C-159T Polymorphism of CD14 Gene and Citokine Profile at Atopic Dermatite in Adults

Abstract

Litus O. I., Derkach N. V., Litus V. I. Abstract. The aim of the study was to research polymorphism of the C-159T gene of the CD14 receptor gene and the levels of serum cytokines in exogenous and endogenous atopic dermatitis in adults.Materials and methods. The study included 96 adult patients with atopic dermatitis. The control group consisted of 90 healthy volunteers. The polymerase chain reaction with electrophoretic detection was used to analyze the polymorphism of the gene. The content of total IgE and cytokines of TNF-α, IL-2, γ-IF, IL-4, IL-5, IL-10, TFR-β in serum was determined by ELISA.Results and Discussion. It was found that the prevalence of the CC genotype leads to an increased risk of developing exogenous atopic dermatitis, which is associated with a high level of total IgE, IL-5 and low IL-10, TGF-β at a given genotype. At the same time, the reduction in the risk of development of both endogenous and exogenous atopic dermatitis is associated with prevalence in the population of genotypes CT and TT. Reducing the risk of atopic dermatitis in the presence of the T allele can be explained by the low concentration of IL-5 and high IL-10, TGF-β in comparison with the homozygous genotype of the CC.Conclusions. The risk of development of atopic dermatitis is authentically increased (Р = 0,033) at a prevalence allele with polymorphic site CD14 of a receptor. In the presence of a genotype of CC in patients with exogenous atopic dermatitis high levels of the general IgE, IL-5 and low IL-10, TFR-β in comparison with other genotypes are characteristic.Decreasing the risk of developing exogenous atopic dermatitis endogenous is associated with a prevalence in population of genotypes of СT and TT. The risk of developing atopic dermatitis in the presence allele is reduced. The T is characterized by low concentration of IL-5 and high IL-10, TFR-β in comparison with a homozygous genotype of CC.The risk of atopic dermatitis development is increased with the prevalence of allele C and decreased with allele T of the polymorphic region C-159T of the CD14 receptor.