This study aims to study the productivity and quality of Isa Brown chicken eggs given tuna fish oil in the ration. The design used was a completely randomized design (CRD) with 200 chickens with 4 treatments and 5 replications and each replication consisted of 10 chickens. P0 : Ration without the use of fish oil, P1 : Ration with the use of 0.25 % fish oil, P2 : The ration with the use of 0.50% fish oil, P3 : The ration with the use of 0.75% fish oil. The feed used is composed of feed ingredients, namely corn, bran, and KLS Super Plus. The variables observed included daily egg production, ration consumption, egg weight, egg index, egg pH, HU (haugh unit), egg yolk color, FCR (Feed Conversion Ratio), cholesterol in eggs. The results showed that the use of fish oil of 0.25%, 0.50%, 0.75% in the ration gave significant results (P<0.05) on ration consumption, egg weight, egg pH, egg yolk color. The results were not significantly different (P>0.05) in daily egg production, egg index, HU (Haugh Unit), FCR (Feed Conversion Ratio), and egg cholesterol.