C1 esterase inhibitor ameliorates ischemia reperfusion injury in a swine musculocutaneous flap model

Fries, C. Anton; Villamaria, Carole Y.; Spencer, Jerry R.; Rasmussen, Todd E.; Davis, Michael R.

doi:10.1002/micr.30053

Cited by 13 publications

(6 citation statements)

References 27 publications

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“…34 More recently, C1-inh was shown to be slightly protective of I/R injury in musculocutaneous flaps in a swine model. 10 In our study, the C1-Inh-treated group did not show any significant differences compared with the control group (no ischemia insult); however, results were not as good as with IPC. A mild increase in neovascularization was observed in the C1-Inh group (211.5 AE 51.29 vessels/mm 2 ) compared with the flaps of the I/R group (169.4 AE 30.65 vessels/mm 2 ) but, again, it was not significant.…”

Section: Discussioncontrasting

confidence: 54%

“…9 The role of the complement sys-tem in I/R has been extensively researched in vitro and in vivo; however, there have been few reports of C1-Inh in microsurgery but mild protective effects in ischemic musculocutaneous flaps have been reported. 10 Therefore, the purpose of this study was to evaluate and compare the effects of IPC and C1-Inh administration in an I/R injury skin flap rodent model.…”

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confidence: 99%

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Effects of Ischemic Preconditioning and C1 Esterase Inhibitor Administration following Ischemia-Reperfusion Injury in a Rat Skin Flap Model

Masa

Casado-Pérez

Crespo-Lora

et al. 2020

J Reconstr Microsurg

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Background Ischemia-reperfusion (I/R) injury is a serious condition that can affect the success rate of microsurgical reconstructions of ischemic amputated limbs and complex tissue defects requiring free tissue transfers. The purpose of this study was to evaluate the effects of ischemic preconditioning (IPC) and C1 esterase inhibitor (C1-Inh) intravenous administration following I/R injury in a rat skin flap model. Methods Superficial caudal epigastric skin flaps (3 cm × 7 cm) were performed on 50 Wistar rats that were randomly divided into five groups. Ischemia was not induced in the control group. All other flaps underwent 8 hours of ischemia prior to revascularization: I/R control group (8-hour ischemia), IPC group (preconditioning protocol + 8-hour ischemia), C1-Inh group (8-hour ischemia + C1-Inh), and IPC + C1-Inh group (preconditioning protocol + 8-hour ischemia + C1-Inh). Survival areas were macroscopically assessed after 1 week of surgery, and histopathological and biochemical evaluations were also measured. Results There were no significant differences in flap survival between the treatment groups that were suffering 8 hours of ischemia and the control group. A significant increase in neovascularization and lower edema formation were observed in the IPC group compared with that in the I/R group. Biochemical parameters did not show any significant differences. Conclusion Intravenous administration of C1-Inh did not significantly modulate I/R-related damage in this experimental model, but further research is needed. On the other hand, IPC reduces tissue damage and improves neovascularization, confirming its potential protective effects in skin flaps following I/R injury.

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Section: Discussioncontrasting

confidence: 54%

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confidence: 99%

Effects of Ischemic Preconditioning and C1 Esterase Inhibitor Administration following Ischemia-Reperfusion Injury in a Rat Skin Flap Model

Masa

Casado-Pérez

Crespo-Lora

et al. 2020

J Reconstr Microsurg

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“…Ceruloplasmin is currently considered as one of the main antioxidants of blood plasma [ 28 ]. In addition to the transport of copper, and it neutralizes O2-radicals like superoxide dismutase and binds Fe 2+ and Cu 2+ ions, removing them from the fenton reaction, which is one of the key processes in the initiation of LPO [ 35 ]. In our studies, the values of this indicator for the experimental groups were 113 and 154 mg/l, respectively, with a significant difference between the groups ( p < 0.01).…”

