C. elegans Notch Signaling Regulates Adult Chemosensory Response and Larval Molting Quiescence

Singh, Komudi; Chao, Michael Y.; Somers, Gerard A.; Komatsu, Hidetoshi; Corkins, Mark E.; Larkins-Ford, Jonah; Tucey, Tim; Dionne, Heather; Walsh, Melissa B.; Beaumont, Emma; Hart, Douglas P.; Lockery, Shawn R.; Hart, Anne C.

doi:10.1016/j.cub.2011.04.010

Cited by 117 publications

(178 citation statements)

References 54 publications

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“…It is also called Pref-1, a factor involved in adipogenesis (20,21,50). Some previous studies had implicated DLK1 and its homologs as positive regulators in LIN-12/ NOTCH signaling (25,51,52), whereas other studies had shown the opposite or no effect of DLK1 on NOTCH signaling (26,27,50,(53)(54)(55)(56). These seemingly contradictory results could be due to different experimental systems used in different studies.…”

Section: Discussionmentioning

confidence: 99%

Maternal and zygotic Zfp57 modulate NOTCH signaling in cardiac development

Shamis

Cullen²,

Liu

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Zfp57 is a maternal–zygotic effect gene that maintains genomic imprinting. Here we report that Zfp57 mutants exhibited a variety of cardiac defects including atrial septal defect (ASD), ventricular septal defect (VSD), thin myocardium, and reduced trabeculation. Zfp57 maternal-zygotic mutant embryos displayed more severe phenotypes with higher penetrance than the zygotic ones. Cardiac progenitor cells exhibited proliferation and differentiation defects in Zfp57 mutants. ZFP57 is a master regulator of genomic imprinting, so the DNA methylation imprint was lost in embryonic heart without ZFP57. Interestingly, the presence of imprinted DLK1, a target of ZFP57, correlated with NOTCH1 activation in cardiac cells. These results suggest that ZFP57 may modulate NOTCH signaling during cardiac development. Indeed, loss of ZFP57 caused loss of NOTCH1 activation in embryonic heart with more severe loss observed in the maternal-zygotic mutant. Maternal and zygotic functions of Zfp57 appear to play redundant roles in NOTCH1 activation and cardiomyocyte differentiation. This serves as an example of a maternal effect that can influence mammalian organ development. It also links genomic imprinting to NOTCH signaling and particular developmental functions.

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Section: Discussionmentioning

confidence: 99%

Maternal and zygotic Zfp57 modulate NOTCH signaling in cardiac development

Shamis

Cullen²,

Liu

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…For example, several lines of evidence implicate Notch signaling in regulating nematode and fly sleep. In worms, perturbing the functions of either Notch receptors (LIN-12 or GLP-1) or ligands (OSM-7 and OSM-11) altered the total quiescence observed during L4 lethargus, albeit in complex fashion (Singh et al 2011). Moreover, decreasing Notch signaling impaired sensory gating, i.e., reduced the arousal threshold of quiescent L4 lethargus larvae.…”

Section: Conservation Of Sleep-related Functions With "Developmental"mentioning

confidence: 99%

Sleep and Development in Genetically Tractable Model Organisms

Kayser

Biron

2016

Genetics

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Sleep is widely recognized as essential, but without a clear singular function. Inadequate sleep impairs cognition, metabolism, immune function, and many other processes. Work in genetic model systems has greatly expanded our understanding of basic sleep neurobiology as well as introduced new concepts for why we sleep. Among these is an idea with its roots in human work nearly 50 years old: sleep in early life is crucial for normal brain maturation. Nearly all known species that sleep do so more while immature, and this increased sleep coincides with a period of exuberant synaptogenesis and massive neural circuit remodeling. Adequate sleep also appears critical for normal neurodevelopmental progression. This article describes recent findings regarding molecular and circuit mechanisms of sleep, with a focus on development and the insights garnered from models amenable to detailed genetic analyses.

