways, especially circadian entrainment in cumulus cells, may be essential for embryo development and pregnancy.

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Section: Discussionmentioning

confidence: 99%

Differential gene expression analysis of human cumulus cells

Demiray

Göker

Tavmergen

et al. 2019

Clin Exp Reprod Med

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“…The movement of sperm towards the oocyte is selected by the function of CCs as a selective barrier. CCs secrete progesterone, an acrosome reaction inducer activated by cell Surface Receptor NYD-SP8-induced Ca 2+ mobilization, and play a role in fertilization by controlling the binding of sperm to the zona pellucida [ 74 ]. Progesterone induces hyperactivated flagellar movement and acrosome reactions, as well [ 75 ].…”

Section: Cumulus Cells With Fertilizationmentioning

confidence: 99%

The Function of Cumulus Cells in Oocyte Growth and Maturation and in Subsequent Ovulation and Fertilization

Turathum

Gao

Chian

2021

Cells

119

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Cumulus cells (CCs) originating from undifferentiated granulosa cells (GCs) differentiate in mural granulosa cells (MGCs) and CCs during antrum formation in the follicle by the distribution of location. CCs are supporting cells of the oocyte that protect the oocyte from the microenvironment, which helps oocyte growth and maturation in the follicles. Bi-directional communications between an oocyte and CCs are necessary for the oocyte for the acquisition of maturation and early embryonic developmental competence following fertilization. Follicle-stimulation hormone (FSH) and luteinizing hormone (LH) surges lead to the synthesis of an extracellular matrix in CCs, and CCs undergo expansion to assist meiotic resumption of the oocyte. The function of CCs is involved in the completion of oocyte meiotic maturation and ovulation, fertilization, and subsequent early embryo development. Therefore, understanding the function of CCs during follicular development may be helpful for predicting oocyte quality and subsequent embryonic development competence, as well as pregnancy outcomes in the field of reproductive medicine and assisted reproductive technology (ART) for infertility treatment.

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“…Cumulus cells perform a number of important functions, including the formation of gap junctions, the transfer of nutrients and hormonal signals to oocytes [39,40], and the alteration of sperm physiology [1,[41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56]. Generally, these functions are described as promoters of fertilization [1,2,[57][58][59]. Through a combination of proteomic, evolutionary, and experimental analyses, we present a complementary hypothesis, that a subset of COC proteins involved in the formation and stabilization of extracellular matrix may have evolved as a mechanism for females to modulate (i.e., slow down) fertilization outcomes.…”

Section: Discussionmentioning

confidence: 99%

Evolutionary, proteomic, and experimental investigations suggest the extracellular matrix of cumulus cells mediates fertilization outcomes†

Keeble

Firman

Sarver

et al. 2021

Biology of Reproduction

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Studies of fertilization biology often focus on sperm and egg interactions. However, before gametes interact, mammalian sperm must pass through the cumulus layer; in mice, this consists of several thousand cells tightly glued together with hyaluronic acid and other proteins. To better understand the role of cumulus cells and their surrounding matrix, we perform proteomic experiments on cumulus oophorus complexes (COCs) in house mice (Mus musculus), producing over 24,000 mass spectra to identify 711 proteins. Seven proteins known to stabilize hyaluronic acid and the extracellular matrix were especially abundant (using spectral counts as an indirect proxy for abundance). Through comparative evolutionary analyses, we show that three of these evolve rapidly, a classic signature of genes that influence fertilization rate. Some of the selected sites overlap regions of the protein known to impact function. In a follow-up experiment, we compared COCs from females raised in two different social environments. Female mice raised in the presence of multiple males produced COCs that were smaller and more resistant to sperm-derived hyaluronidase compared to females raised in the presence of a single male, consistent with a previous study that demonstrated such females produced COCs that were more resistant to fertilization. Although cumulus cells are often thought of as enhancers of fertilization, our evolutionary, proteomic, and experimental investigations implicate their extracellular matrix as a potential mediator of fertilization outcomes.

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Ca2+ mobilization in cumulus cells: Role in oocyte maturation and acrosome reaction

Cited by 11 publications

References 111 publications

Differential gene expression analysis of human cumulus cells

Differential gene expression analysis of human cumulus cells

The Function of Cumulus Cells in Oocyte Growth and Maturation and in Subsequent Ovulation and Fertilization

Evolutionary, proteomic, and experimental investigations suggest the extracellular matrix of cumulus cells mediates fertilization outcomes†

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