C13 Megastigmane Derivatives From Epipremnum pinnatum: β-Damascenone Inhibits the Expression of Pro-Inflammatory Cytokines and Leukocyte Adhesion Molecules as Well as NF-κB Signaling

Pan, San-Po; Pirker, Teresa; Kunert, Olaf; Kretschmer, Nadine; Hummelbrunner, Scarlet; Latkolik, Simone; Rappai, Julia; Dirsch, Verena M.; Bochkov, Valery N.; Bauer, R.

doi:10.3389/fphar.2019.01351

Cited by 9 publications

(4 citation statements)

References 34 publications

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“…NF-κB belongs to a family of transcription factors involved in the regulation of cell differentiation, proliferation PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF-κB signaling pathway and apoptosis. As an inflammatory factor, NF-κB serves an important role in the immune system (11,12). Studies (13)(14)(15) have shown that the NF-κB signaling pathway is activated following MI and promotes ventricular remodeling in rats (16).…”

Section: Introductionmentioning

confidence: 99%

PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF‑κB signaling pathway

Wang

Lin

et al. 2021

Exp Ther Med

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Myocardial infarction (MI), the leading cause of death among patients with cardiovascular diseases, is characterized by acute cardiac muscle injury due to severe impairment of the coronary blood supply, which may lead to cardiogenic shock and cardiac arrest. Particularly interesting new cysteine histidine rich 1 (PINCH1) protein, a key component of the integrin signaling pathway, interacts with several proteins and serves a vital role in numerous cellular processes, including cytoskeleton remodeling, cell proliferation and cell migration. To investigate the role of PINCH1 in heart injury in the present study, PINCH1 was knocked out in the myocardial tissue of mice (age, 18 weeks) to induce MI. In addition, cell viability, migration and apoptosis, as well as the expression levels of NF-κB-associated proteins were determined in murine HL1 cardiomyocytes with a conditional PINCH1 shRNA using Cell Counting Kit-8, Transwell, flow cytometry and western blot assays, respectively. Furthermore, the cardiac expansion and myocardial fibrosis in PINCH1 knockout mice was investigated in vivo by performing morphological and histological examinations. Additionally, the murine ventricular myocardial ultrastructure was evaluated using an electron microscope, and the cardiomyocyte apoptotic rate and expression levels of NF-κB-related proteins were determined using TUNEL and western blot assays, respectively. The results showed that the apoptotic rate in the in vivo PINCH1 knockdown group was significantly increased. In addition, the protein expression levels of NF-κB signaling pathway-related proteins, including NF-κB, myeloid differentiation factor 88, TNF-α and caspase-3, were significantly increased in the in vivo PINCH1 knockdown group compared with the wild-type group, but the protein expression of MMP2 and MMP9 were the opposite. Overall, the in vitro and in vivo results revealed that PINCH1 knockout in mice significantly aggravated MI via the NF-κB signaling pathway.

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Section: Introductionmentioning

confidence: 99%

PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF‑κB signaling pathway

Wang

Lin

et al. 2021

Exp Ther Med

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“…Typically, glycosidic megastigmanes commonly have a 1″ → 6′ linkage in the disaccharide unit. The identification of 3 has been reported in a limited number of plant species [ 38 , 39 , 40 , 41 ] including Epipremnum pinnatum [ 42 ], which, like Colocasia , belongs to the Araceae family.…”

Section: Discussionmentioning

confidence: 99%

Activity of Colocasia esculenta (Taro) Corms against Gastric Adenocarcinoma Cells: Chemical Study and Molecular Characterization

Esposito,

Pisanti,

Mauro

et al. 2023

IJMS

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Colocasia esculenta (L.) Schott is a tuberous plant, also known as taro, employed as food worldwide for its renowned nutritional properties but also traditionally used in several countries for medical purposes. In this study, methanolic extracts were prepared from the corms and leaves of Colocasia, subsequently fractionated via molecular exclusion chromatography (RP-HPLC) and their anti-tumor activity assessed in an in vitro model of gastric adenocarcinoma (AGS cells). Vorm extract and isolated fractions II and III affected AGS cell vitality in a dose-dependent manner through the modulation of key proteins involved in cell proliferation, apoptosis, and cell cycle processes, such as caspase 3, cyclin A, cdk2, IkBα, and ERK. To identify bioactive molecules responsible for anti-tumoral activity fractions II and III were further purified via RP-HPLC and characterized via nuclear magnetic resonance (NMR) and electrospray mass spectrometry (ESI-MS) techniques. The procedure enabled the identification of ten compounds including lignans and neolignans, some isolated for the first time in taro, uncommon megastigmane derivatives, and a gallic acid derivative. However, none of the isolated constituents showed efficacy equivalent to that of the fractions and total extract. This suggests that the whole Colocasia phytocomplex has intriguing anti-tumor activity against gastric cancer.

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“…Due to its low threshold (0.002 μg/L in water) and high odor activity value (OAV), β-damascenone has already been described having as baked/cooked apples, honey, and flowery fruity aromas in previous research ( Mendes-Pinto, 2009 ; Tomasino and Bolman, 2021 ). Furthermore, β-damascenone also exhibits interesting biological activities, including inhibition of the expression of proinflammatory cytokines and leukocyte adhesion molecules and the prevention of sunburn and skin cancer ( Uddin et al, 2012 ; Pan et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Screening of β -damascenone-producing strains in light-flavor Baijiu and its production optimization via response surface methodology

Tang¹,

Lin²,

Jiang³

et al. 2022

Front. Microbiol.

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As a C13-norisoprenoid aroma substance, β-damascenone is a highly important aromatic compound and an active constituent. The purpose of this study was to investigate the change law of β-damascenone during the light-flavor Baijiu brewing process, and screen the indigenous microbial strains that produce this compound and optimize fermentation parameters for improving β-damascenone production using a statistical approach. In this project, Wickerhamomyces anomalus YWB-1 exhibited the highest producing activity of β-damascenone. Fermentation conditions were optimized for β-damascenone production using a one-factor-at-a-time (OFAT) approach. A Plackett-Burman design was subsequently adopted to assess the effects of initial pH, incubation temperature, inoculum size, fermentation period, and original Brix degree. Analysis of variance (ANOVA) showed that the correlation coefficient (R2) of the executive model was 0.9795, and this value was significant (p < 0.05). Three significant variables were optimized at three different coded levels using a Box-Behnken design (BBD) of response surface methodology (RSM). Here, 7.25 μg/L β-damascenone was obtained under the following optimum conditions: initial pH of 3.31, original Brix degree of 10.53%, and fermentation period of 52.13 h. The yield was increased 3.02-fold compared with that obtained under unoptimized conditions. This information is conducive to the control of flavor production by regulating variable parameters in Baijiu fermentation.

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C13 Megastigmane Derivatives From Epipremnum pinnatum: β-Damascenone Inhibits the Expression of Pro-Inflammatory Cytokines and Leukocyte Adhesion Molecules as Well as NF-κB Signaling

Cited by 9 publications

References 34 publications

PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF‑κB signaling pathway

PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF‑κB signaling pathway

Activity of Colocasia esculenta (Taro) Corms against Gastric Adenocarcinoma Cells: Chemical Study and Molecular Characterization

Screening of β -damascenone-producing strains in light-flavor Baijiu and its production optimization via response surface methodology

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