C. elegans Slit Acts in Midline, Dorsal-Ventral, and Anterior-Posterior Guidance via the SAX-3/Robo Receptor

Hao, Joe C.; Yu, Timothy W.; Fujisawa, Katsuki; Culotti, Joseph G.; Gengyo-Ando, Keiko; Mitani, Shohei; Moulder, Gary; Barstead, Robert; Tessier‐Lavigne, Marc; Bargmann, Cornelia I.

doi:10.1016/s0896-6273(01)00448-2

Cited by 213 publications

(247 citation statements)

References 49 publications

(3 reference statements)

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“…Furthermore, studies in C. elegans have suggested that Slit repulsion and Netrin attraction act cooperatively during ventral guidance and that DCC/UNC-40 can actually potentiate SAX-3/Robo repulsion independently of Unc-6/Netrin (Hao et al, 2001;Yu et al, 2002). The most likely explanation for the discrepancy between our data and that reported by Bhat is the misidentification of the double-mutant embryos in the earlier study.…”

Section: Independent Functions Of Netrin-fra and Slit-robo Regulate Mcontrasting

confidence: 96%

Independent Functions of Slit–Robo Repulsion and Netrin–Frazzled Attraction Regulate Axon Crossing at the Midline inDrosophila

Garbe¹,

Bashaw²

2007

J. Neurosci.

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Section: Independent Functions Of Netrin-fra and Slit-robo Regulate Mcontrasting

confidence: 96%

Independent Functions of Slit–Robo Repulsion and Netrin–Frazzled Attraction Regulate Axon Crossing at the Midline inDrosophila

Garbe¹,

Bashaw²

2007

J. Neurosci.

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“…The pathfinding of the AVM axon is affected by two parallel guidance cues, UNC-6/Netrin and Slt-1/ Slit. Ventral UNC-6 attracts and dorsal SLT-1 repels the AVM axon ( Figure 7A; Hao et al 2001). The AVM axon grows ventrally at L1 stage.…”

Section: Resultsmentioning

confidence: 93%

Genes Required for Cellular UNC-6/Netrin Localization in Caenorhabditis elegans

et al. 2010

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UNC-6/Netrin is an evolutionarily conserved, secretory axon guidance molecule. In Caenorhabditis elegans, UNC-6 provides positional information to the axons of developing neurons, probably by establishing a concentration gradient from the ventral to the dorsal side of the animal. Although the proper localization of UNC-6 is important for accurate neuronal network formation, little is known about how its localization is regulated. Here, to examine the localization mechanism for UNC-6, we generated C. elegans expressing UNC-6 tagged with the fluorescent protein Venus and identified 13 genes, which are involved in the cellular localization of VenusTUNC-6. For example, in unc-51, unc-14, and unc-104 mutants, the neurons showed an abnormal accumulation of VenusTUNC-6 in the cell body and less than normal level of VenusTUNC-6 in the axon. An aberrant accumulation of VenusTUNC-6 in muscle cells was seen in unc-18 and unc-68 mutants. unc-51, unc-14, and unc-104 mutants also showed defects in the guidance of dorso-ventral axons, suggesting that the abnormal localization of UNC-6 disturbed the positional information it provides. We propose that these genes regulate the process of UNC-6 secretion: expression, maturation, sorting, transport, or exocytosis. Our findings provide novel insight into the localization mechanism of the axon guidance molecule UNC-6/ Netrin.

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“…As in Drosophila and Caenorhabditis elegans (Hao et al, 2001), the function of Slit proteins in the vertebrate nervous system is primarily mediated by Robo receptors (Brose et al, 1999;Li et al, 1999;Yuan et al, 1999;Hutson and Chien, 2002;Long et al, 2004;Sabatier et al, 2004). In the mammalian spinal cord, Robo1, Robo2, and the Robo family protein Rig1/ Robo3 cooperate to mediate the function of Slit proteins in the guidance of commissural axons (Long et al, 2004;Sabatier et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Robo1 and Robo2 Cooperate to Control the Guidance of Major Axonal Tracts in the Mammalian Forebrain

López‐Bendito¹,

Flames²,

Ma³

et al. 2007

J. Neurosci.

201

203

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The function of the nervous system depends on the precision of axon wiring during development. Previous studies have demonstrated that Slits, a family of secreted chemorepellent proteins, are crucial for the proper development of several major forebrain tracts. Mice deficient in Slit2 or, even more so, in both Slit1 and Slit2 have defects in multiple axonal pathways, including corticofugal, thalamocortical, and callosal connections. In the spinal cord, members of the Robo family of proteins help mediate the function of Slits, but the relative contribution of these receptors to the guidance of forebrain projections remains to be determined. In the present study, we addressed the function of Robo1 and Robo2 in the guidance of forebrain projections by analyzing Robo1-, Robo2-, and Robo1;Robo2-deficient mice. Mice deficient in Robo2 and, more dramatically, in both Robo1 and Robo2, display prominent axon guidance errors in the development of corticofugal, thalamocortical, and corticocortical callosal connections. Our results demonstrate that Robo1 and Robo2 mostly cooperate to mediate the function of Slit proteins in guiding the major forebrain projections.

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C. elegans Slit Acts in Midline, Dorsal-Ventral, and Anterior-Posterior Guidance via the SAX-3/Robo Receptor

Cited by 213 publications

References 49 publications

Independent Functions of Slit–Robo Repulsion and Netrin–Frazzled Attraction Regulate Axon Crossing at the Midline inDrosophila

Independent Functions of Slit–Robo Repulsion and Netrin–Frazzled Attraction Regulate Axon Crossing at the Midline inDrosophila

Genes Required for Cellular UNC-6/Netrin Localization in Caenorhabditis elegans

Robo1 and Robo2 Cooperate to Control the Guidance of Major Axonal Tracts in the Mammalian Forebrain

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