“…First, hemolysis assay was performed in reaction mixture under static condition without any shear stress, and is not directly correlated to dynamic erythrocyte filterability. In that filtration process per se induces mechanical stress, the combined mechanical and oxidative stress may accelerate the erythrocyte damage which promotes hemolysis to a greater extent than that observed in the present study 26) . The second limitation is a lack of osmotic control in the reaction mixture of incubation with AAPH.…”