Ca2+-Mg2+-dependent ATP-ase activity and calcium homeostasis in children with chronic kidney disease

Polak−Jonkisz, Dorota; Zwołińska, Danuta; Purzyc, Leszek; Musiał, Kinga

doi:10.1007/s00467-006-0329-6

Cited by 9 publications

(5 citation statements)

References 30 publications

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“…All children in our study had features of hyperparathyroidism, which might have been one of the reasons for increased calcium influx into erythrocytes. Increased Ca i 2+ level in erythrocytes may also result from reduced PMCA activity, which was documented for both children and adults with CKD treated conservatively, as well as in adults on renal replacement therapy [5, 6, 10]. Results of the present study are compatible with the above observations.…”

Section: Discussionsupporting

confidence: 91%

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Ca2+-Mg2+-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session

2010

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In the course of chronic kidney disease (CKD) the intracellular erythrocyte calcium (Cai2+) level increases along with the progression of the disease. The decreased activity of Ca2+-Mg2+-dependent ATP-ase (PMCA) and its endogenous modulators calmodulin (CALM), calpain (CANP), and calpastatin (CAST) are all responsible for disturbed calcium metabolism. The aim of the study was to analyze the activity of PMCA, CALM, and the CANP-CAST system in the red blood cells (RBCs) of hemodialyzed (HD) children and to estimate the impact of a single HD session on the aforementioned disturbances. Eighteen patients on maintenance HD and 30 healthy subjects were included in the study. CALM, Cai2+ levels and basal PMCA (bPMCA), PMCA, CANP, and CAST activities were determined in RBCs before HD, after HD, and before the next HD session. Prior to the HD session, the level of Cai2+ and the CAST activity were significantly higher, whereas bPMCA, PMCA, and CANP activities and the CALM level were significantly lower than in controls. After the HD session, the Cai2+ concentration and the CAST activity significantly decreased compared with the basal values, whereas the other parameters significantly increased, although they did not reach the levels of healthy children. The values observed prior to both HD sessions were similar. Cai2+ homeostasis is severely disturbed in HD children, which may be caused by the reduction in the PMCA activity, CALM deficiency, and CANP-CAST system disturbances. A single HD session improved these disturbances but the effect is transient.

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Section: Discussionsupporting

confidence: 91%

“…Our earlier studies have revealed that children and adolescents with CKD stages 2–4 are characterized by a reduced PMCA activity in erythrocytes accompanied by a CALM deficit and disturbances in the CANP-CAST system [5, 6]. The changes result in a high intracellular calcium level.…”

Section: Introductionmentioning

confidence: 99%

Ca2+-Mg2+-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session

2010

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“…[25,26] , by the enhanced calcium influx [9] and the increase in intracellular calcium reserves [10] . The main mechanism of the lymphocyte [Ca 2+ ] i increase involves CRAC channels.…”

Section: Discussionmentioning

confidence: 99%

Purinergic P2X<sub>7</sub> Receptors Participate in Disturbed Intracellular Calcium Homeostasis in Peripheral Blood Mononuclear Cells of Patients with Chronic Kidney Disease

Lajdova

Oksa²,

Chorvát³

et al. 2011

Kidney Blood Press Res

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Background: P2X₇ receptors intervene with lymphocyte activation and are responsible for multiple processes, including calcium influx. Here, we studied the participation of P2X₇ receptors in disturbed intracellular calcium homeostasis regulation in early-stage chronic kidney disease (CKD). Methods: The study involved 20 healthy volunteers and 20 CKD stage 2–3 patients. The free cytosolic calcium concentration ([Ca²⁺]_i) was measured using fluorimetry. The P2X₇ pore function was evaluated by the fluorescent dye ethidium bromide. Results: In peripheral blood mononuclear cells (PBMCs) of patients, [Ca²⁺]_i, intracellular calcium stores and the capacitative calcium entry were increased when compared with healthy subjects. The agonist of P2X₇ receptor BzATP caused a sustained increase in [Ca²⁺]_i in both groups, but the effect was smaller in patients. The antagonist at the P2X₇ receptor KN-62 reduced [Ca²⁺]_i in patients, but had no effect in healthy subjects. In patients, the permeability of ethidium bromide through P2X₇ pores, as well as through BzATP-activated and KN-62-inhibited pores, was distinct from permeability in healthy volunteers. Conclusions: These results demonstrate that the calcium signaling pathway in PBMCs of CKD patients is defective already in CKD stage 2–3, and the pore-forming P2X₇ receptors are involved in these pathophysiological processes.

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“…Massry et al have previously demonstrated that basal levels of [Ca 2þ ] i are greater in several other CKD cell types, including cardiomyocytes [24,25], brain synaptosomes [26], pancreatic islets [27], polymorphonuclear leukocytes [28][29][30], T-cells [31], and platelets [32], and suggested elevated parathyroid hormone (PTH) as a causative factor. Polak-Jonkisz et al [33] identified increased [Ca 2þ ] i in erythrocytes of children with CKD. Lajdova et al [34] isolated human peripheral blood mononuclear cells from 44 adult CKD patients with CKD stage 2-3 and found increased [Ca 2þ ] i in CKD cells compared with normal cells.…”

Section: Introductionmentioning

confidence: 99%

Intracellular calcium increases in vascular smooth muscle cells with progression of chronic kidney disease in a rat model

Rodenbeck

Zarse

McKenney‐Drake

et al. 2016

Nephrol. Dial. Transplant.

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Ca2+-Mg2+-dependent ATP-ase activity and calcium homeostasis in children with chronic kidney disease

Cited by 9 publications

References 30 publications

Ca2+-Mg2+-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session

Ca2+-Mg2+-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session

Purinergic P2X<sub>7</sub> Receptors Participate in Disturbed Intracellular Calcium Homeostasis in Peripheral Blood Mononuclear Cells of Patients with Chronic Kidney Disease

Intracellular calcium increases in vascular smooth muscle cells with progression of chronic kidney disease in a rat model

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