Burn injury induces histopathological changes and cell proliferation in liver of rats

Bortolin, Jeferson André; Quintana, Hananiah Tardivo; Tomé, Tabata de Carvalho; Ribeiro, Flávia Andressa Pidone; Ribeiro, Daniel Araki; Oliveira, Flávia de

doi:10.4254/wjh.v8.i6.322

Cited by 20 publications

(13 citation statements)

References 28 publications

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“…Walker and Mason [ 6 ] used three different lengths of time – 3, 5 and 10 seconds – to obtain three types of injury depth. Two studies 14 , 16 used 3 seconds and observed a superficial (first degree) burn, which is consistent with the original study. Canhão et al 15 .…”

Section: Discussionsupporting

confidence: 77%

“…Walker and Mason 6 used 100°C as water temperature and 4 studies 18 , 19 , 22 , 25 did the same. More recent studies 16 , 17 , 23 used 90°C to 98°C as water temperatures, yet, the results were the same as those using 100°C; 2 studies [ 17 , 21 ] used 70°C to 78°C so as to obtain a more superficial injury; and 3 studies 14 , 15 , 17 used 80°C a 89°C and observed a median injury. The sum of the water temperature and the length of exposure time to the water are important to determine the degree of the injury.…”

Section: Discussionmentioning

confidence: 95%

“…used 7 to 9 seconds to obtain a median injury (second degree); however, Walker and Mason 6 observed the same result using 4 seconds. In order to observe a full thickness burn (third degree), 7 studies used the same amount of seconds as Walker and Mason, i.e., 10 seconds 14 , 16 , 17 , 19 , 22 , 23 , 25 . Vana et al 20 .…”

Section: Discussionmentioning

confidence: 99%

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Experimental models of scald burns. A scope review

et al. 2019

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Purpose:To conduct a scope review of the experimental model described by Walker and Mason, by identifying and analyzing the details of the method.Methods:The authors searched Pubmed-Medline, Cochrane-Bireme and PEDro databases for articles published between January 2016 and December 2018, using the following search queries: burns, burn injuries, models animal, and animal experimentation. All articles whose authors used Walker and Mason's model - with or without changes to the method in Wistar rats - were included in this study.Results:The search identified 45 mentions of Walker and Mason's model; however, after reading each summary, 20 were excluded (of which 5 due to duplicity). The inconsistencies observed after the scope review were: water temperature, length of time of exposure of the experimental model's skin to water, extent of the burnt area, and the description of the thickness/depth of the injury.Conclusions:Reproducibility of a scientific method is the basis to prove the veracity of the observed results. Thus, it is necessary to have a greater number of publications that adopt a reproducible scientific method, for this review found inconsistencies in the description of Walker and Mason's model.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 95%

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Experimental models of scald burns. A scope review

et al. 2019

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“…However, the feasibility of megalocyte as a precancerous cell is not confirmed although the study of liver carcinogenesis in Wistar rats with a single dose of 7 mg/kg AFB 1 or subsequently repeated CCl 4 inhalation demonstrates that megalocytosis is the first observed lesion and the tumor cells usually located among the megalocytic cells [38]. In another aspect, polyploidy, which is usually found as megalocyte and binucleated cell, could also denote an adaptive mechanism to become more resistant to oxidative stress and genotoxic damage from xenobiotic [4, 14, 17, 28]. Several researchers elucidated that diploid hepatocytes enter the cell cycle faster than polyploid cells.…”

Section: Discussionmentioning

confidence: 99%

The effects of astaxanthin on liver histopathology and expression of superoxide dismutase in rat aflatoxicosis

