Bystander effect of ultraviolet A radiation protects A375 melanoma cells by induction of antioxidant defense

Hansda, Surajit; Ghosh, Rita

doi:10.1080/26896583.2021.1994820

Cited by 3 publications

(4 citation statements)

References 41 publications

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“…The imbalances in peroxisomal H 2 O 2 metabolism have been associated with multiple oxidative stress-related human and animals disease states, and it can be linked to alterations in CAT activity [ 93 , 162 , 163 , 164 , 165 , 166 , 167 , 168 ]. CAT modulates the expression of numerous genes, i.e., CAT inhibition or overexpression can activate or inhibit the activity of NF-κB.…”

Section: Antioxidative Systems—the Main Player In Oxidative Response ...mentioning

confidence: 99%

“…It was shown to protect the heart from injury, dysfunction, and diseases, aging, and mortality, hypertension, albuminuria, tubulointerstitial fibrosis, and tubular apoptosis [ 93 ]. The overexpression of CAT may also dampen H 2 O 2 signalling and sensitise human and animal cells to different stressors (e.g., hepatocytes and fibroblasts, alveolar macrophages) or reduce the growth of cells (e.g., rat aortic smooth muscle cells, human aortic endothelial cells, human MCF-7 breast cancer cells, A-375 amelanotic melanoma cells, human promyelocytic HL-60 cells) [ 93 , 162 , 163 , 164 , 165 ]. In contrast, CAT inhibition increases oxidative damage, enhances metalloproteinases production, and impairs mitochondria functions [ 93 , 163 , 164 ].…”

Section: Antioxidative Systems—the Main Player In Oxidative Response ...mentioning

confidence: 99%

“…The overexpression of CAT may also dampen H 2 O 2 signalling and sensitise human and animal cells to different stressors (e.g., hepatocytes and fibroblasts, alveolar macrophages) or reduce the growth of cells (e.g., rat aortic smooth muscle cells, human aortic endothelial cells, human MCF-7 breast cancer cells, A-375 amelanotic melanoma cells, human promyelocytic HL-60 cells) [ 93 , 162 , 163 , 164 , 165 ]. In contrast, CAT inhibition increases oxidative damage, enhances metalloproteinases production, and impairs mitochondria functions [ 93 , 163 , 164 ]. Cancer cells frequently produce elevated levels of ROS which act as pro-tumorigenic signals that promote abnormal cell growth, migration, resistance to apoptosis (CD), adaptations to hypoxia, and genetic instability.…”

Section: Antioxidative Systems—the Main Player In Oxidative Response ...mentioning

confidence: 99%

See 2 more Smart Citations

To Be or Not to Be? Are Reactive Oxygen Species, Antioxidants, and Stress Signalling Universal Determinants of Life or Death?

Szechyńska‐Hebda

Ghalami

Kamran

et al. 2022

Cells

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In the environmental and organism context, oxidative stress is complex and unavoidable. Organisms simultaneously cope with a various combination of stress factors in natural conditions. For example, excess light stress is accompanied by UV stress, heat shock stress, and/or water stress. Reactive oxygen species (ROS) and antioxidant molecules, coordinated by electrical signalling (ES), are an integral part of the stress signalling network in cells and organisms. They together regulate gene expression to redirect energy to growth, acclimation, or defence, and thereby, determine cellular stress memory and stress crosstalk. In plants, both abiotic and biotic stress increase energy quenching, photorespiration, stomatal closure, and leaf temperature, while toning down photosynthesis and transpiration. Locally applied stress induces ES, ROS, retrograde signalling, cell death, and cellular light memory, then acclimation and defence responses in the local organs, whole plant, or even plant community (systemic acquired acclimation, systemic acquired resistance, network acquired acclimation). A simplified analogy can be found in animals where diseases vs. fitness and prolonged lifespan vs. faster aging, are dependent on mitochondrial ROS production and ES, and body temperature is regulated by sweating, temperature-dependent respiration, and gene regulation. In this review, we discuss the universal features of stress factors, ES, the cellular production of ROS molecules, ROS scavengers, hormones, and other regulators that coordinate life and death.

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Section: Antioxidative Systems—the Main Player In Oxidative Response ...mentioning

confidence: 99%

Section: Antioxidative Systems—the Main Player In Oxidative Response ...mentioning

confidence: 99%

Section: Antioxidative Systems—the Main Player In Oxidative Response ...mentioning

confidence: 99%

See 1 more Smart Citation

To Be or Not to Be? Are Reactive Oxygen Species, Antioxidants, and Stress Signalling Universal Determinants of Life or Death?

