C5 chemotactic fragment induces leukocyte production of tissue factor activity: a link between complement and coagulation.

Muhlfelder, Thomas W.; Niemetz, Julian; Kreutzer, Donald L.; Beebe, D.P.; Ward, P. A.; Rosenfeld, Stephen I.

doi:10.1172/jci109269

Cited by 185 publications

(96 citation statements)

References 16 publications

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“…Because monocytes have shown to be important mediators of fetal damage in this model 9,22 and these cells have been shown to express TF in response to C5a, 23 we investigated whether monocytes contribute to the generation of decidual TF in CBA/J ϫ DBA/2. Monocyte depletion was achieved using Cl 2 MDP clodronate liposomes.…”

Section: Increased Tf Expression On Monocytes From Cba ؋ Dba Matingsmentioning

confidence: 99%

Pravastatin prevents miscarriages in mice: role of tissue factor in placental and fetal injury

Redecha

Rooijen

Torry

et al. 2009

Blood

105

101

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Section: Increased Tf Expression On Monocytes From Cba ؋ Dba Matingsmentioning

confidence: 99%

Pravastatin prevents miscarriages in mice: role of tissue factor in placental and fetal injury

Redecha

Rooijen

Torry

et al. 2009

Blood

105

101

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“…Thus, the association of PMP with endothelial cells (Mause, von Hundelshausen, Zernecke, Koenen, and Weber 2005) may carry activated complement components along the vasculature and accelerate endothelial responses to vascular injury. In addition, C5a is known to induce tissue factor expression by endothelial cells and leukocytes in vitro (Ikeda, Nagasawa, Horiuchi, Nishizaka, and Niho 1997;Muhlfelder, Miemetz, Kreutzer, Beebe, Ward, and Rosenfeld 1979).…”

Section: Pathophysiology Of Platelet Mediated Complement Activationmentioning

confidence: 99%

Platelet Mediated Complement Activation

Peerschke

Ghebrehiwet

2008

Advances in Experimental Medicine and Biology

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“…Fifty microliters of cell suspension containing 0.3-1.8xlO 6 cells was mixed with 50 /il 25 mM CaCl 2 . Clotting was initiated by the addition of 50 fil normal human plasma, and the time for clotting to occur at 37°C was recorded by using a fibrometer (BBL, Cockeysville, Md.).…”

Section: Procoagulant Activity Assaysmentioning

confidence: 99%

Prostacyclin analogues inhibit tissue factor expression in the human monocytic cell line THP-1 via a cyclic AMP-dependent mechanism.

Crutchley

Solomon

Conanan

1992

Arterioscler Thromb

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Increased expression of tissue factor procoagulant by peripheral blood monocytes has been implicated in a number of thrombotic disorders. The present studies were undertaken to determine whether stable analogues of prostacyclin, a potent endothelium-derived platelet inhibitor and vasodilator, could inhibit tissue factor expression by human monocytic cells. Exposure of monocytic tumor THP-1 cells to 100 ng/ml endotoxin, 2 units/ml interleukin-1 beta, or 5 ng/ml tumor necrosis factor-alpha for 4 hours led to increased tissue factor procoagulant activity. Preincubation for 30 minutes with iloprost, ciprostene, and carbacyclin led to a dose-dependent inhibition of tissue factor expression induced by all three challenging agents. Iloprost was the most potent: 50% inhibition occurred at 5 nM, a concentration close to the reported dissociation constant for iloprost binding to the platelet prostacyclin receptor. An orally active analogue, cicaprost, was equally effective against endotoxin-induced tissue factor expression. Carbacyclin and ciprostene were 100 times less potent. Iloprost prevented the endotoxin-induced expression of tissue factor antigen on the surface of THP-1 cells, as determined by flow cytometry. Iloprost (500 pM-50 nM) increased intracellular levels of cyclic AMP. This effect was potentiated by isobutylmethylxanthine, an inhibitor of phosphodiesterase. The inhibitory effects of iloprost on tissue factor expression were also potentiated by isobutylmethylxanthine and mimicked by forskolin and dibutyryl cyclic AMP but not dibutyryl cyclic GMP. These results suggest that prostacyclin may play a role in downregulating tissue factor expression in monocytes, at least in part via elevation of intracellular levels of cyclic AMP.

show abstract

C5 chemotactic fragment induces leukocyte production of tissue factor activity: a link between complement and coagulation.

Cited by 185 publications

References 16 publications

Pravastatin prevents miscarriages in mice: role of tissue factor in placental and fetal injury

Pravastatin prevents miscarriages in mice: role of tissue factor in placental and fetal injury

Platelet Mediated Complement Activation

Prostacyclin analogues inhibit tissue factor expression in the human monocytic cell line THP-1 via a cyclic AMP-dependent mechanism.

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