Burkitt's lymphoma cells: Membrane properties and surface morphology as seen by scanning electron microscopy

Polliack, Aaron; Yefenof, Eitan; Gamliel, Haim; Klein, George

doi:10.1016/0145-2126(79)90045-6

Cited by 9 publications

(3 citation statements)

References 21 publications

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“…We confirm here the reported presence of surface pores. However, we are inclined to regard the occurrence of blebs as a taxonomic criterion with some degree of suspicion; particularly when we recall the lability of the surface microvilli of lymphocytes, for example, and their sensitivity to preparative procedures [13]. Intensity of surface ruffling may be related to progress of a megakaryocyte towards platelet release, much as it is an expression of hyperactivity in macrophages [ 141 .…”

Section: Discussionmentioning

confidence: 99%

Aspects of platelet formation and release

Scurfield

Radley

1981

American J Hematol

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The surface architecture and location of megakaryocytes in the extravascular compartment of mouse bone marrow are considered. The finger-like cytoplasmic processes that extend into sinuses pass through apertures, approximately 3.0 millimicron in diameter, occurring singly or in groups. The cytoplasmic processes enlarge on first entering a sinus and form villi that seem to anchor them to endothelium. The villi consist largely of microfilaments. The cytoplasmic processes extend, attenuate, and undergo irregular constriction along their lengths. The considerations demarcate modicums of cytoplasm of platelet size one from another that are released when they break. Extravascular platelet release may also occur, and evidence is presented that points to the likelihood of the platelet's being engulfed and phagocytosed by macrophages before reaching the microcirculation.

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Section: Discussionmentioning

confidence: 99%

Aspects of platelet formation and release

Scurfield

Radley

1981

American J Hematol

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“…3) (Polliack et al, 1973b(Polliack et al, , 1976aLevine & Polliack, 1975). At the time it was not obvious, despite protesting studies by Wetzel et al (1973,1974), that lymphocyte microvilli, may be labile structures which could appear and retract in response to a host of internal and microenvironmental stimuli such as temperature (Lin et al, 1973a), cell cycle (Porter et al, 1973), intercellular contact (Rubin & Everhart, 1973), maturation and antigenic stimulation (Baur et al, 1975;Polliack et al, 1980a) or even to variations in preparatory techniques (Wetzel et al, 1974, Barber & Burkholder, 1975.…”

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confidence: 99%

The contribution of scanning electron microscopy in haematology: its role in defining leucocyte and erythrocyte disorders

Polliack¹

1981

Journal of Microscopy

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SUMMARY This report reviews the contribution of scanning electron microscopy (SEM) in haematology. Important observations regarding red cell shape and deformities are referred to and contributions in the definition and classification erythrocyte disorders are stressed. In this field there is no doubt that SEM has contributed much to the three‐dimensional visualization of RBC disorders. As far as leucocyte pathology is concerned the situation is less clear. SEM has contributed much to current knowledge and understanding of the surface properties of white blood cells. Normal leucocytes have different surface features and can be distinguished under the SEM. However, some overlap does occur, making individual distinction on the basis of surface architecture alone extremely difficult. The difficulties in this regard are discussed in this review and factors influencing the variability of surface microprojections are reviewed briefly. Leukaemic cells of different origins may also be distinguished under the SEM. However, while ‘hairy’ cells have typical surface features and non‐lymphoid leukaemic cells, in particular monocytes, are readily distinguished from lymphoid leukaemic cells, there is much overlap of surface topography. Undifferentiated cells and early myeloblasts and lymphoblasts have similar surface features and cannot be distinguished under the SEM. While SEM adds a valuable third dimension to morphology and ultrastructure, it cannot be used alone in the definition of difficult cases of acute leukaemia.

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“…Cultured cells from the following established cell lines were also studied: Raji and Daudi Burkitt's lymphoma (Ben Bassat et al, 1977;Polliack et al, 1979); HD-Mar and Molt 4 (Ben Bassat et al, 1977, both malignant T cell lines; the K-562 cell line (Klein et al, 1976), derived from a patient with blastic crisis of CGL but now known to be erythroid in nature (Anderson et al, 1979); Reh null type ALL line (Polliack et al, 1981b); NALM-I, null type ALL line now known to be of pre-B origin (Minowada et al, 1979); ANRF, a cloned cell line of EBV transformed lymphocytes, derived from a patient with rheumatoid arthritis, producing monoclonal rheumatoid factor (Steinitz et al, 1980); Mouse-VIP, T cell lymphoma induced by radiation leukaemia virus in a C-57 black mouse (Yefenof et al, 1980). These cell lines were kindly provided by Drs H. Ben Bassat, M. Steinitz and E. Yefenof (Jerusalem), Dr J. Minowada (New York) and Drs E. Thiel and H. Rodt (Munich).…”

Section: A T E R I a L S And M E T H O D Smentioning

confidence: 99%

Scanning immuno‐electron microscopy of human leukaemia and lymphoma cells: a comparative study of techniques using immunolatex spheres as markers

Gamliel

Leizerowitz

Gurfel

et al. 1981

Journal of Microscopy

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In this study scanning immuno-electron microscopic (SIEM) techniques were used to identify human leukaemia-lymphoma cells. Monodispersed polystyrene (latex) beads were conjugated to specific antisera using glutaraldehyde, in an attempt to detect surface antigenic components on a variety of cells of known origin. Antisera, mostly immunoglobulin fractions, against human thymus (T) derived cells, common type acute lymphoblastic leukaemia cells (C/ALL) and surface immunoglobulin (sIg) bearing cells were used to coat latex spheres, while rabbit anti-mouse Thy-1 antiserum or whole human-IgG (y-globulin) bound to latex were used as controls in some experiments. The use of SIEM techniques in the direct mode as a simple and sensitive method for labelling surface antigens is described. The disadvantages of the SIEM methodology are also summarized while the requirements for optimal cell preparation using this technique are stressed. The experiments were designed to ascertain whether prolonged fixation of cells could be used prior to incubation of the cells with the marker. In this respect, repeated neutralization of the glutaraldehyde with glycine is essential. SIEM labelling of cells is random and unreliable without adequate quenching with glycine. The heteroantisera used in this study proved to be adequate and insignificant non-specific attachment and cross reactivity were seen. SIEM adds a further dimension to ultrastructural aspects of immunology and is a potentially useful tool in the study and identification of leukaemia and lymphoma cells.

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Burkitt's lymphoma cells: Membrane properties and surface morphology as seen by scanning electron microscopy

Cited by 9 publications

References 21 publications

Aspects of platelet formation and release

Aspects of platelet formation and release

The contribution of scanning electron microscopy in haematology: its role in defining leucocyte and erythrocyte disorders

Scanning immuno‐electron microscopy of human leukaemia and lymphoma cells: a comparative study of techniques using immunolatex spheres as markers

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