Ca2+ and Na+ current patterns during oocyte maturation, fertilization, and early developmental stages of Ciona intestinalis

Cuomo, Annunziata; Silvestre, Francesco; Santis, Rosaria De; Tosti, Elisabetta

doi:10.1002/mrd.20404

Cited by 29 publications

(66 citation statements)

References 79 publications

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“…First, Cuomo et al [2006] showed that immature oocytes underwent a spontaneous GVBD in the presence of calcium. The fact that calcium plays a role in prophase/metaphase transition was also supported by a higher calcium current activity found at the GV stage in comparison to the following meiotic stages where sodium currents start to appear (Fig.…”

Section: Etosti Et Almentioning

confidence: 99%

Ion currents modulating oocyte maturation in animals

Tosti

Boni

Gallo

et al. 2013

Systems Biology in Reproductive Medicine

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Growing oocytes are arrested at the first prophase of meiosis which is morphologically identified by the presence of a large and vesicular nucleus, called the germinal vesicle. The dissolution of the germinal vesicle marks the resumption of meiosis during which the oocyte undergoes massive modifications up to the second meiotic block, which is removed at fertilization. The interval between the first and the second meiotic block is defined as maturation and the events occurring during this period are crucial for ovulation, fertilization, and embryo development. Oocytes are excitable cells that react to stimuli by modifying their electrical properties as a consequence of ion currents flowing through ion channels on the plasma membrane. These electrical changes have been largely described at fertilization whereas little information is available during oocyte maturation. The aim of this review is to give an overview on the involvement of ion channels and ion currents during oocyte maturation in species from invertebrates to mammals. The results summarized here point to the possible functional role of ion channels underlying oocyte growth and maturation.

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Section: Etosti Et Almentioning

confidence: 99%

Ion currents modulating oocyte maturation in animals

Tosti

Boni

Gallo

et al. 2013

Systems Biology in Reproductive Medicine

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“…The role of ion currents in the oocyte physiology is well described. Gametes are cells that during growth, maturation, and activation undergo deep modifications of the plasma membrane electrical properties (Tosti and Boni, 2004;Cuomo et al, 2005Cuomo et al, , 2006Boni et al, 2007). In particular, at fertilization, one of the earliest events is the gating of specific ion channels, which generate a flux of ions across the plasma membrane known as fertilization current (Dale, 1994).…”

Section: Electrical Properties Of Plasma Membranementioning

confidence: 99%

“…The latter Cell death developmental role Trimarchi et al, 2000;Hur et al, 2012 is accompanied by a transient change in the RP, which depolarizes in some aquatic animals (Dale and De Felice, 1984;Chambers, 1989;De Simone et al, 1998;Glahn and Nuccitelli, 2003), and hyperpolarizes in some mammals (Miyazaki, 1988;Gianaroli et al, 1994;Tosti et al, 2002). During early embryogenesis, it has been described as a reduced excitability of the blastomeres (Arnoult and Villaz, 1994;Cuomo et al, 2006). While the canonical K 1 , Na 1 , and Ca 21 ion currents undergo evident modulation during different stages of embryogenesis, the newly formed gap junction channels start to establish intercellular communication between blastomeres to play an essential role in development (Moody et al, 1991).…”

Section: Electrical Properties Of Plasma Membranementioning

confidence: 99%

“…These findings suggested a minor role for Ca 21 and Na 1 currents in the first mitotic embryonic cycle; however, the high membrane permeability corresponded to the initial segregation of different tissues, suggesting a possible correlation between channel expression and the cell lineage establishment post 8-cell stage (Cuomo et al, 2006). This recognized minor role of Na 1 currents notwithstanding, it was shown that inhibition of Na 1 currents during fertilization generates a rosette, i.e., an abnormal embryo previously described in the 1960's (Reverberi and Ortolani, 1962), which is the equivalent of the 8-16 cell stage embryo that has lost its spatial orientation.…”

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confidence: 92%

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Ion currents in embryo development

