Buruli Ulcer: A Review of<i>In Vitro</i>Tests to Screen Natural Products for Activity against<i>Mycobacterium ulcerans</i>

Yemoa, Achille; Gbénou, Joachim; Affolabi, Dissou; Moudachirou, Mansourou; Bigot, André; Anagonou, S.; Portaels, Françoise; Quetin-Leclercq, Joëlle; Martin, Anandi

doi:10.1055/s-0030-1250642

Cited by 29 publications

(30 citation statements)

References 38 publications

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“…7,17 Buruli ulcer is known to affect poor, rural communities. 9,11,[19][20][21][22] Only 19% of our respondents received a regular income, 26.0% were unskilled workers, mainly subsistence farmers or petty traders, and most (55.3%) were in school.…”

Section: Discussionmentioning

confidence: 83%

Help-Seeking for Pre-Ulcer and Ulcer Conditions of Mycobacterium ulcerans Disease (Buruli Ulcer) in Ghana

Ackumey

Gyapong²,

Pappoe³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. This study examined sociocultural features of help-seeking for Buruli ulcer-affected persons with pre-ulcers and ulcers in a disease-endemic area in Ghana. A sample of 181 respondents were purposively selected. Fisher's exact test was used to compare help-seeking variables for pre-ulcers and ulcers. Qualitative phenomenologic analysis of narratives clarified the meaning and content of selected quantitative help-seeking variables. For pre-ulcers, herbal dressings were used to expose necrotic tissues and subsequently applied as dressings for ulcers. Analgesics and left-over antibiotics were used to ease pain and reduce inflammation. Choices for outside-help were influenced by the perceived effectiveness of the treatment, the closeness of the provider to residences, and family and friends. Health education is required to emphasize the risk of self-medication with antibiotics and the importance of medical treatment for pre-ulcers, and to caution against the use of herbs to expose necrotic tissues, which could lead to co-infections.

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Section: Discussionmentioning

confidence: 83%

Help-Seeking for Pre-Ulcer and Ulcer Conditions of Mycobacterium ulcerans Disease (Buruli Ulcer) in Ghana

Ackumey

Gyapong²,

Pappoe³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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“…macrocarpus, M. cabraei, C. spectabilis, C. medica, M. villosus, T. superba, C. pentandra et I. aquatica. Parmi les seize autres espèces, indiquées par un seul informateur, trois plantes sont indiquées dans le traitement traditionnel de l'UB en Afrique de l'Ouest, en l'occurrence Aframomum melegueta au Benin et au Ghana (Yemoa et al, 2008(Yemoa et al, , 2011Seefeld et al, 2013); Elaeis guineensis en Côte d'Ivoire (Adjet et al, 2013 ;; Spathodea campanulata au Benin, Côte d'Ivoire et Ghana (Adjet et al, 2013;Sam et al, 2013;Addo et al, 2007;Yemoa et al, 2008;. Les treize autres espèces, non indiquées dans le littérature comme plantes anti-ulcère de Buruli sont plutôt indiquées pour d'autres indications thérapeutiques telles que le diabète, la jaunisse, la gale, les céphalées et fièvres, la tuberculose, les dermatoses, l'ascite, l'épilepsie, les éruptions cutanées, la blennorragie, les vers intestinaux, l'hypertension artérielle, la typhoïde (Dongmo et al, 2007;N'guessan et al, 2009;Mangambu et al, 2014;.…”

Section: Discussionunclassified

“…Des travaux ont été entrepris pour mieux étudier ces plantes et thérapies traditionnelles afin de disposer des informations par rapport à l'efficacité et/ou l'innocuité de ces dernières. Plusieurs espèces de plantes médicinales traditionnellement utilisées pour traiter l'UB ont été identifiées, leur composition chimique définie et leurs activités antibactériennes sur Mycobacterium ulcerans, ainsi que leur toxicité déterminées en Afrique de l'Ouest de manière globale (Tsouh Fokou et al, 2015, et plus spécifiquement au Benin (Yemoa et al, 2008(Yemoa et al, , 2011(Yemoa et al, , 2015, au Ghana (Addo et al, 2007), en Côte d'Ivoire (Coulibaly et al, 2011). Au Cameroun, les travaux de Pouillot et al (2007), Landier et al (2011) soit par massage et désinfection de la lésion avec les décoctions préparées (64,3%), soit par application locale en cataplasme (14,3%) ou par badigeonnage (3,6%), soit par projection sur les lésions ou plaies ulcéreuses, de la préparation contenue dans des fragments de feuilles de bananier préalablement ramollies au feu de bois (14,3%).…”

Section: Introductionunclassified

Approche ethnobotanique et ethnopharmacologique des plantes utilisées dans le traitement traditionnel de l’ulcère de Buruli à Akonolinga (Cameroun)

