BVES‐AS1 inhibits the malignant behaviors of colon adenocarcinoma cells via regulating BVES

Wen, Wu; Wang, Haiyan; Xie, Shuang; Wu, Zuyin; Zhang, Chunxu

doi:10.1002/cbin.11634

Cited by 4 publications

(3 citation statements)

References 27 publications

(31 reference statements)

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“… 24 It has also been reported that BVES-AS1 lncRNA inhibited colorectal adenocarcinoma progression via interacting with hsa-miR-522-3p. 25 In our study, hsa-miR-522-3p was induced 12.41-fold after treating with ST in H508 cells. On the contrary, there was no significant change in hsa-miR-522-3p expression after treating with ST in SNU-C4 cells.…”

Section: Discussionsupporting

confidence: 47%

CHRM3-Associated miRNAs May Play a Role in Bile Acid-Induced Proliferation of H508 Colon Cancer Cells

Aktan¹,

Teki̇n²,

Oruç³

et al. 2023

Turk J Gastroenterol

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Background: It was well defined that proliferative effects of bile acids on colon epithelium are through interaction with muscarinic-3 receptors. Recently, microRNA emerged as an important regulator of gene expression and has been implicated in pathogenesis of many malignancies. However, the interaction of CHRM3 and microRNAs and their potential effects on colon carcinogenesis remains to be elucidated. Methods: In the current study, analysis of cell proliferation for 6 days after treatment with sodium taurolithocholate was analyzed by using WST-1 method. microRNAs which possibly target CHRM3 were identified by in silico analyses. Expression profiling of these microRNAs, expression changes of CHRM3 gene at mRNA level for H508 and SNU-C4 colon cancer cells were analyzed by quantitative polymerase chain reaction; the protein level of CHRM3 was analyzed using Western blot; apoptotic experiments were analyzed using the Annexin V assay. The Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed using the miRPath v3.0. Results: It was found that the expression level of CHRM3 gene was 6.133 ± 0.698-fold in H508 cells compared with SNU-C4 cells ( P =.004). Treatment of H508 cells with sodium taurolithocholate caused 1.34 ± 0.4156-fold change in the expression level of CHRM3 gene ( P =.0448). No apoptotic changes were observed in both colon cancer cells after treatment with sodium taurolithocholate. Different expression changes were detected of hsa-miR-129-5p, hsa-miR-30c-5p, hsa-miR-224-5p, hsa-miR-30b-5p, hsa-miR-522-3p, and hsa-miR-1246. Finally, hsa-miR-1246 and hsa-miR-522-3p could play a critical role in tumor development via bile acid-related genes in colon cancer. Conclusion: These findings reflected that CHRM3 -dependent oncogenetic pathways might be in charge of colon cancer. We suggest that the microRNA expression profile of each individual colon cancer tissue is a unique digital signature.

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Section: Discussionsupporting

confidence: 47%

CHRM3-Associated miRNAs May Play a Role in Bile Acid-Induced Proliferation of H508 Colon Cancer Cells

Aktan¹,

Teki̇n²,

Oruç³

et al. 2023

Turk J Gastroenterol

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“…Previous work proved that lncRNA BVES-AS1 can operate as a tumor suppressor in CRC by repressing colon adenocarcinoma cell growth, motility, and metastasis through the miR-522-3p/BVES cascade [ 34 ]. Our current study suggests the tumor-promoting activity of BVES-AS1-201-50aa in CRC, indicating that BVES-AS1 and its peptide BVES-AS1-201-50aa have opposite effects in regulating colorectal tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

Peptide encoded by lncRNA BVES-AS1 promotes cell viability, migration, and invasion in colorectal cancer cells via the SRC/mTOR signaling pathway

et al. 2023

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Long non-coding RNAs (lncRNAs) have been revealed to harbor open reading frames (ORFs) that can be translated into small peptides. The peptides may participate in the pathogenesis of colorectal cancer (CRC). Herein, we investigated the role of a lncRNA BVES-AS1-encoded peptide in colorectal tumorigenesis. Through bioinformatic analysis, lncRNA BVES-AS1 was predicted to have encoding potential and to be associated with poor prognosis of patients with CRC. In CRC cells, BVES-AS1 was validated to encode a 50-aa-length micro-peptide, named BVES-AS1-201-50aa, through a western blotting method. BVES-AS1-201-50aa enhanced cell viability and promoted the migratory and invasive capacities of HCT116 and SW480 CRC cells in vitro, validated via CCK-8 assay and transwell assay, respectively. Immunofluorescence assay showed that BVES-AS1-201-50aa increased the expression of proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase 9 (MMP9) in CRC cells. We further verified that BVES-AS1-201-50aa targeted and activated the Src/mTOR signaling pathway in CRC cells by co-immunoprecipitation (Co-IP) experiment, qualitative proteomic analysis, and western blotting. Our findings demonstrated that BVES-AS1 could encode a micro-peptide, which promoted CRC cell viability, migration, and invasion in vitro. Our current work broadens the diversity and breadth of lncRNAs in human carcinogenesis.

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“…Regarding GI system, in colorectal cancer cell cultures, the interaction of long noncoding RNA blood vessel epicardial substance (BVES) antisense RNA 1 with miR-522-3p and TAF15 has been demonstrated to regulate BVES expression, which might offer a perspective for colorectal cancer treatment, but further study is needed[ 38 ]. In an elegant study, Tang et al [ 15 ] revealed significant upregulation of TAF15 in gastric cancer (GC) tumor tissues and cell lines.…”

Section: Taf15 In Gi Cancersmentioning

confidence: 99%

Tata-box-binding protein-associated factor 15 as a new potential marker in gastrointestinal tumors

Elpek

2024

World J Gastroenterol

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In this editorial, the roles of tata-box-binding protein-associated factor 15 (TAF15) in oncogenesis, tumor behavior, and as a therapeutic target in cancers in the context of gastrointestinal (GI) tumors are discussed concerning the publication by Guo et al . TAF15 is a member of the FET protein family with a comprehensive range of cellular processes. Besides, evidence has shown that TAF15 is involved in many diseases, including cancers. TAF15 contributes to carcinogenesis and tumor behavior in many tumors. Besides, its relationship with the mitogen-activated protein kinases (MAPK) signaling pathway makes TAF15 a new target for therapy. Although, the fact that there is few studies investigating the expression of TAF15 constitutes a potential limitation in GI system, the association of TAF15 expression with aggressive tumor behavior and, similar to other organ tumors, the influence of TAF15 on the MAPK signaling pathway emphasize that this protein could serve as a new molecular biomarker to predict tumor behavior and target therapeutic intervention in GI cancers. In conclusion, more studies should be performed to better understand the prognostic and therapeutic role of TAF15 in GI tumors, especially in tumors resistant to therapy.

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BVES‐AS1 inhibits the malignant behaviors of colon adenocarcinoma cells via regulating BVES

Cited by 4 publications

References 27 publications

CHRM3-Associated miRNAs May Play a Role in Bile Acid-Induced Proliferation of H508 Colon Cancer Cells

CHRM3-Associated miRNAs May Play a Role in Bile Acid-Induced Proliferation of H508 Colon Cancer Cells

Peptide encoded by lncRNA BVES-AS1 promotes cell viability, migration, and invasion in colorectal cancer cells via the SRC/mTOR signaling pathway

Tata-box-binding protein-associated factor 15 as a new potential marker in gastrointestinal tumors

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