Ca<sup>2+</sup> signaling differentiation during oocyte maturation

Machaca, Khaled

doi:10.1002/jcp.21194

Cited by 81 publications

(85 citation statements)

References 140 publications

(198 reference statements)

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“…Subsequent studies characterized the fertilization-dependent Ca 2+ release in mammals and other vertebrates and showed specificity in the spatial and temporal pattern of these signals, where in mammals an oscillatory Ca 2+ signal that lasts several hours is observed, whereas in the frog Xenopus laevis for example only a single Ca 2+ transient is induced at fertilization and takes the form of a sweeping Ca 2+ wave [20][21][22]. These differing Ca 2+ dynamics among species are vital in mediating the complex events that need to occur at fertilization to induce the transition from gametogenesis to embryogenesis [20;22;23].…”

Section: Ca 2+ Signals Are Required At Fertilization To Induce Commentioning

confidence: 99%

“…Although not discussed in details here, eggs acquire the ability to produce the specialized Ca 2+ transient at fertilization following a dramatic remodeling of the Ca 2+ signaling machinery during oocyte maturation, which encompasses entry and progression through meiosis [22]. Hence not only are Ca 2+ signals important for cell cycle progression, but also the Ca 2+ signaling machinery remodels during the cell cycle to service the specific needs of the cell during its development [22;23].…”

Section: Ca 2+ Signals Are Required At Fertilization To Induce Commentioning

confidence: 99%

“…IP 3 -dependent Ca 2+ release is sensitized [139][140][141][142] and the plasma membrane Ca 2+ -ATPase (PMCA) is internalized [143;144]. This remodeling allow the egg to produce the fertilization-specific Ca 2+ transient that is essential for egg activation [22].…”

Section: Ca 2+ Signals Induce Cell Cycle Reentry Of Quiescent T-cmentioning

confidence: 99%

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Ca2+ signaling, genes and the cell cycle

Machaca

2011

Cell Calcium

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Changes in the concentration and spatial distribution of Ca 2+ ions in the cytoplasm constitute a ubiquitous intracellular signaling module in cellular physiology. With the advent of Ca 2+ dyes that allow direct visualization of Ca 2+ transients, combined with powerful experimental tools such as electrophysiological recordings, intracellular Ca 2+ transients have been implicated in practically every aspect of cellular physiology, including cellular proliferation. Ca 2+ signals are associated with different phases of the cell cycle and interfering with Ca 2+ signaling or downstream pathways often disrupts progression of the cell cycle. Although there exists a dependence between Ca 2+ signals and the cell cycle the mechanisms involved are not well defined and given the cross-talk between Ca 2+ and other signaling modules, it is difficult to assess the exact role of Ca 2+ signals in cell cycle progression. Two exceptions however, include fertilization and T-cell activation, where well-defined roles for Ca 2+ signals in mediating progression through specific stages of the cell cycle have been clearly established. In the case of T-cell activation Ca 2+ regulates entry into the cell cycle through the induction of gene transcription.

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Section: Ca 2+ Signals Are Required At Fertilization To Induce Commentioning

confidence: 99%

Section: Ca 2+ Signals Are Required At Fertilization To Induce Commentioning

confidence: 99%

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Ca2+ signaling, genes and the cell cycle

Machaca

2011

Cell Calcium

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“…Oocyte maturation is associated with an almost 50% decrease in membrane surface area in preparation for embryonic divisions (11). However, the internalization of membrane proteins is selective as Ca 2ϩ -activated Cl Ϫ channels remain functional on the cell membrane in eggs, whereas other proteins such as the plasma-membrane Ca 2ϩ -ATPase and ␤-integrin are internalized (14). The exocytosis block during maturation, while endocytosis continues, leads to enrichment of channels and transporters within intracellular vesicular compartments, which are eventually inserted into the basolateral membrane of newly forming blastomeres leading to the formation of the first polarized epithelium during embryogenesis (32).…”

Section: Resultsmentioning

confidence: 99%

“…A critical aspect of oocyte maturation is the differentiation of Ca 2ϩ signaling pathways (14). Ca 2ϩ is the universal signal for egg activation in all sexually reproducing species, and fertilization produces a spatially and temporally defined Ca 2ϩ transient that is necessary and sufficient for egg activation (15).…”

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confidence: 99%

Orai1 internalization and STIM1 clustering inhibition modulate SOCE inactivation during meiosis

Sun

Machaca

2009

Proc. Natl. Acad. Sci. U.S.A.

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113

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Store-operated Ca 2؉ entry (SOCE) is a ubiquitous Ca 2؉ influx pathway activated in response to depletion of intracellular Ca 2؉ stores. SOCE is a primary modulator of intracellular Ca 2؉ dynamics, which specify cellular responses. Interestingly, SOCE inactivates during M phase but the mechanisms involved remain unclear. SOCE is mediated by clustering of the ER Ca 2؉ sensor STIM1 in response to Ca 2؉ store depletion, leading to gating of the plasma membrane SOCE channel Orai1. Here we show that SOCE inactivation in meiosis is the result of internalization of Orai1 into an intracellular vesicular compartment and to the inability of STIM1 to cluster in response to store depletion. At rest, Orai1 continuously recycles between the cell membrane and an endosomal compartment. We further show that STIM1-STIM1 interactions are inhibited during meiosis, which appears to mediate the inability of STIM1 to form puncta following store depletion. In contrast, STIM1-Orai1 interactions remain functional during meiosis. Combined, the removal of Orai1 from the cell membrane and STIM1 clustering inhibition effectively uncouple store depletion from SOCE activation in meiosis. Although STIM1 is phosphorylated during meiosis, phosphomimetic and alanine substitution mutations do not modulate STIM1 clustering, arguing that phosphorylation does not mediate STIM1 clustering inhibition during meiosis.phosphorylation ͉ store-operated calcium entry ͉ trafficking

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