Ca2+Mg2+-atpase activity of synaptosome fraction and synaptosomal membranes from different areas of rat brain

Lachowicz, Lilla; Janiszewska, Grażyna; R, Wojtkowiak; Wojtkowiak, Z

doi:10.1016/0020-711x(83)90061-7

Cited by 6 publications

(1 citation statement)

References 15 publications

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“…There are few studies on the xanthine effects on the ATPase and ecto-nucleotidases. Theophylline caused increase of Ca 2ϩ ,Mg 2ϩ -ATPase activity from crude synaptosomal membranes in hippocampal region (15). Theophylline and caffeine, in concentrations of 1 mM, inhibited Na ϩ ,K ϩ -ATPase (EC 3.6.1.38) activity in rat pancreatic islets (16).…”

Section: Introductionmentioning

confidence: 96%

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Silva

Bruno

Battastini

et al. 2003

Neurochemical Research

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The psychostimulant caffeine promotes behavioral effects such as hyperlocomotion, anxiety, and disruption of sleep by blockade of adenosine receptors. The availability of extracellular adenosine depends on its release by transporters or by the extracellular ATP catabolism performed by the ecto-nucleotidase pathway. This study verified the effect of caffeine on NTPDase 1 (ATP diphosphohydrolase) and 5'-nucleotidase of synaptosomes from hippocampus and striatum of rats. Caffeine and theophylline tested in vitro were unable to modify nucleotide hydrolysis. Caffeine chronically administered in the drinking water at 0.3 g/L or 1 g/L for 14 days failed to affect nucleotide hydrolysis. However, acute administration of caffeine (30 mg/kg, i.p.) produced an enhancement of ATP (50%) and ADP (32%) hydrolysis in synaptosomes of hippocampus and striatum, respectively. This activation of ATP and ADP hydrolysis after acute treatment suggests a compensatory effect to increase adenosine levels and counteract the antagonist action of caffeine.

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Section: Introductionmentioning

confidence: 96%