c-Lysozyme from Senegalese sole (Solea senegalensis): cDNA cloning and expression pattern

Molecular characterization and expression pattern of c-type and g-type lysozyme isoforms in starry flounder Platichthys stellate infected with Streptococcus parauberis

“…Within non-challenged control or S. parauberis-challenged group, means with different letters are significantly different at p < 0.05. Asterisks indicate significant differences between control and challenged groups at a given sampling point to stress condition, as suggested by previous studies [1,10]. In contrast, no significant change in mRNA levels in the spleen and kidney was observed for g-type.…”

“…Like its other orthologues, the c-type lysozyme exhibited eight highly conserved cysteine residues that could potentially be involved in forming intramolecular disulfide bridges and be in relation with the enzymatic activity [28]. In addition, the cluster of ten successive residues flanking the active aspartate in the c-type is well conserved, suggesting that c-type lysozyme is functional [10,29]. The presence of a signal peptide in starry flounder c-type lysozyme indicated that it should be an extracellular protein such as other c-type lysozymes.…”

“…Disentangling the genetic basis of innate immunity is of importance in aquacultured organisms, due to the potential applicability of immune-relevant genes as tools for fine understanding and measuring innate immunity [10]. Effectiveness and efficacious functions of immunostimulants and/or vaccines have also been proposed to be related with innate immune parameters of the fishes [17].…”

Genomic characterization, phylogeny and gene regulation of g-type lysozyme in sole (Solea senegalensis)

“…Both lysozymes have displayed distinct antibacterial activity [5e10] and their expression seems to be regulated by bacterial stimulants in fish [5,9e16]. Recently, the c-type lysozyme was cloned and characterized in Senegalese sole [17]. However, little information about regulation and genomic structure of the g-type lysozyme in this species is available.…”

Expression of c-type lysozyme gene in sea cucumber (Apostichopus japonicus) is highly regulated and time dependent after salt stress

“…Comparative Biochemistry and Physiology, Part B 180 (2015) [68][69][70][71][72][73][74][75][76][77][78] A common profile in most of these species-specific studies indicated the c-type is the basal house-keeping lysozyme, while the g-type lysozyme has a more acute role and is thus inducible in the defense against bacterial infections. But, the c-type lysozyme gene is also found expressed in multiple tissues of species and its gene expression can be induced in many cases, but at a much lower level or in other tissues than the g-types, such as in Japanese flounder (Hikima et al, 1997), brill (Jimenez-Cantizano et al, 2008), Senegalese sole (Fernández-Trujilloa et al, 2008), turbot (Zhao et al, 2011), penaeid shrimp (Kaizu et al, 2011), kelp grouper (Harikrishnan et al, 2011), channel catfish (Bilodeau et al, 2006), Atlantic salmon (Myrnes et al, 2013), turbot (Yu et al, 2013), disk abalone (Bathige et al, 2013) and red-spotted grouper (Mai et al, 2014). C-type, g-type and i-type lysozymes are similar in three-dimensional structures but share little homology at primary structure and differ in catalytic mechanism, enzymatic property, and genetic organization (Irwin and Gong, 2003;Callewaert and Michiels, 2010).…”