(Ca-40, xn) - (Ca-40, 3p xn)

Lipid Bicelle Micropatterning Using Chemical Lift-Off Lithography

“…It has also been reported that lipid vesicle interaction processes with hydrophobic SAMs can take a long time to reach equilibrium, up to several hours and depend on the specific system (e.g., SAM molecular packing, vesicle preparation, vesicle size), while our incubation protocol occurs on much shorter time scales. [51][52][53] Taken together, the QCM-D data support that both types of lipid nanostructures -bicelles and vesicles -adsorb onto bare and hydrophilic HSC11-OH-functionalized gold surfaces. The bicelle system enables the formation of well-packed adlayers on the hydrophilic SAM region while also inhibiting nonspecific adsorption on the HSC18-functionalized gold surface (hydrophobic SAM region) due to DHPC molecular passivation along with the large contacting surface area of an adsorbing bicelle.…”

“…[2][3][4][5] Early work on SLB micropatterning focused on silica-based surfaces on which solutionphase lipid vesicles typically adsorb and rupture spontaneously to form SLBs. [6][7][8] These strategies dealt with pre-installing material barriers to inhibit vesicle adsorption and/or SLB formation in specific locations on the target surface. 9 For example, Groves et al used photolithographic methods to form grids of photoresist, aluminum oxide, or gold on oxidized silicon substrates where adsorbing vesicles would fuse and rupture on oxidized silicon while the grid regions inhibited vesicle adsorption and/or SLB formation.…”

“…12 Other work has also shown that functionalizing silica-based surfaces with hydrophilic polymers can enable selective SLB patterning across the nano-to micron scales by employing the vesicle fusion method. [13][14][15][16] In marked contrast to silica surfaces, vesicles typically adsorb but do not rupture on gold surfaces, 6,17 thereby limiting opportunities to form micropatterned SLBs based on the aforementioned design principles. Rather, Jenkins et al demonstrated how microcontact printing of hydrophilic self-assembled monolayers (SAMs) on gold surfaces promoted tethered SLB formation from adsorbing vesicles.…”