C

Prontera, Francesco; Olshausen, Eckart

doi:10.1007/978-3-476-03582-0_3

Mensch Und Landschaft in Der Antike 1999

DOI: 10.1007/978-3-476-03582-0_3

|View full text |Cite

C

Francesco Prontera¹,

Eckart Olshausen²

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2000

2020

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are numerous analytical methods for detecting NT-proBNP, such as enzyme-linked immunosorbent assays (ELISA), 27,28 immunofluorescence, 29,30 and electrochemiluminescence (ECL). [31][32][33][34] These methods typically require some type of immunological or aptameric 35,36 technique to capture the peptide and also a signal amplification mechanism. 37 For example, in 2017, Shi et al developed a sandwich-type (immunoassay) ECL sensor for detecting clinical levels of NT-proBNP, in which the concentration of NT-proBNP in diluted human serum was correlated to the ECL quenching efficiency.…”

mentioning

confidence: 99%

Electrochemical Detection of NT-proBNP Using a Metalloimmunoassay on a Paper Electrode Platform

et al. 2020

View full text Add to dashboard Cite

In this paper, we demonstrate an electrochemical method for detection of the heart failure biomarker, N-terminal prohormone brain natriuretic peptide (NT-proBNP). The approach is based on a paper electrode assembly and a metalloimmunoassay; it is intended for eventual integration into a home-use sensor. Sensing of NT-proBNP relies on the formation of a sandwich immunoassay and electrochemical quantification of silver nanoparticle (AgNP) labels attached to the detection antibodies (Abs). There are four important outcomes reported in this article. First, compared to physisorption of the detection Abs on the AgNP labels, a 27-fold increase in signal is observed when a heterobifunctional cross-linker is used to facilitate this labeling. Second, the assay is selective in that it does not cross-react with other cardiac natriuretic peptides. Third, the assay forms in undiluted human serum (though the electrochemical analysis is carried out in buffer). Finally, and most important, the assay is able to detect NT-proBNP at concentrations between 0.58 and 2.33 nM. This performance approaches the critical NT-proBNP concentration threshold often used by physicians for risk stratification purposes: ~0.116 nM.

show abstract

mentioning

confidence: 99%

Electrochemical Detection of NT-proBNP Using a Metalloimmunoassay on a Paper Electrode Platform

et al. 2020

View full text Add to dashboard Cite

show abstract

Ras protein signalling

Olson

Marais

2000

Seminars in Immunology

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

C

Cited by 2 publications

References 0 publications

Electrochemical Detection of NT-proBNP Using a Metalloimmunoassay on a Paper Electrode Platform

Electrochemical Detection of NT-proBNP Using a Metalloimmunoassay on a Paper Electrode Platform

Ras protein signalling

Contact Info

Product

Resources

About