Monmeesil

Fungfuang

Tulayakul

et al. 2019

The Journal of Veterinary Medical Science

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The metabolism of aflatoxin B 1 (AFB 1 ) generates reactive oxygen species (ROS) that destroys hepatocytes. Meanwhile, astaxanthin (AX) is known to have stronger antioxidative activity than other carotenoids. This study aimed to investigate hepatoprotective role of AX from AFB 1 -induced toxicity in rat by histopathological study and immunohistochemistry of Cu/Zn-SOD (SOD1) which acts as the first enzyme in antioxidative reaction against cell injury from ROS. Twenty Wistar rats were randomly divided into 4 groups. The control and AFB 1 groups were gavaged by water for 7 days followed by a single DMSO and 1 mg/kg AFB 1 , respectively. The AXL+ AFB 1 and AXH+ AFB 1 groups were given of 5 mg/kg and 100 mg/kg AX for 7 days before 1 mg/kg AFB 1 administration. The result showed significantly elevated liver weight per 100 g body weight in AFB 1 group. The histopathological finding revealed vacuolar degeneration, necrosis, megalocytosis and binucleation of hepatocytes with bile duct hyperplasia in AFB 1 group. The severities of pathological changes were sequentially reduced in AXL+AFB 1 and AXH+AFB 1 groups. Most rats in AXH+AFB 1 group owned hypertrophic hepatocytes and atypical proliferation of cholangiocytes which are adaptive responses to severe hepatocyte damage. The SOD1 expression was also significantly higher in AXH+AFB 1 group than solely treated AFB 1 and AXL+AFB 1 groups. In conclusion, AX alleviated AFB 1 -induced liver damage in rat by stimulating SOD1 expression and transdifferentiation of cholangiocytes in dose dependent manner.

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“…Some hepatocytes showed pyknotic nucleus or karyolysis. In context, Bortolin et al [23] recorded binucleation as consequence of cell injury followed by regenerative growth after injury [23] .…”

Section: Discussionmentioning

confidence: 99%

Histological and immunohistochemical study of the effect of omega-3 fatty acids on ifosfamide-induced liver injury in adult male albino rat

Kalawy

Mostafa

Fattah

et al. 2017

Journal of Medical Histology

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Background and Objectives: GIfosfamide (IFO) is a highly effective chemotherapeutic agent used for treating a variety of cancer but has reported to cause certain side effects such as hepatotoxicity. Being antioxidant and anti-inflammatory agents, omega-3 fatty acids (ω3 FAs) were effective in the treatment of various medical conditions. This study aimed to elucidate the possible hepatoprotective role of ω3 FAs against IFO-induced liver injury. Methods and Results: A total of 42 adult male albino rats were divided into 4 groups; the control group (Gp I) of 12 rats, and 3 groups each of 10 rats. Omega-3 group (Gp II) received 300 mg/kg/day of omega-3 plus orally. IFO group (Gp III) received intraperitoneal injection of IFO 50 mg/kg, which was subdivided equally into IFO subgroup (Gp IIIa) and IFO recovery subgroup (Gp IIIb). IFO + Omega-3 group (Gp IV) received omega-3 plus as pretreatment and combined treatment with IFO. Liver sections were stained with Hematoxylin and Eosin (H&E), Periodic acid Schiff (PAS) and immunohistochemical stain for caspase-3. The mean area percent (%) of glycogen & optical density of immunopositive cells were measured. IFO had degenerative effects on rat hepatocytes with marked depletion of glycogen, inflammatory cells infiltration, dilatation and congestion of blood sinusoids, prominent Kupffer cells (KC), besides, a significant increase in caspase-3 immunoexpression. However, these alterations were far less pronounced in IFO + Omega-3 group. Conclusions: Administration of omega-3 FAs as a pretreatment and combined treatment with IFO ameliorated the liver injury induced by IFO indicating its hepatoprotective effects.

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Burn injury induces histopathological changes and cell proliferation in liver of rats

Abstract: BI induces histopathological changes, upregulation of COX-2 immunoexpression, and cell proliferation in liver of rats.

Cited by 20 publications

References 28 publications

Experimental models of scald burns. A scope review

Experimental models of scald burns. A scope review

The effects of astaxanthin on liver histopathology and expression of superoxide dismutase in rat aflatoxicosis

Histological and immunohistochemical study of the effect of omega-3 fatty acids on ifosfamide-induced liver injury in adult male albino rat

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