Tosti

Boni

Gallo

2016

Birth Defects Research Pt C

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Ion channels are proteins expressed in the plasma membrane of electrogenic cells. In the zygote and blastomeres of the developing embryo, electrical modifications result from ion currents that flow through these channels. This phenomenon implies that ion current activity exerts a specific developmental function, and plays a crucial role in signal transduction and the control of embryogenesis, from the early cleavage stages and during growth and development of the embryo. This review describes the involvement of ion currents in early embryo development, from marine invertebrates to human, focusing on the occurrence, modulation, and dynamic role of ion fluxes taking place on the zygote and blastomere plasma membrane, and at the intercellular communication between embryo cell stages.Birth Defects Research (Part C) 108:6-18, 2016.V C 2016 Wiley Periodicals, Inc.

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“…This is an alarming view since, in the past, it was shown a clear role of these currents in driving a correct meiosis resumption and generating normal embryo development [5,6]. On the contrary, each of the three biocides tested appears to affect different reproductive events such as the fertilization current, peculiar electrical event generated in the oocyte by the fertilizing spermatozoon, and the following contraction of the oocyte body.…”

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confidence: 99%

Antifouling Compounds Endangering Marine Invertebrates Reproduction

Aless¹,

Gallo²,

Tosti³

2014

J Marine Sci Res Dev

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EditorialReproduction and embryo development of marine animals occur in the sea water and are processes very sensitive to substances present in the environment that come from industrial and agricultural activities. These compounds are collectively known as xenobiotics due to their extra-environmental origin. Among xenobiotics present in the sea water, a relevant role is played by the anti-fouling compounds. These are used to prevent settlement and growth of organisms on submerged structures of vessels and ships that otherwise should create serious problems of speed slow down.In the 60', chemical industries designed paints based on tin metallic compounds named organotines that included tributyltin (TBT) and triphenyltin (TPT). These biocides demonstrated to be very efficacious in impeding the attachment of marine organisms on the ships surface, however a serious toxic side effect exerted on the wild marine species has emerged during the last decades [1]. Numerous ecotoxicological studies clearly indicated that organotines are highly toxic for sea life since they persist in the water, being recycled from the sediments and entering the food chain. The interference with the endocrine system of marine invertebrates and vertebrates, even at low concentrations, may generate high risk of reproductive disorders up to threaten the survival of the species In particular, the exposure to TBT was clearly associated to the phenomenon of imposex, i.e. an irreversible induction of male sex characteristics on females marine snails [2]. For this reason, the International Maritime Organization Although not all the countries adhered to this ban, chemical industries have started to formulate new friendly antifouling compounds as possible alternatives to organotines. Among them, the organic booster biocides diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) [4] and chlorothalonil are the most widely employed. Since their recent application and release in the environment, no many data are currently available on their toxicity and in particular on their impact on the main parameters and events of reproduction such as gametes physiology, fertilization, and early embryo development of marine species. Our group has performed a comparative investigation on the effects of TBT, diuron and chlorothalonil, as old and new generation antifouling biocides, and to do this it was chosen a very peculiar approach that is the study of the oocyte plasma membrane by looking at the ion currents underlying the reproductive events of the marine invertebrate Ciona intestinalis (ascidians).The use of this model is particularly challenging since ecotoxicological studies used for decades the sea urchin embryo survival to test environmental toxicity, but it was not considered to test also the basic process of fertilization.In our studies, surprisingly we discovered a common action of the three biocides in reducing the sodium currents of the virgin oocytes. This is an alarming view since, in the past, it was shown a clear role of these currents in driving a correct...

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Ca²⁺ and Na⁺ current patterns during oocyte maturation, fertilization, and early developmental stages of Ciona intestinalis

Cited by 29 publications

References 79 publications

Ion currents modulating oocyte maturation in animals

Ion currents modulating oocyte maturation in animals

Ion currents in embryo development

Antifouling Compounds Endangering Marine Invertebrates Reproduction

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