C. elegans BLMP-1 controls apical epidermal cell morphology by repressing expression of mannosyltransferase bus-8 and molting signal mlt-8

Body stiffness is a mechanical property that facilitates contact-mediated mate recognition in Caenorhabditis elegans

“…In contrast to the lin-4 mutant, lin-28 mutant hermaphrodites retained males precociously at L3 larva stage (Figure 1D). To further corroborate the role of hypodermis, we tested an evolutionarily conserved transcription factor blmp-1 (B lymphocyte-induced maturation protein 1) that controls several aspects of the development of hypodermal cells [39][40][41] . Consistently, loss of blmp-1 in hermaphrodites did not retain males effectively (Figure 1D).…”

“…To find the effectors in the hypodermis, we analyzed published RNA-seq data of blmp-1 mutant hermaphrodites from a previous study 40 . GO analysis of 975 differentially expressed genes revealed an enrichment of cuticular constituents (Figure 1E; Table S1).…”

“…The N2 and blmp-1 transcriptome comparison was performed using the published dataset from Gene Expression Omnibus (Access number: GSE179411) 40 . In brief, differential expression between wild-type and blmp-1 mutant strains was determined with DESeq2 (v1.22.0; padj<0.05 and FDR>2) 66 .…”