Section: Discussionmentioning

confidence: 99%

Metabolic Profile of Sow Blood Serum after Weaning

Bogolyubova

Rykov

Zaitsev

2022

Veterinary Medicine International

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The aim of our research was to determine the content of protein, carbohydrate, lipid, and mineral metabolites, as well as an antioxidant status of the sow’s blood after weaning and to calculate the correlation between these parameters. The experiment was carried out on twenty clinically healthy crossbred sows (Yorkshire × Landrace). Twenty sows were allocated to one of two groups: (1) 1 day after weaning (group 1, n = 10) and (2) 8 days after weaning (group 2, n = 10). The basis of the sow diet was SK-1 compound feed, balanced in terms of nutrients and energy in accordance with modern standards and the recommended feeding regimen. Sows blood samples were taken and analyzed for the metabolites of nitrogenous, carbohydrate-lipid, and mineral metabolism and indicators of antioxidant status. The results showed that, in group 2, the total protein content was 89.07 g/l, which is 10.2% higher than that in group 1 ( p < 0.05 ); it was mainly achieved due to the globulin fraction. The urea increased by 19.1% ( p < 0.05 ), but the concentrations of magnesium and chlorides decreased by 20.2% ( p < 0.01 ) and 5.43% ( p < 0.05 ), as well as the alkaline phosphatase and ALT activities decreased by 42.5% ( p < 0.05 ). Strong positive correlations of the ceruloplasmin with total protein (0.672) and very strong with globulins (0.780) were observed. There was a strong negative correlation between the AST activity and the TBA-AP content, as well as the values of phospholipids and TAWSA. There are moderate negative correlations of the TBA-AP with magnesium, TAWSA and ALT activity, and moderate positive correlations of the TBA-AP with total protein, albumin, triglycerides, and cholesterol. The revealed tendencies and dependencies will serve as the theoretical basis for the development of practical methods for regulating the level of free-radical reactions.

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“…During last two decades, several research studies attempted attenuation of ischemia-reperfusion injury using different compounds and molecules [21,22,23,24,25,26]. Most of the studies have shown positive or promising results in preclinical settings [21,22,27,28] and some clinical trials are being conducted in this field (clinicaltrials.gov identifiers NCT01905501 and NCT03535623).…”

Section: Introductionmentioning

confidence: 99%

Ischemia-reperfusion injury in a rat microvascular skin free flap model: A histological, genetic, and blood flow study

et al. 2018

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Ischemia reperfusion injury is associated with tissue damage and inflammation, and is one of the main factors causing flap failure in reconstructive microsurgery. Although ischemia-reperfusion (I/R) injury is a well-studied aspect of flap survival, its biological mechanisms remain to be elucidated. To better understand the biological processes of ischemia reperfusion injury, and to develop further therapeutic strategies, the main objective of this study was to identify the gene expression pattern and histological changes in an I/R injury animal model. Fourteen rats (n = 7/group) were randomly divided into control or ischemia-reperfusion group (8 hours of ischemia). Microsurgical anastomoses were objectively assessed using transit-time-ultrasound technology. Seven days after surgery, flap survival was evaluated and tissue samples were harvested for anatomopathological and gene-expression analyses.The I/R injury reduced the survival of free flaps and histological analyses revealed a subcutaneous edema together with an inflammatory infiltrate. Interestingly, the Arginase 1 expression level as well as the ratio of Arginase 1/Nitric oxide synthase 2 showed a significant increase in the I/R group. In summary, here we describe a well-characterized I/R animal model that may serve to evaluate therapeutic agents under reproducible and controlled conditions. Moreover, this model could be especially useful for the evaluation of arginase inhibitors and different compounds of potential interest in reconstructive microsurgery.

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C1 esterase inhibitor ameliorates ischemia reperfusion injury in a swine musculocutaneous flap model

Abstract: C1-inh is protective of IRI and may have utility in free tissue transfer, vascularized composite allotransplantation, and spare parts surgery. © 2016 Crown copyright. Microsurgery © 2016 Wiley Periodicals, Inc. Microsurgery 37:142-147, 2017.

Cited by 13 publications

References 27 publications

Effects of Ischemic Preconditioning and C1 Esterase Inhibitor Administration following Ischemia-Reperfusion Injury in a Rat Skin Flap Model

Effects of Ischemic Preconditioning and C1 Esterase Inhibitor Administration following Ischemia-Reperfusion Injury in a Rat Skin Flap Model

Metabolic Profile of Sow Blood Serum after Weaning

Ischemia-reperfusion injury in a rat microvascular skin free flap model: A histological, genetic, and blood flow study

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