Background/Aim. Professional military personnel are exposed to a number of stressors during the war as well as in peacetime conditions that can cause some hidden or manifest disorders, especially anxiety and depression, but also the development of a burnout syndrome. The aim of our investigation was to determine the defense mechanisms and subjective assessment of quality of life and anxiety in professional military personnel of the Serbian Armed Forces with the burnout syndrome. Methods. The cross-sectional study included a total of 55 professional military personnel, from 25 to 55 years of age, without current mental problem. In the investigation, the Maslach Burnout Inventory (MBI), Defense Style Questionnaire (DSQ-40), World Health Organization Abbreviated Instrument for Quality of Life Assessment (WHOQOL-BREF) and Beck Anxiety Inventory were used. The statistical analysis included parametric and nonparametric descriptive statistics. Results. Emotional exhaustion and depersonalization were present in 10.9% of subjects and in 12.7% of subjects respectively, in moderate se može uticati na smanjenje anksioznosti i poboljšanje kvaliteta života. U težim slučajevima je potrebna i stručna pomoć, uključujući i psihoterapiju.