Butyric Acid Fermentation in Xylose and Glucose by Clostridium tyrobutyricum

Luo, Guanghong; Zhang, Ling; Chen, Tianren; Yuan, Wenqiao; Geng, Yingxi

doi:10.15376/biores.12.2.2930-2940

Cited by 8 publications

(14 citation statements)

References 26 publications

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“…C. tyrobutyricum also has the capacity to use xylose as a sole carbon and energy source. The genome encodes xylose isomerase and xylulose kinase genes, which are tightly regulated by carbon catabolite repression mechanisms (discussed further below) (Fu et al, 2017b;Luo et al, 2017).…”

Section: Tyrobutyricum Genome Architecture and Central Carbon Metamentioning

confidence: 99%

“…While xylose is a known substrate for C. tyrobutyricum, xylose consumption is impeded by CCR, as indicated above (Fu et al, 2017b;Luo et al, 2017). In order to bypass host CCR, Fu et al (Fu et al, 2017b) heterologously overexpressed xylose catabolism genes xylT, xylA, and xylB from C. acetobutylicum.…”

Section: Tyrobutyricum Metabolic Engineeringmentioning

confidence: 99%

“…Other research has examined the impact that media optimization, additives, or novel renewable feedstock on C tyrobutyricum. Diverse strategies include optimizing growth with varying concentrations of glucose or xylose ( Table 1; Luo et al, 2017), the enhancing capabilities of small arabinose concentrations on metabolism in sugars media and corncob hydrolysate (Chen et al, 2017), and the optimal ratio of rice straw and brown algae to enhance productivity as both components supplement different sugar needs for optimal growth (Oh et al, 2019b). The addition of additives have also shown some promise in enhancing fermentation yields.…”

Section: Fermentation Optimization and Media Researchmentioning

confidence: 99%

See 2 more Smart Citations

Development of Clostridium tyrobutyricum as a Microbial Cell Factory for the Production of Fuel and Chemical Intermediates From Lignocellulosic Feedstocks

et al. 2020

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Microbial conversion of lignocellulosic substrates to fuel and platform chemical intermediates offers a sustainable route to establish a viable bioeconomy. However, such approaches face a series of key technical, economic, and sustainability hurdles, including: incomplete substrate utilization, lignocellulosic hydrolysate, and/or endproduct toxicity, inefficient product recovery, incompatible cultivation requirements, and insufficient productivity metrics. Development of a production host with native traits suitable for high productivity conversion of lignocellulosic substrates under process-relevant conditions offers a means to bypass the above-described hurdles and accelerate the development of microbial biocatalyst deployment. Clostridium tyrobutyricum, a native producer of short chain fatty acids, displays a series of characteristics that make it an ideal candidate for conversion of lignocellulosic substrates and thus represents a promising host for microbial production of diverse carboxylate-derived product suites. Herein, recent progress and future directions in the development of this bacterium as an industrial microbial cell factory, with emphases on the utilization of lignocellulosic substrates and metabolic engineering approaches, is reviewed.

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Section: Tyrobutyricum Genome Architecture and Central Carbon Metamentioning

confidence: 99%

Section: Tyrobutyricum Metabolic Engineeringmentioning

confidence: 99%

Section: Fermentation Optimization and Media Researchmentioning

confidence: 99%

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Development of Clostridium tyrobutyricum as a Microbial Cell Factory for the Production of Fuel and Chemical Intermediates From Lignocellulosic Feedstocks

et al. 2020

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“…However, we do know that starch gelatinization breaks down the intermolecular bonds of starch molecules and increases the glucose content. Intestinal microflora can utilize glucose as a carbon source for acetic acid, butyric acid and other volatile fatty acid production (Fu et al 2017;Luo et al 2017). Butyric acid is the main source of epithelial cell growth and can repair damage to promote the development of normal epithelial cells (Knudsen et al 2012).…”

Section: Springiness (×1 000 G)mentioning

confidence: 99%

Effects of conditioners (single-layer, double-layer and retention-conditioner) on the growth performance, meat quality and intestinal morphology of growing and finishing pigs

