Ca 2+ and CaM are involved in Al 3+ pretreatment-promoted fluoride accumulation in tea plants ( Camellia sinesis L.)

Zhang, Xianchen; Gao, Hongjian; Wu, Honghong; Yang, Ting; Zhang, Zhengzhu; Mao, Jingdong; Wan, Xiaochun

doi:10.1016/j.plaphy.2015.08.007

Cited by 30 publications

(21 citation statements)

References 42 publications

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“…These pharmacological assays showed that H + ‐ATPase enzyme activity, in the microsomal fraction, was reduced by approximately 80% in response to vanadate, whereas the effect of nitrate and azide was less than 10% (Figure A). These results demonstrated that the purity of our plasma membranes, isolated from the microsomal fraction, was sufficient for further analysis (Zhang et al ).…”

Section: Resultssupporting

confidence: 52%

“…Tea plants at the same stage of growth (seven‐to‐eight leaves) were washed with deionized water and transferred to an artificial climate chamber for one month with a day length of 12 h, temperature of 22 ± 1°C, irradiance of 270 µM m −2 s −1 , and relative humidity of 45% to 50%. Plants were grown for 1 week before treatment in nutrient solution comprised of the following: 1.07 mM (NH 4 ) 2 SO 4 , 0.357 mM Ca(NO 3 ) 2 · 4 H 2 O, 0.1 mM KH 2 PO 4 , 0.4 mM K 2 SO 4 , 0.392 mM CaCl 2 , 1.029 mM MgSO 4 , 6.27 × 10 −3 mM Na‐FeEDTA, 9.25 × 10 −3 mM H 3 BO 3 , 18.2 × 10 −3 mM MnSO 4 · 4H 2 O, 0.39 × 10 −3 mM CuSO 4 , 1.53 × 10 −3 mM ZnSO 4 , and 0.38 mM Na 2 MoO 4 , pH 5.0–5.5 (Zhang et al ). Plants were then grown under NH 4 + ‐nutrient medium (1.427 mM [NH 4 ] 2 SO 4 ) for 8 d. The pH value of the nutrient solution was titrated to 4.0, 5.0, or 6.0, with 0.1 M H 2 SO 4 and NaOH every 8 h. To inhibit nitrification of the NH 4 + in the nutrient solution, the nitrification inhibitor dicyandiamide was added at 1% (w/v) of the N level (Ruan et al ).…”

Section: Methodsmentioning

confidence: 99%

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Efficient iron plaque formation on tea (Camellia sinensis) roots contributes to acidic stress tolerance

Zhang

Chen

et al. 2018

JIPB

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Tea plants grow in acidic soil, but to date, their intrinsic mechanisms of acidic stress tolerance have not been elucidated. Here, we assessed the tea plant response to growth on NH nutrient media having different pH and iron levels. When grown in standard NH nutrient solution (iron insufficient, 0.35 mg L Fe ), tea roots exhibited significantly lower nitrogen accumulation, plasma membrane H -ATPase activity, and protein levels; net H efflux was lower at pH 4.0 and 5.0 than at pH 6.0. Addition of 30 mg L Fe (iron sufficient, mimicking normal soil Fe concentrations) to the NH nutrient solution led to more efficient iron plaque formation on roots and increased root plasma membrane H -ATPase levels and activities at pH 4.0 and 5.0, compared to the pH 6.0 condition. Furthermore, plants grown at pH 4.0 and 5.0, with sufficient iron, exhibited significantly higher nitrogen accumulation than those grown at pH 6.0. Together, these results support the hypothesis that efficient iron plaque formation, on tea roots, is important for acidic stress tolerance. Furthermore, our findings establish that efficient iron plaque formation is linked to increased levels and activities of the tea root plasma membrane H -ATPase, under low pH conditions.

