C3c intrathecal synthesis evaluation in patients with multiple sclerosis

Abstract

-Introduction: Multiple sclerosis (Ms) is a chronic, inflammatory and progressive disease of the central nervous system in which local inflammatory injuries of the brain white matter appears, being the most outstanding feature the myeline loss (demyelination). Objective: to determine if the complement system might be involved in the Ms immunopathogeny favouring the mechanism intervening in the myelin destruction. Method: samples of sera and csF from twelve patients with a diagnosis of Ms obtained at the moment of the admission to the hospital at the beginning of the break out, were collected. Levels of c3c and albumin in sera and in csF were quantified using radial immunodiffusion plates. Results: High values over 80% of intrathecal synthesis were obtained except in one of the patients. Conclusion: Intrathecal synthesis of c3c and its liberation to the csF means that the activation of the complement system in any of the two ways has taken place, and that once performed its biological functions, has suffered a degradation process Key words: cytotoxicity, complement, multiple sclerosis, hypersensibility, immunopathogenicity, reibergram.evaluación de la síntesis intratecal de C3c en pacientes con esclerosis múltiple resuMeN -Introducción: La esclerosis múltiple (eM) es una enfermedad crónica, inflamatoria y progresiva del sistema nervioso central que cursa con la aparición de lesiones inflamatorias focales en la sustancia blanca cerebral, en las que lo más llamativo es la pérdida de mielina (desmielinización). Objetivo: conocer si el sistema de complemento puede estar involucrado en la inmunopatogenia de la eM favoreciendo los mecanismos que median la destrucción de la mielina. Método: se colectaron muestras de suero y Lcr de doce pacientes con diagnóstico de eM obtenidas en el momento del ingreso al inicio del brote. se cuantificaron los niveles de c3c y albúmina en suero y en Lcr en placas de inmunodifusión radial. Resultados: se obtuvieron altos valores que superan el 80% de síntesis intratecal, menos en uno de los pacientes. Conclusion: La síntesis intratecal de c3c y su liberación al Lcr significa que ha sucedido la activación del sistema de complemento en alguna de las dos vías y que una vez cumplidas sus funciones biológicas, ha sufrido un proceso de degradación y liberación al Lcr en forma de c3c.PALABrAs-cLAve: citotoxicidad, complemento, esclerosis múltiple, hipersensibilidad, inmunopatogenia, reibergrama.

Complement Inhibition Alleviates Paraquat-Induced Acute Lung Injury

“…The iC3b is further lysed into cell membrane-attached C3 dg and soluble C3c. Elevated levels of C3c have been found in many clinical inflammatory diseases (24), and are an indicator of complement activation (25). In this study, C3c levels began to increase at 12 hours (P , 0.05), reached a peak at 24 hours, and were sustained for 48 hours after PQ injection (P , 0.01) ( Figure 3M).…”

Treatment With Anti-C5a Antibody Improves the Outcome of H7N9 Virus Infection in African Green Monkeys

“…Immunohistochemistry of the lungs on day 3 also revealed elevated protein expression of C3aR and C5aR in the lung, especially in the bronchiole epithelium and other severely inflamed lung tissues ( Figure 3G, 3H, 3J, and 3K ). The lungs also exhibited increased levels of C3c, which is another indicator of complement activation [18,19] ( Figure 3I and 3L). Thus, the complement system was extensively activated in both the circulation and the lungs after H7N9 infection.…”

Difference in the Source of Anti-AQP4-IgG and Anti-MOG-IgG Antibodies in CSF in Patients With Neuromyelitis Optica Spectrum Disorder

“…A value above this line on the Reibergram indicates the presence of intrathecal IgG synthesis. 25,26 This is useful for estimating the intrathecal synthesis for the total IgG level. After estimating the intrathecal synthesis for each specific antibody, the value of the AI calculated by the following equation can be referenced.…”