C-MORE: A high-content single-cell morphology recognition methodology for liquid biopsies toward personalized cardiovascular medicine

Furkel, Jennifer; Knoll, Maximilian; Din, Shabana; Bogert, N.; Seeger, Timon; Frey, Norbert; Abdollahi, Amir; Katus, Hugo A.; Konstandin, Mathias

doi:10.1016/j.xcrm.2021.100436

Cited by 8 publications

(6 citation statements)

References 44 publications

(54 reference statements)

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“…Almost all cells remained viable and exhibited a green color after 5 days of culture. Cell morphology serves as one of the indicators of cellular physiological status and function [ 60 ]. To further examine the impact of the culture process on cell morphology, this study performed nuclear (blue) and actin cytoskeleton (green) staining on the cells.…”

Section: Resultsmentioning

confidence: 99%

Dynamic hydrogel–metal–organic framework system promotes bone regeneration in periodontitis through controlled drug delivery

Luo,

Yang,

et al. 2024

J Nanobiotechnol

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Periodontitis is a prevalent chronic inflammatory disease, which leads to gradual degradation of alveolar bone. The challenges persist in achieving effective alveolar bone repair due to the unique bacterial microenvironment’s impact on immune responses. This study explores a novel approach utilizing Metal–Organic Frameworks (MOFs) (comprising magnesium and gallic acid) for promoting bone regeneration in periodontitis, which focuses on the physiological roles of magnesium ions in bone repair and gallic acid's antioxidant and immunomodulatory properties. However, the dynamic oral environment and irregular periodontal pockets pose challenges for sustained drug delivery. A smart responsive hydrogel system, integrating Carboxymethyl Chitosan (CMCS), Dextran (DEX) and 4-formylphenylboronic acid (4-FPBA) was designed to address this problem. The injectable self-healing hydrogel forms a dual-crosslinked network, incorporating the MOF and rendering its on-demand release sensitive to reactive oxygen species (ROS) levels and pH levels of periodontitis. We seek to analyze the hydrogel’s synergistic effects with MOFs in antibacterial functions, immunomodulation and promotion of bone regeneration in periodontitis. In vivo and in vitro experiment validated the system's efficacy in inhibiting inflammation-related genes and proteins expression to foster periodontal bone regeneration. This dynamic hydrogel system with MOFs, shows promise as a potential therapeutic avenue for addressing the challenges in bone regeneration in periodontitis. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Dynamic hydrogel–metal–organic framework system promotes bone regeneration in periodontitis through controlled drug delivery

Luo,

Yang,

et al. 2024

J Nanobiotechnol

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“…Cell morphology is a crucial parameter for evaluating the health status of cells, as it is closely related to embryonic development, wound healing, and the process of tumor metastasis [ 29 ]. Regularly inspecting cell morphology during the culture process helps in identifying signs of contamination, nutritional status, and cell degeneration at an early stage.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have highlighted the importance of both tumor cell proliferation and cell mobility in triggering tumor progression [ 29 ]. Therefore, an accurate and real-time assessment of these abilities is crucial for effective tumor control [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Long-term, real-time and label-free live cell image processing and analysis based on a combined algorithm of CellPose and watershed segmentation

Jiang,

Zeng,

et al. 2023

Heliyon

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“…Next, cell growth was analyzed by immunostaining for desmin and measured using the automatic and high throughput IN Cell Analyzer imaging system with the C-MORE software [23]. PE induced cardiomyocyte growth compared to vehicle-treated cells as expected.…”

Section: Resultsmentioning

confidence: 99%

Ythdf2 regulates cardiac remodeling through its m⁶A-mRNA target transcripts

Kmietczyk

Oelschlaeger

Varma

et al. 2022

Preprint

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m6A mRNA methylation controls cardiomyocyte function and increased overall m6A levels are a stereotyping finding in heart failure independent of the underlying etiology. However, it is largely unknown how the information is read by m6A reader proteins in heart failure. Here we show that the m6A reader protein Ythdf2 controls cardiac function and identified a novel mechanism how reader proteins control gene expression and cardiac function. Deletion of Ythdf2 in cardiomyocytes in vivo leads to cardiac hypertrophy, reduced heart function, and increased fibrosis during pressure overload as well as during aging. Similarly, in vitro the knockdown of Ythdf2 results in cardiomyocyte growth and remodeling. Mechanistically, we identified the eucaryotic elongation factor 2 as a major target of Ythdf2 using cell type specific Ribo-seq data. Our study expands our understanding on the regulatory functions of m6A methylation in cardiomyocytes and how cardiac function is controlled by the m6A reader protein Ythdf2.

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C-MORE: A high-content single-cell morphology recognition methodology for liquid biopsies toward personalized cardiovascular medicine

Cited by 8 publications

References 44 publications

Dynamic hydrogel–metal–organic framework system promotes bone regeneration in periodontitis through controlled drug delivery

Dynamic hydrogel–metal–organic framework system promotes bone regeneration in periodontitis through controlled drug delivery

Long-term, real-time and label-free live cell image processing and analysis based on a combined algorithm of CellPose and watershed segmentation

Ythdf2 regulates cardiac remodeling through its m⁶A-mRNA target transcripts

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C-MORE: A high-content single-cell morphology recognition methodology for liquid biopsies toward personalized cardiovascular medicine

Cited by 8 publications

References 44 publications

Dynamic hydrogel–metal–organic framework system promotes bone regeneration in periodontitis through controlled drug delivery

Dynamic hydrogel–metal–organic framework system promotes bone regeneration in periodontitis through controlled drug delivery

Long-term, real-time and label-free live cell image processing and analysis based on a combined algorithm of CellPose and watershed segmentation

Ythdf2 regulates cardiac remodeling through its m6A-mRNA target transcripts

Contact Info

Product

Resources

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Ythdf2 regulates cardiac remodeling through its m⁶A-mRNA target transcripts