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Section: Resultssupporting

confidence: 52%

Section: Methodsmentioning

confidence: 99%

Efficient iron plaque formation on tea (Camellia sinensis) roots contributes to acidic stress tolerance

Zhang

Chen

et al. 2018

JIPB

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“…Our previous study showed that 1.05 mmol L −1 Al 3+ significantly stimulated Ca 2+ efflux from tea root mature zone cells, ranging from 236.75 to 1334.47 pmol cm −2 s −1 , with a mean value of 700 ± 261 pmol cm −2 s −1 . With the application of Al 3+ plus DIDS, about fivefold higher Ca 2+ efflux was shown in mature zone cells, with the mean value reaching 3690 ± 694 pmol cm −2 s −1 .…”

Section: Resultsmentioning

confidence: 89%

“…Increasing Ca 2+ concentration activated CaM level and participated in ion uptake and transport in plants . Our previous study found that endogenous Ca 2+ and CaM are related to Al 3+ ‐promoted F accumulation in tea plants . Similarly, Al 3+ increased cytoplasmic Ca 2+ concentration and promoted NO 3 − accumulation in maize roots .…”

Section: Discussionmentioning

confidence: 91%

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Al³⁺ -promoted fluoride accumulation in tea plants (Camellia sinensis ) was inhibited by an anion channel inhibitor DIDS

Zhang

Gao

Yang

et al. 2016

J. Sci. Food Agric.

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Fluoride absorption, transportation and tolerance mechanism in Camellia sinensis, and its bioavailability and health risk assessment: a systematic review

Peng

Ren

et al. 2020

J Sci Food Agric

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Tea is the one of the most popular non-alcoholic caffeinated beverages in the world. Tea is produced from the tea plant (Camellia sinensis (L.) O. Kuntze), which is known to accumulate fluoride. This article systematically analyzes the literature concerning fluoride absorption, transportation and fluoride tolerance mechanisms in tea plants. Fluoride bioavailability and exposure levels in tea infusions are also reviewed. The circulation of fluoride within the tea plantation ecosystems is in a positive equilibrium, with greater amounts of fluoride introduced to tea orchards than removed. Water extractable fluoride and magnesium chloride (MgCl 2) extractable fluoride in plantation soil are the main sources of absorption by tea plant root via active transmembrane transport and anion channels. Most fluoride is readily transported through the xylem as F − /F-Al complexes to leaf cell walls and vacuole. The findings indicate that tea plants employ cell wall accumulation, vacuole compartmentalization, and F-Al complexes to co-detoxify fluoride and aluminum, a possible tolerance mechanism through which tea tolerates higher levels of fluoride than most plants. Furthermore, dietary and endogenous factors influence fluoride bioavailability and should be considered when exposure levels of fluoride in commercially available dried tea leaves are interpreted. The relevant current challenges and future perspectives are also discussed.

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Ca 2+ and CaM are involved in Al 3+ pretreatment-promoted fluoride accumulation in tea plants ( Camellia sinesis L.)

Cited by 30 publications

References 42 publications

Efficient iron plaque formation on tea (Camellia sinensis) roots contributes to acidic stress tolerance

Efficient iron plaque formation on tea (Camellia sinensis) roots contributes to acidic stress tolerance

Al³⁺ -promoted fluoride accumulation in tea plants (Camellia sinensis ) was inhibited by an anion channel inhibitor DIDS

Fluoride absorption, transportation and tolerance mechanism in Camellia sinensis, and its bioavailability and health risk assessment: a systematic review

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Ca 2+ and CaM are involved in Al 3+ pretreatment-promoted fluoride accumulation in tea plants ( Camellia sinesis L.)

Cited by 30 publications

References 42 publications

Efficient iron plaque formation on tea (Camellia sinensis) roots contributes to acidic stress tolerance

Efficient iron plaque formation on tea (Camellia sinensis) roots contributes to acidic stress tolerance

Al3+ -promoted fluoride accumulation in tea plants (Camellia sinensis ) was inhibited by an anion channel inhibitor DIDS

Fluoride absorption, transportation and tolerance mechanism in Camellia sinensis, and its bioavailability and health risk assessment: a systematic review

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Al³⁺ -promoted fluoride accumulation in tea plants (Camellia sinensis ) was inhibited by an anion channel inhibitor DIDS