Bystin-like protein is upregulated in hepatocellular carcinoma and required for nucleologenesis in cancer cell proliferation

Wang, Hanzhi; Xiao, Wei; Zhou, Qinbo; Chen, Yun; Yang, Shuo; Sheng, Jiansong; Yin, Yanqing; Fan, Jian‐Gao

doi:10.1038/cr.2009.99

Cited by 34 publications

(41 citation statements)

References 46 publications

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“…The dysregulation of ribosome biogenesis has shown to be associated with tumor progression of breast adenocarcinoma MCF-7 cell line [27]. The ribosome assembly factor bystin is overexpressed in hepatocellular carcinoma and is required for cell growth and tumor development [28,29]. These findings suggest that WBSCR22 should be investigated further as potential therapeutic target in cancer cells.…”

Section: Discussionmentioning

confidence: 99%

The Human WBSCR22 Protein Is Involved in the Biogenesis of the 40S Ribosomal Subunits in Mammalian Cells

et al. 2013

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The human WBSCR22 protein was previously shown to be up-regulated in invasive breast cancer and its ectopic expression enhances tumor cell survival in the vasculature. In the current study, we show that the WBSCR22 protein is important for cell growth. Knock-down of WBSCR22 with siRNA results in slower growth of WBSCR22-depleted cells. Treatment with siWBSCR22 causes defects in the processing of pre-rRNAs and reduces the level of free 40S ribosomal subunit, suggesting that WBSCR22 is involved in ribosome small subunit biosynthesis. The human WBSCR22 partially complements the growth of WBSCR22 yeast homologue, bud23 deletion mutant suggesting that the human WBSCR22 is a functional homologue of yeast Bud23. WBSCR22 is localized throughout the cell nucleus and is not stably associated with ribosomal subunits within the cell nucleus. We also show that the WBSCR22 protein level is decreased in lymphoblastoid cell lines derived from William-Beuren Syndrome (WBS) patients compared to healthy controls. Our data suggest that the WBSCR22 protein is a ribosome biogenesis factor involved in the biosynthesis of 40S ribosomal particles in mammalian cells.

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Section: Discussionmentioning

confidence: 99%

The Human WBSCR22 Protein Is Involved in the Biogenesis of the 40S Ribosomal Subunits in Mammalian Cells

et al. 2013

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“…Nevertheless, the genelist already provides some interesting genes as candidate oncogenes whose oncogenic potential has been demonstrated in other tumor types. The genes include NOTCH1 [34], [35], [36], BMI1 [37], [38], [39], [40], [41], EFNA1 [42], [43], NCOA2 [44], BYSL [45], [46], and RAD21 [47]. For example, Notch1, a member of Notch family receptor has been indicated as an oncogene in multiple tumor types.…”

Section: Discussionmentioning

confidence: 99%

Integration of DNA Copy Number Alterations and Transcriptional Expression Analysis in Human Gastric Cancer

Fan

Dachrut

et al. 2012

PLoS ONE

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BackgroundGenomic instability with frequent DNA copy number alterations is one of the key hallmarks of carcinogenesis. The chromosomal regions with frequent DNA copy number gain and loss in human gastric cancer are still poorly defined. It remains unknown how the DNA copy number variations contributes to the changes of gene expression profiles, especially on the global level.Principal FindingsWe analyzed DNA copy number alterations in 64 human gastric cancer samples and 8 gastric cancer cell lines using bacterial artificial chromosome (BAC) arrays based comparative genomic hybridization (aCGH). Statistical analysis was applied to correlate previously published gene expression data obtained from cDNA microarrays with corresponding DNA copy number variation data to identify candidate oncogenes and tumor suppressor genes. We found that gastric cancer samples showed recurrent DNA copy number variations, including gains at 5p, 8q, 20p, 20q, and losses at 4q, 9p, 18q, 21q. The most frequent regions of amplification were 20q12 (7/72), 20q12–20q13.1 (12/72), 20q13.1–20q13.2 (11/72) and 20q13.2–20q13.3 (6/72). The most frequent deleted region was 9p21 (8/72). Correlating gene expression array data with aCGH identified 321 candidate oncogenes, which were overexpressed and showed frequent DNA copy number gains; and 12 candidate tumor suppressor genes which were down-regulated and showed frequent DNA copy number losses in human gastric cancers. Three networks of significantly expressed genes in gastric cancer samples were identified by ingenuity pathway analysis.ConclusionsThis study provides insight into DNA copy number variations and their contribution to altered gene expression profiles during human gastric cancer development. It provides novel candidate driver oncogenes or tumor suppressor genes for human gastric cancer, useful pathway maps for the future understanding of the molecular pathogenesis of this malignancy, and the construction of new therapeutic targets.

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“…For example, increased ribosome biogenesis caused by depletion of the cell-cycle control protein ADP ribosylation factor like 2 (Arl-2) also showed increases in nucleolar number, nucleolar area, and aggressivity of the tumor (Belin et al 2009). In addition, bystin-like (BYSL), a protein involved in pre-18S rRNA processing, may play a role in driving tumor formation as its inhibition has been shown to prevent tumor formation in nude mice (Wang et al 2009). Another way of examining how the nucleolus drives cancer is through ribosomopathies.…”

Section: Cancer and The Nucleolusmentioning

confidence: 99%

Determinants of mammalian nucleolar architecture

et al. 2015

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The nucleolus is responsible for the production of ribosomes, essential machines which synthesize all proteins needed by the cell. The structure of human nucleoli is highly dynamic and is directly related to its functions in ribosome biogenesis. Despite the importance of this organelle, the intricate relationship between nucleolar structure and function remains largely unexplored. How do cells control nucleolar formation and function? What are the minimal requirements for making a functional nucleolus? Here we review what is currently known regarding mammalian nucleolar formation at nucleolar organizer regions (NORs), which can be studied by observing the dissolution and reformation of the nucleolus during each cell division. Additionally, the nucleolus can be examined by analyzing how alterations in nucleolar function manifest in differences in nucleolar architecture. Furthermore, changes in nucleolar structure and function are correlated with cancer, highlighting the importance of studying the determinants of nucleolar formation.

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Bystin-like protein is upregulated in hepatocellular carcinoma and required for nucleologenesis in cancer cell proliferation

Cited by 34 publications

References 46 publications

The Human WBSCR22 Protein Is Involved in the Biogenesis of the 40S Ribosomal Subunits in Mammalian Cells

The Human WBSCR22 Protein Is Involved in the Biogenesis of the 40S Ribosomal Subunits in Mammalian Cells

Integration of DNA Copy Number Alterations and Transcriptional Expression Analysis in Human Gastric Cancer

Determinants of mammalian nucleolar architecture

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