Butylated hydroxytoluene can reduce oxidative stress and improve quality of frozen–thawed bull semen processed in lecithin and egg yolk based extenders

Khumran, A.M.; Yimer, Nurhusien; Rosnina, Y.; Ariff, M.O.; Wahid, H.; Kaka, Asmatullah; Ebrahimi, Mahdi; Sarsaifi, Kazhal

doi:10.1016/j.anireprosci.2015.10.007

Cited by 28 publications

(25 citation statements)

References 16 publications

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“…Sperm concentration and the total motility of the ejaculate were assessed using a spectrophotometer (Spermacue ® ) and an optical microscope at 400× magnification (Eclipse E200 ® , Nikon Inc), respectively. The minimum requirements to include an ejaculate in the present study were 70% of total motility and 500 × 10 6 ml −1 of sperm concentration (Khumran et al, ).…”

Section: Methodsmentioning

confidence: 96%

Membrane stability and mitochondrial activity of bovine sperm frozen with low‐density lipoproteins and trehalose

Varela

Rojas

Betancur

2019

Reprod Domestic Animals

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Cryopreservation results in the destabilization of the sperm plasma membrane, leading to negative side effects such as premature cryocapacitation, apoptosis and the low mitochondrial activity of bovine spermatozoa. Low‐density lipoproteins (LDL) and trehalose have been used in seminal freezing to protect the integrity and stability of sperm membranes. Likewise, trehalose can increase the mitochondrial activity of sperm. The objective of this study was to evaluate the membrane stability and mitochondrial activity of bovine sperm after being frozen and treated with LDL sources and trehalose. Ten ejaculates from five bulls were cryopreserved under the treatments, CEY: chicken egg yolk (20% v/v); CCEY: centrifuged CEY (20% v/v); LDL: LDL (8% v/v); T: trehalose (100 mM); and TLDL: T (100 mM) plus LDL (8% v/v). After thawing, membrane stability and mitochondrial membrane potential (ΔΨM) were assessed by flow cytometry through the M‐540/Yopro‐1 and DiOC6/PI probes. The structural membrane integrity (SMI) was evaluated by fluorescence microscopy using SYBR14/PI dyes. A generalized linear model was adjusted, and the means were compared using the Tukey test. Centrifuged chicken egg yolk and LDL had a higher proportion of non‐cryocapacitated non‐apoptotic sperm (M−Y−), while CEY and T had the largest populations of cryocapacitated non‐apoptotic sperm (M+Y−) and cryocapacitated apoptotic sperm (M+Y+). Centrifuged chicken egg yolk also showed a higher proportion of sperm with high‐ΔΨM. Treatments that included egg yolk or purified LDL had a positive effect on SMI. Centrifuged chicken egg yolk has a superior cryoprotective effect on membrane stability and mitochondrial activity of bovine semen over the conventional use of CEY or the individual or simultaneous use of LDL and trehalose.

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Section: Methodsmentioning

confidence: 96%

Membrane stability and mitochondrial activity of bovine sperm frozen with low‐density lipoproteins and trehalose

Varela

Rojas

Betancur

2019

Reprod Domestic Animals

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“…A total of 24 ejaculates were collected, and the samples were stored in a cooler box containing warm water at 37°C and transported to the laboratory for evaluations. Ejaculate samples collected with ≥80% normal sperm morphology, ≥70% general motility, and semen concentration of ≥500 × 10 6 /ml [20] were included in the experiment. The semen samples were then extended with fraction one of the Tris (2.42 g) - citric (1.48 g) - fructose (1 g) extenders [21] as the main extender with different percentage of SL (SL P5638, Sigma–Aldrich, USA) (0%, 1%, 1.25%, 1.5%, and 1.75%) containing 2% VCO (VCO; manufactured by Nano Xan Sdn.…”

Section: Methodsmentioning

confidence: 99%

“…In the freezing step, the straws were put on racks and placed horizontally 4 cm above the surface of the liquid nitrogen for 10 min. The racks were left to plunge with the straws on liquid nitrogen for 3 min before immersing them into the liquid nitrogen [20]. …”

