C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors

He, Di Demi; Tang, Xinyu Thomas; Dong, Wenjie; Cui, Guizhong; Peng, Guangdun; Yin, Xiu-Juan; Chen, Yujie; Jing, Naihe; Zhou, Bo

doi:10.1016/j.stemcr.2020.03.001

Cited by 8 publications

(9 citation statements)

References 45 publications

(57 reference statements)

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“…There exists a difference in the types of MSCs in the embryonic period and after birth. 32 , 33 The results indicate the heterogeneity of MSCs of molars, a finding similar to that reported recently in bone development, which stated that bone formation before and after adolescence is controlled by distinct progenitors. 34 In addition, the heterogeneity of MSCs is related to the location of their origin.…”

Section: Discussionsupporting

confidence: 87%

Tracing PRX1+ cells during molar formation and periodontal ligament reconstruction

Gong

Zhang

et al. 2022

Int J Oral Sci

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Neural crest-derived mesenchymal stem cells (MSCs) are known to play an essential function during tooth and skeletal development. PRX1+ cells constitute an important MSC subtype that is implicated in osteogenesis. However, their potential function in tooth development and regeneration remains elusive. In the present study, we first assessed the cell fate of PRX1+ cells during molar development and periodontal ligament (PDL) formation in mice. Furthermore, single-cell RNA sequencing analysis was performed to study the distribution of PRX1+ cells in PDL cells. The behavior of PRX1+ cells during PDL reconstruction was investigated using an allogeneic transplanted tooth model. Although PRX1+ cells are spatial specific and can differentiate into almost all types of mesenchymal cells in first molars, their distribution in third molars is highly limited. The PDL formation is associated with a high number of PRX1+ cells; during transplanted teeth PDL reconstruction, PRX1+ cells from the recipient alveolar bone participate in angiogenesis as pericytes. Overall, PRX1+ cells are a key subtype of dental MSCs involved in the formation of mouse molar and PDL and participate in angiogenesis as pericytes during PDL reconstruction after tooth transplantation.

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Section: Discussionsupporting

confidence: 87%

Tracing PRX1+ cells during molar formation and periodontal ligament reconstruction

Gong

Zhang

et al. 2022

Int J Oral Sci

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“… 61 , 64 Pdgfrβ-Cre ERT2 Metaphysis, bone marrow, periosteum, a small fraction of growth plate cartilage Perinatal Pdgfrβ + cells are restricted to metaphysis, and juvenile Pdgfrβ + cells are located at metaphysis and bone marrow; Pdgfrβ + cells generate osteoprogenitors, chondrocytes and adipocytes; Pdgfrα + β + metaphyseal SSCs generate diaphyseal SSCs. 137 Gremlin1-Cre ERT Primitive mesenchyme, primary spongiosa at P1, non-perivascular Gremlin1-expressing cells give rise to osteoblasts, chondrocytes and reticular stromal cells, but not adipocytes 97 Kit MerCreMer Fetal chondrocytes, pre-osteoblasts, stromal cells Fetal C-KIT + cells generate ~20% postnatal LepR + cells; Kit MerCreMer does not label postnatal SSCs 118 Cxcl12-Cre ER Perisinusoidal Cxcl12-Cre ER -expressing cells remain quiescent physiologically, and activate to form osteoblasts in response to injury 123 LepR-Cre LepR-Cre ER Perivascular LepR + cells are derived from fetal Col2 + cells; PDGFRα + , Prx1 + , Scf -GFP + , Cxcl12-DsRed high , Nes -GFP low ; highly enriched for CFU-Fs; major source of bone and adipocytes in adult mice; hematopoiesis-supportive 64 , 77 CTSK- mGFP Ctsk-Cre Long bone and calvarial periosteum (endosteal CTSK- mGFP cells are osteoclasts) Periosteal CTSK-mGFP + cells contain TER119 - CD31 − CD45 − THY1.2 − 6C3 − CD200 + CD105 − mSSCs; bone formation via intramembranous ossification physiologically; re-establish endochondral bone formation ability in response to injury 71 Mx1 + αSMA + ( Mx1-Cre;R26-Tdt;αSMA -GFP) Long bone and calvarial periosteum ...…”

Section: Skeletal Stem Cells In Long Bonesmentioning

confidence: 99%

“… 117 One interesting finding is the C-KIT + mesenchymal cells. 118 C-KIT ligand/C-KIT signaling is known to maintain HSCs in bone marrow. Postnatal C-KIT + cells did not mark osteoblasts or adipocytes, but fetal C-KIT + progenitors gave rise to bone and bone marrow stroma.…”

