Butanol fermentation

Schiel-Bengelsdorf, Bettina; Montoya, José Zapata; Linder, Sonja; Dürre, Peter

doi:10.1080/09593330.2013.827746

Cited by 79 publications

(46 citation statements)

References 143 publications

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“…As examples, Clostridium acetobutylicum and Clostridium butyricum are well known to produce acetone-butanol-ethanol (ABE) products during industrial fermentation (3). Clostridium beijerinckii can be used to produce butanol (4), while Clostridium cellulolyticum can use cellulose as a carbon source and generate lactate, acetate, and ethanol as valuable end products (5).…”

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Germinants and Their Receptors in Clostridia

2016

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Many anaerobic spore-forming clostridial species are pathogenic, and some are industrially useful. Although many are strict anaerobes, the bacteria persist under aerobic and growth-limiting conditions as multilayered metabolically dormant spores. For many pathogens, the spore form is what most commonly transmits the organism between hosts. After the spores are introduced into the host, certain proteins (germinant receptors) recognize specific signals (germinants), inducing spores to germinate and subsequently grow into metabolically active cells. Upon germination of the spore into the metabolically active vegetative form, the resulting bacteria can colonize the host and cause disease due to the secretion of toxins from the cell. Spores are resistant to many environmental stressors, which make them challenging to remove from clinical environments. Identifying the conditions and the mechanisms of germination in toxin-producing species could help develop affordable remedies for some infections by inhibiting germination of the spore form. Unrelated to infectious disease, spore formation in species used in the industrial production of chemicals hinders the optimum production of the chemicals due to the depletion of the vegetative cells from the population. Understanding spore germination in acetone-butanol-ethanol-producing species can help boost the production of chemicals, leading to cheaper ethanol-based fuels. Until recently, clostridial spore germination is assumed to be similar to that of Bacillus subtilis. However, recent studies in Clostridium difficile shed light on a mechanism of spore germination that has not been observed in any endospore-forming organisms to date. In this review, we focus on the germinants and the receptors recognizing these germinants in various clostridial species.

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Germinants and Their Receptors in Clostridia

2016

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“…These results revealed that extracellular electron transfer molecules influenced the fermentative metabolism of butanol-producing clostridia on different scales by stimulating or interfering with hydrogen formation. On the other hand, C. acetobutylicum and C. beijerinckii have similar butanol synthetic pathways and regulatory mechanisms (2,3,17). However, C. acetobutylicum grew poorly and produced less solvent than C. beijerinckii using DTH as the carbon source (data not shown).…”

Section: Discussionmentioning

confidence: 90%

“…These genes encoded acetoacetate decarboxylase (adc, Cbei_3835), alcohol dehydrogenase (ald, Cbei_3832), thiolase (thl, Cbei_3630), butyryl-CoA dehydrogenase (bcd, Cbei_0325), nicotinic acid mononucleotide adenylyltransferase (nadD, Cbei_0513), thioredoxin reductase (trxB, Cbei_2681), and Ni/Fe hydrogenase (hyd, Cbei_3013). The adc, ald, thl, and bcd genes are located in the central butanol and acetone synthetic pathway (2,32). The nadD gene is located in the NAD(H) biosynthesis pathway (33), and the hyd gene is involved in hydrogen formation in C. beijerinckii (34).…”

Section: Resultsmentioning

confidence: 99%

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Modulation of the Acetone/Butanol Ratio during Fermentation of Corn Stover-Derived Hydrolysate by Clostridium beijerinckii Strain NCIMB 8052