Szechyńska‐Hebda

Ghalami

Kamran

et al. 2022

Cells

View full text Add to dashboard Cite

show abstract

“…Additionally, while all cancers produce reactive oxygen species (ROS)—inherently subjecting their cells to oxidative stress conditions—and have well-developed antioxidant systems, the antioxidant capabilities of melanoma are superior. Melanocytes naturally contain various antioxidant substances that protect the skin from free radicals and oxidative stress [ 15 , 16 ]. Melanin precursors put melanocytes under continuous oxidative stress as they act as potent oxidants, but the melanosomes localise these substances, preventing damage to the cells.…”

Section: Melanomamentioning

confidence: 99%

The Potential of Antibody Technology and Silver Nanoparticles for Enhancing Photodynamic Therapy for Melanoma

2022

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Melanoma is highly aggressive and is known to be efficient at resisting drug-induced apoptotic signals. Resection is currently the gold standard for melanoma management, but it only offers local control of the early stage of the disease. Metastatic melanoma is prone to recurrence, and has a poor prognosis and treatment response. Thus, the need for advanced theranostic alternatives is evident. Photodynamic therapy has been increasingly studied for melanoma treatment; however, it relies on passive drug accumulation, leading to off-target effects. Nanoparticles enhance drug biodistribution, uptake and intra-tumoural concentration and can be functionalised with monoclonal antibodies that offer selective biorecognition. Antibody–drug conjugates reduce passive drug accumulation and off-target effects. Nonetheless, one limitation of monoclonal antibodies and antibody–drug conjugates is their lack of versatility, given cancer’s heterogeneity. Monoclonal antibodies suffer several additional limitations that make recombinant antibody fragments more desirable. SNAP-tag is a modified version of the human DNA-repair enzyme, O6-alkylguanine-DNA alkyltransferase. It reacts in an autocatalytic and covalent manner with benzylguanine-modified substrates, providing a simple protein labelling system. SNAP-tag can be genetically fused with antibody fragments, creating fusion proteins that can be easily labelled with benzylguanine-modified payloads for site-directed delivery. This review aims to highlight the benefits and limitations of the abovementioned approaches and to outline how their combination could enhance photodynamic therapy for melanoma.

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The Role of Bystander Effect in Ultraviolet A Induced Photoaging

Hansda

Ghosh

2022

Cell Biochem Biophys

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Background: Exposure to sunlight, mainly UVA, leads to typical changes in the features of the skin known as photoaging. UVA irradiation induces the expression of proteases that are responsible for the degradation of the extracellular matrix proteins to results in photoaging; it also downregulates the expression of proteins that are needed for the skin structure. Since, it is known that cells in the neighborhood of irradiated cells, but not directly exposed to it, often manifest responses like their irradiated counterparts, it is important to evaluate if these bystander cells too, can contribute to photoaging.Methods and Results: UVA induced cell cycle arrest have been associated with photoaging, from ow cytometry analysis we found that there was an induction of cell cycle arrest at the G 1 /S phase in the UVAbystander cells. The expression of some key photoaging marker genes likes, matrix metalloproteinases (MMP-1, MMP-3, MMP-9), cyclooxygenase-2 (COX-2), collagen1 and elastin were assessed from qRT-PCR.Upregulation of MMP-1 and COX-2, downregulation of collagen1 and elastin, along with suppression below normal expression for MMP-3 and MMP-9 was observed in the UVA-bystander A375 cells. Conclusion: Our ndings for the rst time suggest the contribution of UVA-bystander cells to the process of photoaging. HighlightsG 1 /S cell cycle arrest was observed in UVA-bystander A375 cells.The expression of MMP-1 & COX-2 was upregulated in UVA-bystander A375 cells.Collagen 1 & elastin expression was also downregulated in these bystander cells.Expression of MMP-3 & -9 in the UVA-bystander cells lower than that in control cells.

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Bystander effect of ultraviolet A radiation protects A375 melanoma cells by induction of antioxidant defense

Cited by 3 publications

References 41 publications

To Be or Not to Be? Are Reactive Oxygen Species, Antioxidants, and Stress Signalling Universal Determinants of Life or Death?

To Be or Not to Be? Are Reactive Oxygen Species, Antioxidants, and Stress Signalling Universal Determinants of Life or Death?

The Potential of Antibody Technology and Silver Nanoparticles for Enhancing Photodynamic Therapy for Melanoma

The Role of Bystander Effect in Ultraviolet A Induced Photoaging

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