C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo

Abstract

Objectives: Lung cancer has been reported as the leading cause of cancer-associated deaths in humans, and its incidence continues to increase in the world. A growing number of studies have shown that dysregulated genes are associated with the occurrence and poor prognosis of a variety of tumors, including non-small cell lung cancer (NSCLC). C1q/tumor necrosis factor-related protein 6 (C1QTNF6), a member of the C1q/tumor necrosis factor-related protein (CTRP) family, has been revealed to play a role in carcinogenesis and cancer progression. Nevertheless, the effects and mechanisms of C1QTNF6 in NSCLC remain unrevealed. Materials and methods: MTT (3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide) and colony formation, flow cytometric and transwell assays were performed to explore the cell function. Real-time PCR (RT-PCR) and Western blot were used to analyze the mRNA and protein expression. Results: In the present study, we found that C1QTNF6 significantly promoted the proliferation of SPCA1 and A549 cells by MTT and colony formation assays. In addition, down-regulation of C1QTNF6 weakened the tumor growth in vivo. Besides, C1QTNF6 remarkably reduced apoptosis by flow cytometric analysis and TUNEL assay. Furthermore, the capability of migration and invasion was obviously enhanced on C1QTNF6 overexpression. Conclusion: Overall, our results demonstrated that inhibition of C1QTNF6 attenuated cell proliferation, migration, invasion and promoted apoptosis in vitro and in vivo of NSCLC. Based on the above results, our study provided us with a new and key perspective in understanding and treating NSCLC.

Angiogenic Factor-Based Signature Predicts Prognosis and Immunotherapy Response in Non-Small-Cell Lung Cancer

“…The results of our study were consistent with those of other past studies. Wei et al ( Zhang and Feng 2021 ) reported that C1QTNF6 was significantly highly expressed in NSCLC tissues and cells and regulated tumor growth, migration, and apoptosis. Similar results have been reported in Japan (Tamotsu et al) ( Takeuchi et al, 2011 ), in which C1QTNF6 has been implicated in tumor angiogenesis in hepatocellular carcinoma.…”

Angiogenic Factor-Based Signature Predicts Prognosis and Immunotherapy Response in Non-Small-Cell Lung Cancer

“…The results of our study were consistent with other past studies. Wei et al [21] reported that C1QTNF6 was signi cantly highly expressed in NSCLC tissues and cells and regulated the tumor growth, migration, and apoptosis. Similar results have been reported in Japan (Tamotsu et al) [22], in which C1QTNF6 has been implicated in tumor angiogenesis with hepatocellular carcinoma.…”

Role of the CTRP family in tumor development and progression (Review)

“…Furthermore, a previous study reported that CTRP6 expression is increased in ccRCC and is positively correlated with disease stage ( 51 ). A very recent study demonstrated that inhibition of CTRP6 can attenuate cell proliferation, migration, invasion and promote apoptosis in vitro and in vivo in non-small cell lung cancer ( 74 ). However, in a previous study, aberrant C1QTNF6 expression is implicated in terrible prognosis accompanied with damage of cell potential in lung adenocarcinoma ( 75 ).…”