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“…The functions of osm-7 and osm-11 in osmotic stress responses are poorly defined, but some insight can be drawn from their recently defined roles in development and behavior (57,106). These genes encode proteins with similarity to ligands for transmembrane Notch receptors, which are internalized and regulate nuclear gene expression after binding to ligands (57,106).…”

Section: R180 Salt and Water Homeostasis In C Elegansmentioning

confidence: 99%

“…These genes encode proteins with similarity to ligands for transmembrane Notch receptors, which are internalized and regulate nuclear gene expression after binding to ligands (57,106). Loss of osm-11 disrupts development of the vulva, the opening of the uterus, and OSM-11 was shown to be a secreted protein that can interact with the C. elegans Notch receptor LIN-12, which regulates specific tissue differentiation events (57).…”

Section: R180 Salt and Water Homeostasis In C Elegansmentioning

confidence: 99%

“…Loss of osm-11 disrupts development of the vulva, the opening of the uterus, and OSM-11 was shown to be a secreted protein that can interact with the C. elegans Notch receptor LIN-12, which regulates specific tissue differentiation events (57). Relative to standard agar, growth of worms on agar containing 400 mM NaCl decreases immunoreactivity for OSM-11 in hypodermal cells, suggesting that decreased expression may occur during exposure to high osmolarity, which would mimic loss-of-function (106). Further work is needed to define signaling downstream from OSM-7 and OSM-11.…”

Section: R180 Salt and Water Homeostasis In C Elegansmentioning

confidence: 99%

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Physiological and molecular mechanisms of salt and water homeostasis in the nematodeCaenorhabditis elegans

Choe

2013

American Journal of Physiology-Regulatory, Integrative and Comp

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Choe KP. Physiological and molecular mechanisms of salt and water homeostasis in the nematode Caenorhabditis elegans. Am J Physiol Regul Integr Comp Physiol 305: R175-R186, 2013. First published June 5, 2013 doi:10.1152/ajpregu.00109.2013.-Intracellular salt and water homeostasis is essential for all cellular life. Extracellular salt and water homeostasis is also important for multicellular organisms. Many fundamental mechanisms of compensation for osmotic perturbations are well defined and conserved. Alternatively, molecular mechanisms of detecting salt and water imbalances and regulating compensatory responses are generally poorly defined for animals. Throughout the last century, researchers studying vertebrates and vertebrate cells made critical contributions to our understanding of osmoregulation, especially mechanisms of salt and water transport and organic osmolyte accumulation. Researchers have more recently started using invertebrate model organisms with defined genomes and well-established methods of genetic manipulation to begin defining the genes and integrated regulatory networks that respond to osmotic stress. The nematode Caenorhabditis elegans is well suited to these studies. Here, I introduce osmoregulatory mechanisms in this model, discuss experimental advantages and limitations, and review important findings. Key discoveries include defining genetic mechanisms of osmolarity sensing in neurons, identifying protein damage as a sensor and principle determinant of hypertonic stress resistance, and identification of a putative sensor for hypertonic stress associated with the extracellular matrix. Many of these processes and pathways are conserved and, therefore, provide new insights into salt and water homeostasis in other animals, including mammals. osmoregulation; cell volume; ion; organic osmolyte; protein homeostasis; model organism SALT AND WATER HOMEOSTASIS is a fundamental requirement for metazoan life. Ion and water transport mechanisms that compensate for changes in composition and volume of intracellular and extracellular compartments have been generally well defined using vertebrate cell and in vivo models (38). Alternatively, the upstream molecular mechanisms that animal cells use to sense deviations in salt and water balance and regulate compensatory responses are still poorly characterized (20). Studies in brewer's yeast demonstrate that osmotic signal detection and transduction within a single eukaryotic cell can be highly complex with numerous components, acting in parallel pathways, that often cross-talk with other processes (40,55). Osmotic signal detection and transduction are likely to be even more complex in metazoans, which require homeostasis of both the extracellular and intracellular compartments and integration of responses between cells. Genetics is an extremely powerful approach for identifying and characterizing genes and proteins that function in complex biological processes but has been underutilized in the field of osmosensing and signal transduction in metazoans. In...

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C. elegans Notch Signaling Regulates Adult Chemosensory Response and Larval Molting Quiescence

Cited by 117 publications

References 54 publications

Maternal and zygotic Zfp57 modulate NOTCH signaling in cardiac development

Maternal and zygotic Zfp57 modulate NOTCH signaling in cardiac development

Sleep and Development in Genetically Tractable Model Organisms

Physiological and molecular mechanisms of salt and water homeostasis in the nematodeCaenorhabditis elegans

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