Duan

Xue

et al. 2018

Journal of Integrative Agriculture

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This experiment was conducted to investigate the effects of feed conditioners (single-layer, double-layer and retentionconditioner) on the growth performance, meat quality and intestinal morphology of pigs throughout the growing to finishing phase. A total of 96 growing pigs ((28.70±3.20) kg) were selected and randomized into three treatment groups with four replicates per group. Eight pigs were used per replicate for the 17-week feeding trial. The grower diet was given at 0 to 6 weeks and a finisher diet was given at 6 to 17 weeks. The treatments were as follows: SC diet (control; single-layer conditioning), DC diet (double-layer conditioning), and RC diet (retention-conditioning). Starch gelatinization was significantly higher (P<0.05) in the RC treatment than in the SC treatment, however, there was no significant difference in the starch gelatinization between the DC group and the RC group. In the growing phase, the feed to gain index (F:G) was significantly lower (P<0.05) in the RC group than in the SC and DC groups. Between growing and finishing, the F:G was the lowest (P<0.05) in the SC group compared to the RC or DC group. Drip loss, a measurement of meat quality, was significantly lower (P<0.05) in longissimus dorsi tissue collected from pigs fed the RC diet than in tissues collected from pigs fed the SC diet. The intestinal quality of the duodenum and jejunum tissues showed a significant increase (P<0.05) in the crypt depth and villus height in the RC group compared to the SC-or DC-treated pigs. These results demonstrated that the retentionconditioner treatment decreased the F:G in growing pigs, improved intestinal morphology and enhanced the meat quality in the finishing pigs. However, the retention-conditioner treatment had a negative impact on growth performance in the finishing pigs.

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“…Some work has been reported to convert cellulose and part of hemicellulose (mainly xylose) to butyric acid by C. tyrobutyricum (Zhu et al 2002;2004;Jiang et al 2009;Mitchell et al 2009;Luo et al 2017). Butyric acid is a short-chain fatty acid (C3H7COOH), which has many applications, including use in the production of butanol, ethyl butyrate, and butyl butyrate (Al-Shorgani et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Butyric acid production by Clostridium tyrobutyricum in sugar mixtures and corncob hydrolysate containing arabinose

Chen

Zhang²,

Luo

et al. 2017

BioRes

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The objective of this study was to understand arabinose effects on butyric acid fermentation by Clostridium tyrobutyricum in sugar-supplemented media and biomass hydrolysate. Compared to glucose and xylose, the conversion of arabinose to butyric acid was less efficient with a longer lag phase (4 d) and fermentation period (6 d), and a lower butyric acid yield of 0.26 g/g and 0.15 g/g in 5 g/L and 10 g/L of arabinose media, respectively. However, the addition of 2 g/L or 5 g/L of arabinose to the baseline medium that contained glucose (15 g/L) and xylose (15 g/L) enhanced butyric acid synthesis, resulting in 16.1 g/L and 20.3 g/L of butyric acid, respectively. Further increase in arabinose to 10 g/L in the medium of 10 g/L of glucose and 10 g/L of xylose showed inhibitory effects on C. tyrobutyricum, which suggested that high concentrations of arabinose (> 10 g/L) were not desirable. Dilute-acid pretreated corncobs that contained xylose (19 g/L) and a small amount of arabinose (2 g/L) were a feasible substrate for butyric acid production by C. tyrobutyricum, and resulted in 10.6 g/L butyric acid, which was slightly lower than in the mimic medium (11.3 g/L).

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Butyric Acid Fermentation in Xylose and Glucose by Clostridium tyrobutyricum

Cited by 8 publications

References 26 publications

Development of Clostridium tyrobutyricum as a Microbial Cell Factory for the Production of Fuel and Chemical Intermediates From Lignocellulosic Feedstocks

Development of Clostridium tyrobutyricum as a Microbial Cell Factory for the Production of Fuel and Chemical Intermediates From Lignocellulosic Feedstocks

Effects of conditioners (single-layer, double-layer and retention-conditioner) on the growth performance, meat quality and intestinal morphology of growing and finishing pigs

Butyric acid production by Clostridium tyrobutyricum in sugar mixtures and corncob hydrolysate containing arabinose

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