Butyrate, mesalamine, and factor XIII in experimental colitis in the rat: Effects on transglutaminase activity

D’Argenio, Giuseppe; Cosenza, V.; Sorrentini, I.; Ritis, Francesca De; Gatto, Alfonso; Cave, M. Delle; D’Armiento, Francesco Paolo; Mazzacca, G

doi:10.1016/0016-5085(94)90598-3

Cited by 64 publications

(25 citation statements)

References 32 publications

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“…Previous studies of the involvement of F-XIII in the wound healing process have measured only plasma activity [17,18]. From our results, the plasma F-XIII activity of Alb animals was within the normal range, whereas the anastomotic tensile strength was remarkably reduced.…”

Section: Discussionsupporting

confidence: 63%

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

Hasegawa¹,

Eguchi

Endoh³

et al. 2004

Eur Surg Res

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Background: Blood coagulation factor XIII (F-XIII) promotes cross-linking of fibrin during blood coagulation. Impaired clot stabilization in patients with genetic deficiencies of F-XIII is associated with marked pathologies of wound healing. Methods: 60 rats given carbon tetrachloride underwent ileal anastomosis after which they received albumin (Alb animals) or F-XIII concentrate (F-XIII animals) immediately after surgery and daily thereafter until day 2 or 4 and were euthanized on day 3 or 5, respectively, or until day 5 and were euthanized on day 7, 10, or 21. We measured the plasma F-XIII activity and anastomotic tensile strength, followed by immunohistochemical localization of F-XIII subunit A within anastomoses. Results: On day 3, there were no significant differences between Alb and F-XIII animals for plasma F-XIII activity and tensile strength, and both groups of animals showed little immunostaining for F-XIII on anastomoses. Plasma F-XIII activities did not differ between Alb and F-XIII animals on day 5 (115.8 ± 16.8 vs. 137.3 ± 14.9%). Although the tensile strength in both groups was increased compared with that of day 3, that in F-XIII animals (129.8 ± 3.3 gf) was significantly higher than that in Alb animals (100.8 ± 5.3 gf, p = 0.014). F-XIII animals showed de novo collagen fibers and intense immunoreactivity of F-XIII in the extracellular matrix around the anastomoses. Similar differences occurred on day 7 but not days 10 and 21. Conclusions: F-XIII concentrate may accelerate the early healing process of intestinal anastomosis because of the protein’s accumulation in situ.

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Section: Discussionsupporting

confidence: 63%

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

Hasegawa¹,

Eguchi

Endoh³

et al. 2004

Eur Surg Res

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“…Thus, different studies have shown that topical SCFA treatment can effectively be used to treat distal ulcerative colitis, diversion colitis, nonspecific protosigmoiditis in humans (Breuer et al 1991;Harig et al 1989;Scheppach et al 1992;Senagore et al 1992), and experimental bowel inflammation in rats (Butzner et al 1996;D'Argenio et al 1994). In consequence, butyrate (or other SCFA) could actively participate in epithelium regeneration induced after colonic damage.…”

Section: Introductionmentioning

confidence: 99%

The effects of short-chain fatty acids on colon epithelial proliferation and survival depend on the cellular phenotype

Comalada

Bailón

Haro

et al. 2006

J Cancer Res Clin Oncol

176

125

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“…Activation of IL-8 expression is significantly diminished in Caco-2 cells after spontaneous differentiation by growth to a post-confluent state or after treatment with compounds that inhibit cell proliferation and induce markers of a differentiated phenotype such as vitamin D and sodium butyrate [12]. The effect of butyrate on IL-8 gene expression was of particular interest to us because indirect evidence suggests that it may normally play an important role in modulating the immune response of the colonic mucosa [14][15][16][17][18][19][20]. The only tissue in vertebrates that is normally exposed to substantial levels of butyrate is the mucosa of the colon through the fermentation of carbohydrates by the normal colonic bacterial flora [21,22].…”

Section: Introductionmentioning

confidence: 99%

High-level expression of IkB-b in the surface epithelium of the colon: in vitro evidence for an immunomodulatory role

Huang

Wen

et al. 1999

Journal of Leukocyte Biology

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The intestinal epithelium is spatially segregated into two compartments, one containing undifferentiated cells in a proliferative state and one with non-proliferative differentiated cells. Although this epithelium can produce many immunemodulating substances, emerging evidence suggests that the differentiated cell compartment is less immune responsive. Indeed, it is the differentiated cellular compartment that represents the interface between the highly antigenic luminal environment and the mucosal immune system. The NF-B/rel family of transcriptional activators play a critical role in regulating the inflammatory response by activating a wide variety of immune-modulating genes. These transcription factors are maintained in an inactive state in the cytoplasmic compartment by interaction with inhibitory proteins of the I B family. In this study we show by immunohistochemistry that I B-␤ is expressed at high levels specifically in the differentiated surface epithelium of the colonic mucosa. Using a naturally occurring compound found in the colon of vertebrates, butyrate, we provide evidence in an intestinal cell line that alteration of I B-␤ expression can modulate the transcriptional activation of the interleukin-8 (IL-8) gene by preventing the nuclear translocation of NF-B proteins. Therefore, the expression of I B-␤ in the differentiated surface epithelium of the colon may help these cells act as an immunological barrier to prevent activation of the mucosal immune system.

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Butyrate, mesalamine, and factor XIII in experimental colitis in the rat: Effects on transglutaminase activity

Cited by 64 publications

References 32 publications

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

The effects of short-chain fatty acids on colon epithelial proliferation and survival depend on the cellular phenotype

High-level expression of IkB-b in the surface epithelium of the colon: in vitro evidence for an immunomodulatory role

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