Liu

Yao

Zhang

et al. 2017

Appl Environ Microbiol

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Producing biobutanol from lignocellulosic biomass has shown promise to ultimately reduce greenhouse gases and alleviate the global energy crisis. However, because of the recalcitrance of a lignocellulosic biomass, a pretreatment of the substrate is needed which in many cases releases soluble lignin compounds (SLCs), which inhibit growth of butanol-producing clostridia. In this study, we found that SLCs changed the acetone/butanol ratio (A/B ratio) during butanol fermentation. The typical A/B molar ratio during Clostridium beijerinckii NCIMB 8052 batch fermentation with glucose as the carbon source is about 0.5. In the present study, the A/B molar ratio during batch fermentation with a lignocellulosic hydrolysate as the carbon source was 0.95 at the end of fermentation. Structural and redox potential changes of the SLCs were characterized before and after fermentation by using gas chromatography/mass spectrometry and electrochemical analyses, which indicated that some exogenous SLCs were involved in distributing electron flow to C. beijerinckii, leading to modulation of the redox balance. This was further demonstrated by the NADH/NAD ϩ ratio and trxB gene expression profile assays at the onset of solventogenic growth. As a result, the A/B ratio of end products changed significantly during C. beijerinckii fermentation using corn stover-derived hydrolysate as the carbon source compared to glucose as the carbon source. These results revealed that SLCs not only inhibited cell growth but also modulated the A/B ratio during C. beijerinckii butanol fermentation.IMPORTANCE Bioconversion of lignocellulosic feedstocks to butanol involves pretreatment, during which hundreds of soluble lignin compounds (SLCs) form. Most of these SLCs inhibit growth of solvent-producing clostridia. However, the mechanism by which these compounds modulate electron flow in clostridia remains elusive. In this study, the results revealed that SLCs changed redox balance by producing oxidative stress and modulating electron flow as electron donors. Production of H 2 and acetone was stimulated, while butanol production remained unchanged, which led to a high A/B ratio during C. beijerinckii fermentation using corn stover-derived hydrolysate as the carbon source. These observations provide insight into utilizing C. beijerinckii to produce butanol from a lignocellulosic biomass.

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“…However, there is now increasing interest in the use of biobutanol as a transport fuel given its many advantages [1]. In fact, a number of companies (such as BP-DuPont and Abengoa) are now investigating novel alternatives to traditional production process, which would enable A C C E P T E D M A N U S C R I P T biobutanol to be produced on an industrial scale [2].…”

Section: Introductionmentioning

confidence: 99%

Phase equilibria of the water+1-butanol+toluene ternary system at 101.3kPa

Gomis

Font

Saquete

et al. 2015

Fluid Phase Equilibria

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Please cite this article as: Vicente Gomis, Alicia Font, María Dolores Saquete, Jorge García-Cano, Phase equilibria of the water + 1-butanol + toluene ternary system at 101.3kPa, Fluid Phase Equilibria http://dx.doi.org/10.1016/j.fluid. 2014.10.038 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Phase equilibria of the water + 1-butanol + toluene ternary system at 101.3 kPa Vicente Gomis 1 , Alicia Font, María Dolores Saquete, Jorge García-Cano University of Alicante. PO Box 99 E-03080 Alicante (Spain) *Corresponding author:Tel. +34 965903400. vgomis@ua.es HighlightsThe phase equilibria of the system water + 1-butanol + toluene have been studied.Isothermal liquid-liquid equilibrium data at 313.15 K was determined.Isobaric vapour-liquid-liquid equilibrium data at 101.3 kPa was also determined.Data obtained has been checked against literature data and those predicted with binary interaction parameters.All experimental data have been correlated with thermodynamic models. AbstractIsobaric vapour-liquid and vapour-liquid-liquid equilibrium data for the water + 1-butanol + toluene ternary system were measured at 101.3 kPa with a modified VLE 602 Fischer apparatus. In addition, the liquid-liquid equilibrium data at 313.15 K were measured and compared with data from other authors at different temperatures. The system exhibits a ternary heterogeneous azeotrope whose temperature and composition have been determined by interpolation. The thermodynamic consistency of the experimental vapour-liquid and vapour-liquid-liquid data was checked by means of the Wisniak's L i /W i consistency test. Moreover, the vapour-liquid and the liquid-liquid equilibrium correlation for the ternary system with NRTL and UNIQUAC models, together with the prediction made with the UNIFAC model, were studied and discussed.

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Butanol fermentation

Cited by 79 publications

References 143 publications

Germinants and Their Receptors in Clostridia

Germinants and Their Receptors in Clostridia

Modulation of the Acetone/Butanol Ratio during Fermentation of Corn Stover-Derived Hydrolysate by Clostridium beijerinckii Strain NCIMB 8052

Phase equilibria of the water+1-butanol+toluene ternary system at 101.3kPa

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