Herve¹,

Guedje²,

Biye³

2017

Int. J. Bio. Chem. Sci

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Ethnopharmacological and ethnobotanical approach of medicinal plants used in the traditional treatment of Buruli ulcer in Akonolinga (Cameroon) ABSTRACTFew data are available concerning plants used in the traditional treatment of Buruli ulcer in Cameroon. This study aimed to identify species and characterize anti-Buruli ulcer recipes in the Akonolinga district. The H. N. BAYAGA et al. / Int. J. Biol. Chem. Sci. 11(4): 1523-1541, 2017 1524 ethnopharmacological survey has enabled to identify 25 plant species used against Buruli ulcer. The plant parts mostly used were stem bark (41.1%), stem (26.8%) and leaves (25%). Decoction (64.3%) and pounding (23.2%), the most dominant preparing method, were administrated externally, mostly by massage and disinfection (64.3%) and by local poultice application (14.3%). The analysis of relative frequency of citation, as well as the search of similarity and convergence of use, have shown that Musa parasidiaca, Mitracarpus villosus, Aframomum melegueta, Elaeis guineensis and Spathodea campanulata were among species exhibiting remarkable convergence of use between different geographical regions; while seven others, Petersianthus macrocarpus, Momordica cabraei, Cassia spectabilis, Citrus medica, Terminalia superba, Ceiba pentandra and Ipomoea aquatica, formed another most significant species group. In highlighting those 12 species of interest, this study has direct bearing in drug design and innovation by the ethnopharmacological reasoning, thereby contributing to species selection and direction for prior chemical, pharmacological and clinical assessments leading to plant-based drug development that Africa needed for its pandemic pathologies.

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“…The incubation period varies according to the type of culture medium used and is generally longer on solid medium. Cultures are read within 2 to 3 weeks with Middlebrook 7H12, 4 to 8 weeks with Middlebrook 7H11 agar, and 6 to 8 weeks or more with LJ while 8 to 14 days are required when Middlebrook 7H9 broth is used (Scherr, Röltgen, Witschel, & Pluschke, 2012;Yemoa et al, 2011).…”

Section: Growth Conditionsmentioning

confidence: 99%

“…The turbidity of the resulting suspension is then adjusted with distilled water to be equivalent to that of a standard 1 mg/ml suspension of M. bovis BCG (containing approximately 10 8 CFU per ml), from which further dilution is performed to 10 −1 and 10 −2 mg/ml. The inocula are 0.1 ml of diluted solutions (Yemoa et al, 2011). Therefore in preparing bacteria suspension for susceptibility testing, M. ulcerans growth patterns such as slow growing and a low optimum growth temperature between 28 and 32°C; as well as ability to aggregate in broth, should be considered in designing a suitable testing system.…”

Section: Bacterial Loadmentioning

confidence: 99%

Methods used in preclinical assessment of anti-Buruli ulcer agents: A global perspective

Tsouh

Addo

Yeboah-Manu

et al. 2015

Journal of Pharmacological and Toxicological Methods

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Buruli ulcer (BU) caused by Mycobacterium ulcerans is the third most common chronic mycobacterial infection in humans. Approximately 5000 cases are reported annually from at least 33 countries around the globe, especially in rural African communities. Even though anti-mycobacterial therapy is often effective for early nodular or ulcerative lesions, surgery is sometimes employed for aiding wound healing and correction of deformities. The usefulness of the antibiotherapy nonetheless is challenged by huge restrictive factors such as high cost, surgical scars and loss of income due to loss of man-hours, and in some instances employment. For these reasons, more effective and safer drugs are urgently needed, and research programs into alternative therapeutics including investigation of natural products should be encouraged. There is the need for appropriate susceptibility testing methods for the evaluation of potency. A number of biological assay methodologies are in current use, ranging from the classical agar and broth dilution assay formats, to radiorespirometric, dye-based, and fluorescent/luminescence reporter assays. Mice, rats, armadillo, guinea pigs, monkeys, grass cutters and lizards have been suggested as animal models for Buruli ulcer. This review presents an overview of in vitro and in vivo susceptibility testing methods developed so far for the determination of anti-Buruli ulcer activity of natural products and derivatives.

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Buruli Ulcer: A Review ofIn VitroTests to Screen Natural Products for Activity againstMycobacterium ulcerans

Cited by 29 publications

References 38 publications

Help-Seeking for Pre-Ulcer and Ulcer Conditions of Mycobacterium ulcerans Disease (Buruli Ulcer) in Ghana

Help-Seeking for Pre-Ulcer and Ulcer Conditions of Mycobacterium ulcerans Disease (Buruli Ulcer) in Ghana

Approche ethnobotanique et ethnopharmacologique des plantes utilisées dans le traitement traditionnel de l’ulcère de Buruli à Akonolinga (Cameroun)

Methods used in preclinical assessment of anti-Buruli ulcer agents: A global perspective

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