Section: Skeletal Stem Cells In Long Bonesmentioning

confidence: 99%

“…The chondrocytes, pre-osteoblasts and bone marrow stromal cells at E16.5 were labeled when Kit MerCreMer ;R26R- tdTomato mice received tamoxifen at E12.5 and E14.5. 118 However, it is unclear whether the bone marrow C-KIT + cells are derived from perichondrial or growth plate chondrocytes, or re-established in bone marrow.…”

Section: Skeletal Stem Cells In Long Bonesmentioning

confidence: 99%

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Insights into skeletal stem cells

Lei

et al. 2022

Bone Res

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The tissue-resident skeletal stem cells (SSCs), which are self-renewal and multipotent, continuously provide cells (including chondrocytes, bone cells, marrow adipocytes, and stromal cells) for the development and homeostasis of the skeletal system. In recent decade, utilizing fluorescence-activated cell sorting, lineage tracing, and single-cell sequencing, studies have identified various types of SSCs, plotted the lineage commitment trajectory, and partially revealed their properties under physiological and pathological conditions. In this review, we retrospect to SSCs identification and functional studies. We discuss the principles and approaches to identify bona fide SSCs, highlighting pioneering findings that plot the lineage atlas of SSCs. The roles of SSCs and progenitors in long bone, craniofacial tissues, and periosteum are systematically discussed. We further focus on disputes and challenges in SSC research.

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“…In preclinical models, platelet-derived growth factor receptor, but above all c-KIT, two well-known targets of sunitinib, play a key role in bone formation, homeostasis of mesenchymal stem cell, and differentiation into osteogenesis progenitors. We hypothesize that in healing scars, sunitinib exposure modified the mesenchymal stem cell differentiation regulation into osteoblast-like cells [ 9 – 14 ]. Another hypothesis is that sunitinib alters mitochondria functions.…”

Section: Discussionmentioning

confidence: 99%

Myositis ossificans circumscripta after surgery and radiotherapy and during sunitinib treatment: a case report

et al. 2022

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Background Myositis ossificans circumscripta is a self-limiting, benign, ossifying lesion that can affect any type of soft tissue. It is most commonly found in muscles as a solitary lesion. A history of recent trauma has been reported in approximately 50% of cases. Clinically, MOC presents as a painful swelling, which rapidly increases in size. The pain and inflammatory symptoms spontaneously disappear after approximately 2–6 weeks, and the mass stabilizes or decreases. Radiologically, myositis ossificans circumscripta can be divided into two phases. The first is the acute phase, which is followed by the mature phase 2–6 weeks later. During the acute phase, the radiological aspect does not show any specific abnormality. In the mature phase, plain radiographs and computed tomography show blurred calcifications around a hypodense center. We describe here the first case of myositis ossificans circumscripta, with appropriate follow-up, occurring during sunitinib exposure. Case presentation We report a case of myositis ossificans circumscripta in a 34-year-old man (ethnicity unknown) receiving sunitinib for metastatic alveolar soft part sarcoma of the left thigh after surgery and radiotherapy. Four months after the first dose of sunitinib, the patient experienced painful swelling in the surgical scar area. Magnetic resonance imaging showed diffuse and marked edema of the anterior compartment of the thigh, without nodular lesions circumscribing a central core, and without bone signal abnormality. The increased visibility of the intermuscular fascia and convergence of normal muscle fibers (black hole effect), without the displacement seen in tumors, were suggestive of myositis. Therefore, antiangiogenic treatment was discontinued, and the symptoms rapidly resolved within a few days. Three weeks after the discontinuation of sunitinib, the inflammatory findings completely disappeared. Two months after the diagnosis of myositis ossificans circumscripta, plain radiographs and computed tomography showed an extensive calcified mass measuring > 12 cm. The continuation of favorable clinical outcomes was confirmed. Conclusions To the best of our knowledge, this is the first case of myositis ossificans circumscripta with appropriate follow-up occurring during sunitinib exposure. Owing to multimodal treatment of sarcoma, we cannot rule out the radiotherapy and surgery causality.

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C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors

Cited by 8 publications

References 45 publications

Tracing PRX1+ cells during molar formation and periodontal ligament reconstruction

Tracing PRX1+ cells during molar formation and periodontal ligament reconstruction

Insights into skeletal stem cells

Myositis ossificans circumscripta after surgery and radiotherapy and during sunitinib treatment: a case report

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