c‐Myc‐mediated SNRPB upregulation functions as an oncogene in hepatocellular carcinoma

Abstract

Dysregulation of genes involved in alternative splicing contributes to hepatocarcinogenesis. SNRPB, a component of spliceosome, is implicated in human cancers, yet its clinical significance and biological function in hepatocellular carcinoma (HCC) remains unknown. Here, we show that SNRPB expression is increased in HCC tissues, compared with the nontumorous tissues, at both messenger RNA and protein levels in two independent cohorts. High expression of SNRPB is significantly associated with higher pathological grade, vascular invasion, serum alpha-fetoprotein level, tumor metastasis, and poor disease-free and overall survivals. Luciferase reporter and chromatin immunoprecipitation assays demonstrate that SNRPB upregulation in HCC is mediated by c-Myc. Positive correlation is found between SNRPB and c-Myc expression in clinical samples. In vitro studies show that ectopic expression of SNRPB promotes HCC cell proliferation and migration, whereas knockdown of SNRPB results in the opposite phenotypes. Collectively, our data suggest SNRPB function as an oncogene and serve as a potential prognostic factor in HCC.

LncRNA MNX1-AS1 promotes progression of intrahepatic cholangiocarcinoma through the MNX1/Hippo axis

“…An important member of the Myc gene family, c-Myc, has been reported that its increased expression was associated with the progression of diverse cancers, including colorectal cancer, prostate cancer, lung cancer, HCC, bladder cancer, and cholangiocarcinoma [46][47][48][49][50][51][52][53][54] . In hepatobiliary cancers, c-Myc mediated small nuclear ribonucleoprotein polypeptides B (SNRPB) upregulation to facilitate the progression of HCC 54 .…”

SNRPB is a mediator for cellular response to cisplatin in non-small-cell lung cancer

“…As a splicing factor, whether SNRPB regulates cisplatin resistance remains unknown. Several recent studies reported that SNRPB is elevated and acts as an oncogene in multiple cancers, such as NSCLC, liver cancer, cervical cancer, glioblastoma [11][12][13][14]. In these cancers, SNRPB exhibits its oncogenic function by alternative splicing the pre-mRNA of downstream targets.…”

Comprehensive Analysis of the Expression and Prognostic Value for Snrp Members in Hepatocellular Carcinoma

“…The increasing evidence showed the indispensable role of the splicing components in the initiation, angiogenesis, apoptosis, and invasion in cancers [7][8][9][10] . Relevant researches [11][12][13][14][15] had reported the difference of expression and clinical value of a single SNRP member in different types of cancers. The function of SNRPB as an oncogene served as a potential prognostic factor in HCC [11] .…”

“…Even, siRNA depletion of SNRPE or SNRPD1, which contributed to cell death by autophagy, led to a marked reduction of cell viability in breast, lung, and melanoma cancer cell lines [15] . In short, current studies [11][12][13][14][15] presented the differential expression of a single SNRP in a tumor, however, few studies concentrate on the expression and prognostic values of the family of Sm core complex (B, D1, D2, D3, E, F and G) in HCC patients.…”

“…Relevant researches [11][12][13][14][15] had reported the difference of expression and clinical value of a single SNRP member in different types of cancers. The function of SNRPB as an oncogene served as a potential prognostic factor in HCC [11] .…”