C1-carbon sources for chemical and fuel production by microbial gas fermentation

Dürre, Peter; Eikmanns, Bernhard J.

doi:10.1016/j.copbio.2015.03.008

Cited by 202 publications

(125 citation statements)

References 90 publications

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“…CO dehydrogenase (CODH, E.C. 1.22.99.2), which is a key enzyme in CO metabolism15678, usually catalyzes the oxidation of CO to CO 2 and thus might be applicable for transforming hazardous CO into non-toxic CO 2 in various fields. However, applications for these enzymes have not been intensively explored.…”

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confidence: 99%

Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

Choi

Min

Kim

et al. 2017

Sci Rep

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Although aerobic CO dehydrogenases (CODHs) might be applicable in various fields, their practical applications have been hampered by low activity and no heterologous expression. We, for the first time, could functionally express recombinant PsCODH in E. coli and obtained a highly concentrated recombinant enzyme using an easy and convenient method. Its electron acceptor spectra, optimum conditions (pH 6.5 and 30 °C), and kinetic parameters (kcat of 12.97 s−1, Km of 0.065 mM, and specific activity of 0.86 Umg−1) were examined. Blast furnace gas (BFG) containing 20% CO, which is a waste gas from the steel-making process, was tested as a substrate for PsCODH. Even with BFG, the recombinant PsCODH retained 88.2% and 108.4% activity compared with those of pure CO and 20% CO, respectively. The results provide not only a promising strategy to utilize CO-containing industrial waste gases as cheap, abundant, and renewable resources but also significant information for further studies about cascade reactions producing value-added chemicals via CO2 as an intermediate produced by a CODH-based CO-utilization system, which would ultimately expand the versatility of CODH.

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confidence: 99%

Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

Choi

Min

Kim

et al. 2017

Sci Rep

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“…Not limited only from gasified lignocellulose, industrial waste gas rich of CO, CO2, and H2 or gas waste from municipal solid waste and agriculture and forestry residue can be utilized as the gaseous substrate to produce biofuels and other value added chemicals [1][2][3][4]. The conversion process is able to be undertaken by two routes, thermochemical route by Fischer Trop reaction (metal catalyst base process) and biochemical route through fermentation by employing acetogenic bacteria [1,5].…”

Section: Introductionmentioning

confidence: 99%

Bioethanol Production via Syngas Fermentation

et al. 2018

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Abstract. Bioconversion of C-1 carbon in syngas through microbial fermentation presents a huge potential to be further explored for ethanol production. Syngas can be obtained from the gasification of lignocellulosic biomass, by which most of carbon content of the biomass was converted into CO and CO2. These gases could be further utilized by carbon-fixing microorganism such as Clostridium sp. to produce ethanol as the end product. In order to obtain an optimum process, a robust and high performance strain is required and thus high ethanol yield as the main product can be expected. In this study, series of batch fermentation was carried out to select high performance strains for ethanol production. Bottle serum fermentations were performed using CO-gas as the sole carbon source to evaluate the potential of some Clostridia species such as Clostridium ljungdahlii, C. ragsdalei, and C. carboxidovorans in producing ethanol at various concentration of yeast extract as the organic nitrogen source, salt concentration, and buffer composition. Strain with the highest ethanol production in the optimum media will be further utilized in the upscale fermentation.

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“…industrial waste gases, syngas (CO/CO 2 and H 2 ) and methane (Bertsch and Müller, 2015;Daniell et al, 2012;Dürre and Eikmanns, 2015;Köpke et al, 2011;Latif et al, 2014;Liew et al, 2016b). Production of fuels and chemicals via gas fermentation does not compete for arable land and food resources, in contrast to using farmed sugars as feedstock.…”

Section: Introductionmentioning

confidence: 99%

Arginine deiminase pathway provides ATP and boosts growth of the gas-fermenting acetogen Clostridium autoethanogenum

Valgepea

Loi

Behrendorff

et al. 2017

Metabolic Engineering

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Arginine deiminase pathway provides ATP and boosts growth of the gas-fermenting acetogen Clostridium autoethanogenum Metabolic Engineering, http://dx.doi.org/10.1016Engineering, http://dx.doi.org/10. /j.ymben.2017 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractAcetogens are attractive organisms for the production of chemicals and fuels from inexpensive and non-food feedstocks such as syngas (CO, CO 2 and H 2 ). Expanding their product spectrum beyond native compounds is dictated by energetics, particularly ATP availability. Acetogens have evolved sophisticated strategies to conserve energy from reduction potential differences between major redox couples, however, this coupling is sensitive to small changes in thermodynamic equilibria. To accelerate the development of strains for energy-intensive products from gases, we used a genome-scale metabolic model (GEM) to explore alternative ATP-generating pathways in the gas-fermenting acetogen 1 Present address: Department of Plant and Environmental Science, University of Copenhagen, Copenhagen, Denmark Clostridium autoethanogenum. Shadow price analysis revealed a preference of C.autoethanogenum for nine amino acids. This prediction was experimentally confirmed under heterotrophic conditions. Subsequent in silico simulations identified arginine (ARG) as a key enhancer for growth. Predictions were experimentally validated, and faster growth was measured in media containing ARG (t D~4 h) compared to growth on yeast extract (t D~9 h). The growth-boosting effect of ARG was confirmed during autotrophic growth.Metabolic modelling and experiments showed that acetate production is nearly abolished and fast growth is realised by a three-fold increase in ATP production through the arginine deiminase (ADI) pathway. The involvement of the ADI pathway was confirmed by metabolomics and RNA-sequencing which revealed a ~500-fold up-regulation of the ADI pathway with an unexpected down-regulation of the Wood-Ljungdahl pathway. The data presented here offer a potential route for supplying cells with ATP, while demonstrating the usefulness of metabolic modelling for the discovery of native pathways for stimulating growth or enhancing energy availability.

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C1-carbon sources for chemical and fuel production by microbial gas fermentation

Cited by 202 publications

References 90 publications

Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

Bioethanol Production via Syngas Fermentation

Arginine deiminase pathway provides ATP and boosts growth of the gas-fermenting acetogen Clostridium autoethanogenum

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