Buyang Huanwu Decoction can improve recovery of neurological function, reduce infarction volume, stimulate neural proliferation and modulate VEGF and Flk1 expressions in transient focal cerebral ischaemic rat brains

Cai, Guangyan; Liu, Baiyan; Liu, Wei; Tan, Xianhui; Rong, Jianhui; Chen, Xuemei; Li, Tong; Shen, Jiangang

doi:10.1016/j.jep.2007.06.007

Cited by 83 publications

(68 citation statements)

References 29 publications

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“…Many investigations showed the neuroprotective effects of BYHWD against cerebral ischaemiareperfusion injury (Cai et al, 2007;Wang et al, 2011;Zhao et al, 2012). The neuroprotection of BYHWD may involve different mechanisms, including increased nerve proliferation (Cai et al, 2007), improved nerve regeneration (Li et al, 2003), reduced inflammatory cytokine and neuronal apoptosis (Chen et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…The neuroprotection of BYHWD may involve different mechanisms, including increased nerve proliferation (Cai et al, 2007), improved nerve regeneration (Li et al, 2003), reduced inflammatory cytokine and neuronal apoptosis (Chen et al, 2001). These mechanisms are greatly associated with the pathophysiological process and clinical outcome in the acute and subacute phases.…”

Section: Discussionmentioning

confidence: 99%

“…BYHWD was prepared as follows (Cai et al, 2007). BYHWD is composed of Radix Astragali, Radix Angelicae Sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Semen Persicae, Flos Carthami, and Lumbricus, which were mixed at the ratio of 120:10:10:10:10:10:4.5 (dry weight).…”

Section: Composition and Preparation Of Byhwdmentioning

confidence: 99%

“…Focal cerebral ischemic rat models were induced by MCAO as previously reported (Cai et al, 2007;Xiong et al, 2013). In brief, the rats were anesthetized with 400 mg/kg of chloral hydrate i.p.…”

Section: Transient Focal Cerebra Ischaemia-reperfusion Modelmentioning

confidence: 99%

“…However, the mechanisms of this drug for stroke treatment are still unclear. Recent investigations showed its neuroprotective effects and mechanisms against cerebral ischaemia-reperfusion injury (Cai et al, 2007;Wang et al, 2011;Zhao et al, 2012). However, few studies have been performed to promote angiogenesis with BYHWD treatment, and the mechanisms of this drug for angiogenesis are unknown.…”

Section: Introductionmentioning

confidence: 99%

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Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia

Shen

Zhu

Hai

et al. 2014

J. Zhejiang Univ. Sci. B

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Buyang Huanwu decoction (BYHWD), a traditional Chinese herbal prescription, has been widely used clinically to treat stroke in China for hundreds of years; however, the mechanisms of this drug for stroke treatment are still unclear. This study aims to observe the cerebral angiogenesis effects of BYHWD on chronic brain injury after focal cerebral ischemia in rats and to explore its possible mechanisms. The ischemia was induced by occlusion of the right middle cerebral artery for 90 min. BYHWD (12.5 and 25.0 g/(kg·d), equivalent to the dry weight of the raw materials) was orally administered twice a day beginning 2 h after surgery. BYHWD significantly attenuated the neurological dysfunction, infarct volume, and brain atrophy after ischemia. There was a significant increase in the microvessel density, as assessed by immunofluorescence CD31, and a significant increase in angiopoietin-1 (Ang-1) in the penumbra areas of the rats was shown by immunohistochemical staining and Western blotting. The results indicate that the neurorestorative effects of BYHWD are associated with angiogenesis and the enhancement of the expressions of Ang-1 on chronic brain injury after focal cerebral ischemia.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Composition and Preparation Of Byhwdmentioning

confidence: 99%

Section: Transient Focal Cerebra Ischaemia-reperfusion Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia

Shen

Zhu

Hai

et al. 2014

J. Zhejiang Univ. Sci. B

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Simultaneous determination of twelve bioactive constituents in Buyang Huanwu decoction by HPLC‐DAD‐ELSD and HPLC‐TOF/MS

Liu

Cheng

et al. 2009

Biomedical Chromatography

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Buyang Huanwu Decoction (BYHYD) is a classical traditional Chinese medicinal prescription that has been widely used for treating cerebrovascular illnesses for hundreds of years. In this study, a comprehensive analytical method has been developed for quantitative analysis of the major constituents in BYHWD. This method was based on high-performance liquid chromatography coupled to a diode array and evaporative light scattering detectors (HPLC-DAD-ELSD) on a common reverse-phase C(18) column. HPLC coupled with on-line time-of-flight mass spectrometry (HPLC-TOF/MS) was additionally adopted to provide further validation for the constituents. It was found that 0.3% aqueous formic acid and acetonitrile was the optimum mobile phase for gradient elution. This method, which showed excellent precision and accuracy, was successfully applied to quantify the bioactive constituents in six BYHWD products. The validated HPLC-DAD-ELSD method, together with the HPLC-TOF/MS analysis, provided a new basis for assessing the quality of traditional Chinese medicinal prescription consisting of many bioactive components.

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Determination of paeoniflorin, calycosin‐7‐O‐β‐d‐glucoside, ononin, calycosin and formononetin in rat plasma after oral administration of Buyang Huanwu decoction for their pharmacokinetic study by liquid chromatography–mass spectrometry

Zhang

Yang

Lin

et al. 2010

Biomedical Chromatography

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The purpose of this study was to simultaneously investigate the pharmacokinetics of five bioactive compounds in rat plasma after oral administration of Buyang Huanwu decoction (BYHWD) using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The separations were performed on a Thermo Hypersil Gold C(18) analytical column (50 × 2.1 mm, 3 µm) with the column temperature kept at 30°C. The quantitative analysis was performed using a quadrupole mass spectrometer detector operated under selected ion monitoring mode. A linear gradient elution of A (0.1% formic acid solution) and B (100% acetonitrile) was used at a flow rate of 0.2 mL/min. The method was validated within the concentration ranges 1.8-450, 6.0-1500, 2.0-500, 1.2-300 and 1.2-150 ng/mL for paeoniflorin, calycosin-7-O-β-d-glucoside, ononin, calycosin and formononetin, respectively. The calibration curves were linear with correlation coefficients > 0.99. The lower limits of quantitations were < 6.0 ng/mL. The method was further applied to assess the pharmacokinetics of the five bioactive constituents of BYHWD in rat plasma.

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Buyang Huanwu Decoction can improve recovery of neurological function, reduce infarction volume, stimulate neural proliferation and modulate VEGF and Flk1 expressions in transient focal cerebral ischaemic rat brains

Cited by 83 publications

References 29 publications

Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia

Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia

Simultaneous determination of twelve bioactive constituents in Buyang Huanwu decoction by HPLC‐DAD‐ELSD and HPLC‐TOF/MS

Determination of paeoniflorin, calycosin‐7‐O‐β‐d‐glucoside, ononin, calycosin and formononetin in rat plasma after oral administration of Buyang Huanwu decoction for their pharmacokinetic study by liquid chromatography–mass spectrometry

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