Butyrate promotes C2C12 myoblast proliferation by activating ERK/MAPK pathway

Guan, Li; Cao, Ziping; Pan, Ziyue; Zhao, Chao; Xue, Mengzhou; Yang, Fan; Chen, J. Y.

doi:10.1039/d2mo00256f

Cited by 7 publications

(5 citation statements)

References 40 publications

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“…41 Additionally, they modulated IL-10-producing Treg cell differentiation in gutassociated lymphoid tissues, 42 facilitating mucosal immunity, while reducing pro-inflammatory cytokines and restoring mitochondrial function in muscle cells. Furthermore, butyrate could recognize and activate the G protein-coupled receptors GRP41 (FFAR3) and GRP43(FFAR2), 43 promoting glucose metabolism, mitochondrial biogenesis, and myoblast proliferation, and modulate age-related muscle atrophy by activating the ERK/ MAPK pathway, 44 or inhibiting histone deacetylase (HDAC) 45 or other pathways. This contributes to increasing the CSA of muscle fiber, preventing intramuscular fat accumulation, and finally improving physical frailty and sarcopenia.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lactobacillus plantarum BFS1243 on a female frailty model induced by fecal microbiota transplantation in germ-free mice

Dong,

Zeng,

et al. 2024

Food Funct.

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Section: Discussionmentioning

confidence: 99%

Effect of Lactobacillus plantarum BFS1243 on a female frailty model induced by fecal microbiota transplantation in germ-free mice

Dong,

Zeng,

et al. 2024

Food Funct.

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“…Interestingly, Clostridium sensu stricto 1 , identified as a producer of short-chain fatty acids (SCFAs), aligns with research demonstrating the muscle-protective benefits of dietary SCFA intake in older adults [ 59 , 60 ]. Butyrate, one of the representative SCFAs, promotes C2C12 myoblast proliferation [ 61 ] and alleviates muscle atrophy by enhancing gut barrier function and activating the PI3K/Akt/mTOR signaling cascade [ 62 ]. Therefore, further investigations are warranted to elucidate the potential protective role of Clostridium sensu stricto 1 in the context of muscle atrophy.…”

Section: Discussionmentioning

confidence: 99%

Cornflower Extract and Its Active Components Alleviate Dexamethasone-Induced Muscle Wasting by Targeting Cannabinoid Receptors and Modulating Gut Microbiota

Nguyen,

Le,

Kang

et al. 2024

Nutrients

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Sarcopenia, a decline in muscle mass and strength, can be triggered by aging or medications like glucocorticoids. This study investigated cornflower (Centaurea cyanus) water extract (CC) as a potential protective agent against DEX-induced muscle wasting in vitro and in vivo. CC and its isolated compounds mitigated oxidative stress, promoted myofiber growth, and boosted ATP production in C2C12 myotubes. Mechanistically, CC reduced protein degradation markers, increased mitochondrial content, and activated protein synthesis signaling. Docking analysis suggested cannabinoid receptors (CB) 1 and 2 as potential targets of CC compounds. Specifically, graveobioside A from CC inhibited CB1 and upregulated CB2, subsequently stimulating protein synthesis and suppressing degradation. In vivo, CC treatment attenuated DEX-induced muscle wasting, as evidenced by enhanced grip strength, exercise performance, and modulation of muscle gene expression related to differentiation, protein turnover, and exercise performance. Moreover, CC enriched gut microbial diversity, and the abundance of Clostridium sensu stricto 1 positively correlated with muscle mass. These findings suggest a multifaceted mode of action for CC: (1) direct modulation of the muscle cannabinoid receptor system favoring anabolic processes and (2) indirect modulation of muscle health through the gut microbiome. Overall, CC presents a promising therapeutic strategy for preventing and treating muscle atrophy.

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“…In addition, some studies have shown that butyric acid can activate AMPK signaling pathways, stimulate mitochondrial fatty acid oxidation, enhance the glucose metabolism in muscle cells, reduce lipid deposition in muscles, and thereby improve muscle quality and function [18]. It may also promote the proliferation of C2C12 myoblasts by upregulating ERK phosphorylation, in which the expression of myogenic regulatory factors including Myf5 and MyoD are significantly increased, thereby regulating the activation of muscle satellite cells [35]. In one…”

Section: Discussionmentioning

confidence: 99%

Supplementation with Lactiplantibacillus brevis GKEX Combined with Resistance Exercise Training Improves Muscle Mass, Strength Performance, and Body Fat Condition in Healthy Humans

Lee,

Hsu,

et al. 2024

Foods

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In addition to maintaining good exercise and dietary habits, recent studies have shown that probiotics may have potential benefits for muscle mass and strength. It is worth noting that the effects may vary depending on the specific strains used. To date, no studies have analyzed the effects of Lactiplantibacillus brevis in this context. Here, we combine the L. brevis strain GKEX with resistance training to further understand its effects on muscle mass, thickness, performance, and fat loss. In a six-week intervention for a double-blind randomized trial, 52 healthy subjects were divided into two groups (10 male and 16 female participants in each group): a placebo group (two capsules/day, containing 0 CFU of GKEX per capsule) and a GKEX group (two capsules/day, containing 1 × 1010 CFU of GKEX per capsule). Before the intervention, no differences were observed between the two groups in any of the tests (body composition, muscle thickness, exercise performance, and blood parameters). However, supplementation with GKEX significantly improved muscle mass and thickness, as well as grip strength, muscle strength, and explosive performance, when compared to the associated parameters before the intervention. Additionally, GKEX supplementation promoted a reduction in the body fat percentage (p < 0.05). Through analysis of the change amount, we observed that GKEX supplementation yielded significantly improved benefits when compared to the placebo group (p < 0.05). In summary, our findings support the notion that a six-week resistance exercise training program combined with L. brevis GKEX supplementation has superior additive effects that enhance muscle mass and strength performance, while also reducing body fat percentage. This intervention can promote muscle gain and fat loss.

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Butyrate promotes C2C12 myoblast proliferation by activating ERK/MAPK pathway

Abstract: The role of butyrate, a metabolite of intestinal bacteria, in sarcopenia has received much attention in recent years.

Cited by 7 publications

References 40 publications

Effect of Lactobacillus plantarum BFS1243 on a female frailty model induced by fecal microbiota transplantation in germ-free mice

Effect of Lactobacillus plantarum BFS1243 on a female frailty model induced by fecal microbiota transplantation in germ-free mice

Cornflower Extract and Its Active Components Alleviate Dexamethasone-Induced Muscle Wasting by Targeting Cannabinoid Receptors and Modulating Gut Microbiota

Supplementation with Lactiplantibacillus brevis GKEX Combined with Resistance Exercise Training Improves Muscle Mass, Strength Performance, and Body Fat Condition in Healthy Humans

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