Currently, many pharmacists suffer from burnout as a result of high demand for their services, lack of resources, shortage of pharmacy staff. No studies in Saudi Arabia among hospital pharmacists have focused on burnout. Therefore, this study was conducted to assess the prevalence of burnout among pharmacists at the National Guard Hospital (NGHA).A cross-sectional study was conducted at National Guard Hospital (NGHA) Riyadh, Saudi Arabia. Data were collected using a questionnaire through Google form by a smart device. The questionnaire consists of two sections: the first section is demographic data while the second section is the Maslach Burnout Inventory for Health Services Workers (MBI-HSS). Participants were selected by the non-probability consecutive sampling method. They were 159 full-time employees working in the hospital, including clinical pharmacists, pharmacy residents, general pharmacists, and pharmacy technicians. Forty employees (25.16%) had high emotional exhaustion, 89 (55.97%) had high depersonalization, and 101 (63.52%) had low scores for personal accomplishment. The common criteria are high emotional exhaustion, high depersonalization, and low personal accomplishment which indicated pharmacists with burnout (17.6%). Roughly, a quarter of the National Guard Hospital pharmacists have burnout. A correlation between personal accomplishment, gender, and the number of exercises per week is significant. Pharmacy employee satisfaction with their income, age and the number of sleeping hours per day was inversely correlated with emotional exhaustion and depersonalization level.