Butterfly pea (Clitoria ternatea) seed and petal extracts decreased HEp‐2 carcinoma cell viability

Abstract

Summary The hydrophilic phenolics, lipophilic tocopherols, phytosterols and fatty acids in butterfly pea seeds and petals were determined. The seeds had fifteen phenolics; of them, sinapic acid, epicatechin and hydroxycinnamic acid derivative concentrations were above 0.5 mg g−1. The petals contained a group of ternatins, flavone glycosides and delphinidin derivatives. Both the seeds and petals had four phytosterols and α‐ and γ‐tocopherols. However, the level of β‐sitosterol or γ‐tocopherol in the seeds was much higher than in the petals. Linoleic acid was the most abundant fatty acid in the seeds and petals, while phytanic acid was found in the petals. The effect of lipophilic and hydrophilic extracts of the seeds [lipophilic extract of the butterfly pea seeds (LBS) and hydrophilic extract of butterfly pea seeds (HBS)] and petals [lipophilic extract of the butterfly pea petals (LBP) and hydrophilic extract of butterfly pea petals (HBP)] on decreased HEp‐2 human carcinoma cell viability was evaluated. The effect of HBS or HBP on decreased cancer cell viability was much higher than that of either LBS or LBP, while HBS showed significantly higher effect than HBP. The results indicated that butterfly pea seed and petal extracts could have the potential in functional food development.

Tinjauan manfaat bunga telang (clitoria ternatea l.) bagi kesehatan manusia

“…Aktivitas anti-proliferatif ekstrak lipofilik dan hidrofilik bunga telang terhadap lini sel kanker laring (Hep-2: human epithelial type 2) dilaporkan oleh (Shen et al, 2016) dengan ekstrak hidrofilik menunjukkan efektifitas yang lebih baik dibandingkan dengan ekstrak lipofilik. Penelitian ini membawa kepada satu perkiraan bahwa fraksi hidrofilik pada bunga telang berperan lebih efektif sebagai antikanker dibandingkan dengan fraksi lipofiliknya.…”

“…Komponen bioaktif lipofilik terdapat dalam jumlah lebih banyak dibandingkan dengan komponen hidrofilik, masing-masing sebesar 27,67 dan 11,08 mg/100 g bunga segar (Shen et al, 2016). Pada fraksi lipofilik, yang paling banyak adalah kelompok fitosterol dan asam lemak.…”

“…Pada fraksi lipofilik, yang paling banyak adalah kelompok fitosterol dan asam lemak. Sementara itu pada fraksi hidrofilik yang terbanyak adalah antosianin dan flavonol glikosida (Kazuma et al, 2003;Shen et al, 2016).…”

“…Hingga saat ini kelompok senyawa terpenoid yang berhasil ditemukan pada bunga telang adalah triterpenoid (yang kemudian diidentifikasi sebagai tarakserol), fitosterol, dan tokoferol (Gambar 5) (Shyam Kumar & Ishwar Bhat, 2011;Shyam Kumar & Ishwar Bhat, 2012;Suganya et al, 2014;Shen et al, 2016;Singh et al, 2018;Zakaria et al, 2018) Secara umum triterpenoid memiliki aktivitas inflamasi, sehingga diperkirakan efek antiinflamasi dan analgesik bunga telang dikontribusikan oleh tarakserol (Shyam Kumar & Ishwar Bhat, 2012).…”

Effects of different solvents on total phenolic and total anthocyanin contents of Clitoria ternatea L. petal and their anti‐cholesterol oxidation capabilities

“…These antioxidant properties are related to an ability to donate hydrogen atoms or electrons to free radicals or to trap and displace free radicals within the aromatic structure (Kaur & Kapoor, 2001). In aqueous CT petal extracts, bioactive compounds such as 3,5-delphinidin glucoside, 3b-delphinidin glucoside, 3b-malvidin glucoside, kaempferol, q-coumaric acid and six types of ternatins: A1, A2, B1, B2, D1 and D2 contribute to antioxidant activity (Chayaratanasin et al, 2015) and anticancer activity (Shen et al, 2016). Therefore, it is necessary to determine the extraction conditions -including pH, soaking time and solvent-typethat give a high yield of bioactive compounds from CT petals, as higher amounts of phenolics typically relate to greater antioxidant capacity (Liu et al, 2017).…”