C-type lectin protein isoforms of Macrovipera lebetina: cDNA cloning and genetic diversity

Phosphodiesterase from Vipera lebetina venom – Structure and characterization

“…The venom contains smaller amounts of phospholipases [4], disintegrins [5], nerve growth factor [6], L-amino acid oxidase [7], Ctype lectin-like proteins [8], VEGF-like protein [9], etc. Nucleases and nucleotidases, ubiquitously present in snake venoms, are minor components of V. lebetina venom.…”

Antitumoral Potential of Tunisian Snake Venoms Secreted Phospholipases A2

“…Besides, they exert a wide range of biological effects, including anticoagulant, platelet aggregation inhibiting [7, 20, 21], bactericidal [22], anti-HIV [23], antimalarial and anti-parasitic [24], antitumor [21, 25, 26], and recently anti-angiogenic effect [27–29]. Due to this functional diversity, these structurally similar proteins aroused the interest of many researchers as molecular models for study of structure-function relationships.…”

“…One of the main experimental strategies used for the study of myotoxic PLA 2 s is the traditional chemical modification of specific aminoacid residues and examination of the consequent effects upon the enzymatic, toxic, and pharmacological activities. Furthermore, some venom sPLA 2 have no catalytic activity while they exert various toxic and pharmacological effects [17, 21, 26]. The absence of direct correlation between catalytic activity and pharmacological effects has led to the hypothesis that specific actions of sPLA 2 are due to the presence of pharmacological sites on the sPLA 2 surface overlapping or distinct from the catalytic site.…”

“…On the other hand, CLPs were first described as modulators of platelet before their antiadhesive activity was highlighted [48–50]. CLPs are thus able to inhibit integrin-dependent proliferation, migration, invasion, and angiogenesis [26, 44, 51, 52]. Sarray and coworkers have isolated lebectin and lebecetin, two C-type lectins, from Macrovipera lebetina snake venom inhibiting   α 5 β 1- and   α v-containing integrins [43, 44].…”

“…They have a molecular weight of 13737.52, 13705.63, and 13626.64 Da, respectively. They contain, respectively, 121, 120, and 122 aminoacids, including 14 cysteines each [21, 26]. The sequences alignment shows similarity as high as 50% (Figure 2).…”