c-Myc induces changes in higher order rDNA structure on stimulation of quiescent cells

Shiue, Chiou-Nan; Berkson, Rachel; Wright, Anthony P. H.

doi:10.1038/onc.2009.21

Cited by 67 publications

(66 citation statements)

References 35 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, TTF-I has been shown to induce chromatin remodeling and relieve transcriptional repression of rDNA (46,47), whereas recruit- ment of NoRC to the rDNA promoter by TTF-I has been shown to silence rDNA transcription (91). Interestingly, c-Myc activation appears to induce the binding of TTF-I to rDNA, leading to the rDNA chromatin conformation changes that are associated with growth stimulation (92). Therefore, one may speculate that under certain conditions TTF-I may act in a feedback loop to regulate c-Myc transcription by modulating the formation or stability of the c-Myc G-quadruplex.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Nucleolin as a c-myc G-quadruplex-binding Protein

González¹,

Guo

Hurley³

et al. 2009

Journal of Biological Chemistry

277

329

View full text Add to dashboard Cite

myc is a proto-oncogene that plays an important role in the promotion of cellular growth and proliferation. Understanding the regulation of c-myc is important in cancer biology, as it is overexpressed in a wide variety of human cancers, including most gynecological, breast, and colon cancers. We previously demonstrated that a guanine-rich region upstream of the P1 promoter of c-myc that controls 85-90% of the transcriptional activation of this gene can form an intramolecular G-quadruplex (G4) that functions as a transcriptional repressor element. In this study, we used an affinity column to purify proteins that selectively bind to the human c-myc G-quadruplex. We found that nucleolin, a multifunctional phosphoprotein, binds in vitro to the c-myc G-quadruplex structure with high affinity and selectivity when compared with other known quadruplex structures. In addition, we demonstrate that upon binding, nucleolin facilitates the formation and increases the stability of the c-myc G-quadruplex structure. Furthermore, we provide evidence that nucleolin overexpression reduces the activity of a c-myc promoter in plasmid presumably by inducing and stabilizing the formation of the c-myc G-quadruplex. Finally, we show that nucleolin binds to the c-myc promoter in HeLa cells, which indicates that this interaction occurs in vivo. In summary, nucleolin may induce c-myc G4 formation in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Nucleolin as a c-myc G-quadruplex-binding Protein

González¹,

Guo

Hurley³

et al. 2009

Journal of Biological Chemistry

277

329

View full text Add to dashboard Cite

show abstract

“…Although induction of ribosome biogenesis (RiBi) genes is thought to be an ancient function of MYC (Brown et al 2008), direct activation of Pol I transcription by MYC appears to have been acquired relatively late during evolution. Human and rat rDNA both contain multiple E-box sequences, but their number and spacing are not conserved (Arabi et al 2005;Grandori et al 2005;Shiue et al 2009). Furthermore, Drosophila rDNA does not contain canonical E boxes nor could dMyc binding to rDNA be detected (Grewal et al 2005).…”

Section: Effects Of Myc On Transcription By Pol Imentioning

confidence: 99%

“…MYCdependent alterations in higher order chromatin structure may also contribute to activation of rDNA gene expression. On serum stimulation of quiescent rat fibroblasts, MYC is necessary for full Pol I activity and MYC induction not only facilitated binding of Pol I and TBP to rDNA promoter and upstream enhancer sequences, but also resulted in their recruitment to nontranscribed downstream sequences (Shiue et al 2009). In the presence of MYC, serum stimulation rapidly led to the formation of gene loop structures of rDNA that may enhance transcriptional reinitiation by linking promoter and terminator sequences (Shiue et al 2009).…”

Section: Effects Of Myc On Transcription By Pol Imentioning

confidence: 99%

See 1 more Smart Citation

MYC Regulation of Cell Growth through Control of Transcription by RNA Polymerases I and III

Campbell

White

2014

Cold Spring Harbor Perspectives in Medicine

View full text Add to dashboard Cite

MYC's tumorigenic potential involves increased ribosome biogenesis and translational capacity, which supply the cell with protein required for enhanced cell growth and subsequent cell division. In addition to activation of protein-encoding genes transcribed by RNA polymerase II, MYC must stimulate transcription by RNA polymerase I and RNA polymerase III to meet this synthetic demand. In the past decade our knowledge of the mechanisms and importance of MYC regulation of RNA polymerases I and III has flourished. Here we discuss MYC's influence on transcription by these "odd" RNA polymerases and the physiological impact of this regulation is evaluated with relevance to cancer development and treatment.ENHANCED RIBOSOME BIOGENESIS AND PROTEIN SYNTHESIS REQUIRES RNA POLYMERASE I AND III

show abstract

“…Among these signals, Myc protooncogene plays the most important roles at several stages including rRNA transcription (13)(14)(15), rRNA processing (16), and the export of ribosome subunits from the nucleus to the cytosol (17,18). Consistently, Myc transcriptionally induces multiple genes critical for ribosome biogenesis including the genes for ribosomal proteins (19), upstream binding factors (the transcription factors for RNA polymerase I-mediated transcription) (14), and nucleophosmin (a nuclear export chaperone for ribosome) (18,20).…”

mentioning

confidence: 93%

Drosophila Low Temperature Viability Protein 1 (LTV1) Is Required for Ribosome Biogenesis and Cell Growth Downstream of Drosophila Myc (dMyc)

Kim

Jung

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background:To understand the molecular mechanism of cell growth, novel regulators of cell growth should be identified. Results: LTV1 mutation impairs 40S ribosome subunit synthesis, and it blocks cell growth and ribosome biogenesis induced by dMyc. Conclusion: LTV1 is essential for cell growth and ribosome biogenesis downstream of dMyc. Significance: Characterization of LTV1 will be helpful for understanding Myc-induced tumorigenesis.

show abstract

c-Myc induces changes in higher order rDNA structure on stimulation of quiescent cells

Cited by 67 publications

References 35 publications

Identification and Characterization of Nucleolin as a c-myc G-quadruplex-binding Protein

Identification and Characterization of Nucleolin as a c-myc G-quadruplex-binding Protein

MYC Regulation of Cell Growth through Control of Transcription by RNA Polymerases I and III

Drosophila Low Temperature Viability Protein 1 (LTV1) Is Required for Ribosome Biogenesis and Cell Growth Downstream of Drosophila Myc (dMyc)

Contact Info

Product

Resources

About