C-kit (CD117) immunostain is useful for the diagnosis of Giardia lamblia in duodenal biopsies

Sinelnikov, Igor; Sion-Vardy, Netta; Shaco‐Levy, Ruthy

doi:10.1016/j.humpath.2008.07.015

Cited by 43 publications

(8 citation statements)

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“…In 21 patients were seen trophozoites within epithelium, mucosa, lamina propria, submucosa or near of the crypts. The Sinelnikov 37 report is unclear because does not explain in how much of 14 patients they find with intraepithelial trophozoites. Besides, none was able to isolate and culture the Giardia trophozoites.…”

Section: Resultsmentioning

confidence: 96%

“…Reports of intraepithelial Giardia trophozoites are unusual; we found 5 reports in the literature. 33 – 37 In these works, the tissue was processed with standard methods for light or electron microscopy and presents in clear way the trophozoites presence within the duodenal tissue (Table 1 ). Included the patient from this work 64 patients were analyzed.…”

Section: Resultsmentioning

confidence: 99%

“…The literature review showed 20 cases of invasive Giardia trophozoites, including this one there are 21 cases with invasive trophozoites. 33 – 37 Previously we showed trophozoites during the invasion process and goblet cells hyperplasia in samples from dogs. 22 Here, we are showing trophozoites with and without a normal morphology inside the epithelium, many granules surrounded the trophozoites, which may be from eosinophils because EoSVs were recognized.…”

Section: Discussionmentioning

confidence: 99%

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Intraepithelial Giardia Intestinalis

et al. 2014

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The giardiasis is a neglected parasitic disease. The WHO has estimated more than 280 million of human infections each year; however, intraepithelial giardiasis is a rare entity, there are only 5 reports showing invasive giardiasis.A pediatric female patient with chronic abdominal pain, diarrhea, or pasty stools, without fever, was seen in the Gastroenterology and Nutrition Service. The stool studies were negative for pathogens and lactose hydrogen breath test was positive. The presumptive clinical diagnosis was giardiasis and the patient was empirically treated with nitazoxanide. But, the patient persisted with abdominal pain and pasty stools.Endoscopy was indicated to search for Helicobacter and Giardia. Guardian and patient gave written informed consent. Hematological profile was normal. The endoscopy was performed under general anesthesia and the biopsies and duodenal aspirate were obtained. The microscopic analyses of duodenal fluid showed Giardia trophozoites. Electron microscopic analysis was negative for Helicobacter pylori, but Giardia trophozoites with a typical crescent shape within the tissue were found.The patient was treated with tinidazole, subsequent tests showed that lactose absorption was normal, stool examinations were negative for Giardia and abdominal pain had stopped.This case suggest that intraepithelial giardiasis could be a common entity but unseen because the giardiasis diagnosis is usually made on fecal samples. Future studies are necessary to determine the role of intraepithelial trophozoites in giardiasis pathogenic mechanisms

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Intraepithelial Giardia Intestinalis

et al. 2014

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“…Die Diagnose kann durch Stuhlproben (Lichtmikroskopie, ELISA, Floureszenzimmunoassay) oder über eine Duodenalbiopsie bzw. ein Duodenalaspirat erfolgen [6]. Eine antibiotische Therapie ist in der Regel erfolgreich [7].…”

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“…Die Erregerzahl kann hier sehr gering sein, sodass eine immunhistochemische Darstellung für ihre Detektierung nützlich sein kann. Neben spezifischen Antikörpern wurde auch der Einsatz von Antikörper gegen CD 117 beschrieben [6]. Wie häufig sich entzündliche Veränderungen bei der Lambliasis an der Mukosa zeigen, variiert in den Studien.…”

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Lamblieninfektion als Differenzialdiagnose der Zöliakie in der Duodenalbiopsie

Kasper

Kemper

Siebert³

et al. 2010

Z Gastroenterol

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Giardia lamblia is the most common human parasite with a worldwide distribution and fecal-oral way of transmission. Diagnostic procedures include stool examination and gastroduodenoscopy with biopsy or secret aspiration. In most cases histology reveals a dense accumulation of the parasites on the surface of the duodenal mucosa with no or only slight inflammation. In rare cases, a dense inflammatory infiltrate with severe mucosal atrophy and increased count of intraepithelial lymphocytes may be seen. If in such cases the amount of parasites is low, the histological picture may mimic celiac disease. The two presented cases demonstrate the close morphological relationship and show the importance of considering giardiasis in the differential diagnosis in patients with suspected celiac disease.

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Infections of the Gastrointestinal Tract

Bachman

Lamps

2020

Practical Gastrointestinal Pathology

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C-kit (CD117) immunostain is useful for the diagnosis of Giardia lamblia in duodenal biopsies

Cited by 43 publications

References 11 publications

Intraepithelial Giardia Intestinalis

Intraepithelial Giardia Intestinalis

Lamblieninfektion als Differenzialdiagnose der Zöliakie in der Duodenalbiopsie

Infections of the Gastrointestinal Tract

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