Buyang Huanwu decoction up-regulates Notch1 gene expression in injured spinal cord

Abstract

Expression of genes in the Notch signaling pathway is altered in the injured spinal cord, which indicates that Notch participates in repair after spinal cord injury. Buyang Huanwu decoction, a traditional Chinese herbal preparation, can promote the growth of nerve cells and nerve fibers; however, it is unclear whether Buyang Huanwu decoction affects the Notch signaling pathway in injured spinal cord. In this study, a rat model was established by injuring the T10 spinal cord. At 2 days after injury, rats were intragastrically administered 2 mL of 0.8 g/mL Buyang Huanwu decoction daily until sacrifice. Real-time reverse transcription polymerase chain reaction analysis demonstrated that at 7, 14 and 28 days after injury, the expression of Notch1 was increased in the Buyang Huanwu decoction group compared with controls. These findings confirm that Buyang Huanwu decoction can promote the expression of Notch1 in rats with incomplete spinal cord injury, and may indicate a mechanism to promote the repair of spinal cord injury.

Sodium Tanshinone IIA Silate Exerts Microcirculation Protective Effects against Spinal Cord Injury In Vitro and In Vivo

“…One group [ 77 ] showed that the Notch ligand Jagged-1 (Jag1) present on endothelial cells is essential for neighboring vascular smooth muscle differentiation. Another group [ 78 ] demonstrated that the expression of Notch1 was increased after SCI and is upregulated when treated with drug. In this study, levels of Notch-1, Hes-1, and Jagged-1 proteins were significantly higher in the STS treated group compared to the SCI group 3 days after surgery.…”

Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-κB and MURF1 levels

“…In the present study, it was demonstrated that ANGPTL4 expression is involved in the therapeutic effect of BYHWT in a DSMA model. BYHWT could improve the blood circulation, inhibit fibroblast proliferation and decrease liquefaction and Wallerian degeneration, as well as enhance the nerve cells growth according to the previously published studies ( 11 , 14 , 15 ). ANGPTL4 is best known for its postprandial function in skeletal muscle lipid metabolism following a meal high in saturated fatty acids ( 8 ).…”

“…Buyang Huanwu Tang (BYHWT) has been used for treating hemiplegia, and is composed of Radix astragali , Semen Persicae, Carthami flos , Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Angelicae Sinensis and Pheretima ( 10 ). The BYHWT has been demonstrated to improve the blood circulation, inhibit fibroblast proliferation and decrease liquefaction and wallerian degeneration, as well as enhance nerve cell growth ( 11 ). Therefore, it is speculated that the BYHWT may participate in the pathogenesis of DSMA.…”