Section: Methodsmentioning

confidence: 99%

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Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

et al. 2017

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Aim:The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters.Materials and Methods:A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender which consists of various concentrations of SL (1, 1.25, 1.5, and 1.75%). A 20% egg yolk in Tris used as a positive control (C+). The diluted semen samples were divided into two fractions; one for chilling which were stored at 4°C for 24, 72, and 144 h before evaluated for semen quality parameters. The second fraction used for freezing was chilled for 3 h at 4°C, packed into 0.25 mL straws and then cryopreserved in liquid nitrogen. The samples were then evaluated after 7 and 14 days. Chilled and frozen semen samples were thawed at 37°C and assessed for general motility using computer-assisted semen analysis, viability, acrosome integrity and morphology (eosin-nigrosin stain), membrane integrity, and lipid peroxidation using thiobarbituric acid reaction test.Results:The results showed that all the quality parameters assessed were significantly (p<0.05) improved at 1.5% SL concentration in chilled semen. Treatment groups of 1, 1.25, 1.5, and 1.75% SL were higher in quality parameters than the control group (C+) in chilled semen. However, all the quality parameters in frozen-thawed semen were significantly higher in the C+ than the treated groups.Conclusion:In conclusion, supplementation of 1.5% SL in 2% VCO Tris-based extender enhanced the chilled bull semen. However, there was no marked improvement in the frozen-thawed quality parameters after treatment.

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“…Esse resultado difere do encontrado por Ghorbani et al (19) em humanos, pois relataram que concentrações de BHT superiores a 0,5 mM resultaram em menor motilidade e viabilidade espermática provavelmente devido a um aumento de ânions superóxido e H2O2 nos espermatozoides. Khumran et al (26) relataram uma toxicidade causada pelo BHT, quando este é utilizado em concentrações maiores de 1 mM em sêmen de touros, levando a redução da motilidade e da viabilidade espermática. O mecanismo relacionado à toxicidade deste antioxidante ainda não se encontra totalmente elucidado, mas, ao que parece, o espermatozoide do cão parece ser mais resistente a este efeito deletério.…”

Section: Discussionunclassified

ADIÇÃO DE HIDROXITOLUENO BUTILADO (BHT) NO DILUIDOR ACP-106c PARA CONGELAÇÃO DE SÊMEN CANINO

et al. 2018

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Resumo:Este estudo foi realizado para determinar o efeito de hidroxitolueno butilado (BHT) sobre a qualidade do sêmen canino congelado e descongelado, utilizando o diluidor à base de água de coco em pó (ACP-106c). Para tanto, foram realizadas quinze coletas de sêmen provenientes de cinco cães. O sêmen obtido foi diluído em ACP-106c acrescido de glicerol e gema de ovo. As amostras foram então transferidas para tubos contendo diferentes concentrações de BHT (0; 0,5; 1,0 e 2,0 mM). Em seguida, as amostras foram envasadas, congeladas e armazenadas em nitrogênio líquido. O sêmen coletado foi avaliado in natura quanto aos seguintes parâmetros: coloração, volume da fração espermática, motilidade total, vigor, concentração, morfologia e funcionalidade de membrana espermática. Após uma semana, as amostras foram descongeladas e avaliadas por meio de análise computadorizada, como também foram realizadas análises da funcionalidade de membrana e da morfologia espermática. A motilidade progressiva no grupo BHT 2,0 mM foi significativamente superior (P < 0,05) do que a do grupo BHT 0 mM (27,6 ± 11,7% vs. 19,0 ± 9,5%, respectivamente). Em todos os demais parâmetros avaliados, não houve diferença entre os grupos testados. Portanto, conclui-se que a adição do BHT ao diluidor ACP-106c não afetou a qualidade do sêmen canino pós-descongelação. Abstract:This study was conducted to determine the effect of butylated hydroxytoluene (BHT) on the quality of canine sperm frozen and thawed using the powdered coconut water based (ACP-106c) extender. Therefore, fifteen ejaculates were collected from five dogs. Semen obtained was diluted in ACP-106c added of glycerol and egg yolk. The samples were then transferred to tubes containing different concentrations of BHT (0, 0.5, 1.0 and 2.0 mM). After that, the samples were filled into straws, frozen and stored in liquid nitrogen. Fresh semen was evaluated for the following parameters: color, sperm fraction volume, total motility, vigor, concentration, morphology, and HOST test. After one week,

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Butylated hydroxytoluene can reduce oxidative stress and improve quality of frozen–thawed bull semen processed in lecithin and egg yolk based extenders

Cited by 28 publications

References 16 publications

Membrane stability and mitochondrial activity of bovine sperm frozen with low‐density lipoproteins and trehalose

Membrane stability and mitochondrial activity of bovine sperm frozen with low‐density lipoproteins and trehalose

Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

ADIÇÃO DE HIDROXITOLUENO BUTILADO (BHT) NO DILUIDOR ACP-106c PARA CONGELAÇÃO DE SÊMEN CANINO

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