Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources

Abstract

Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Boo L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9%, respectively) and towards established Bcc species (4.0 and 3.9%, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156(T) (=CCUG 65686(T)) and Burkholderia territorii sp. nov. with type strain LMG 28158(T) (=CCUG 65687(T)) are proposed for Bcc B and Bcc L bacteria, respectively

Incidence of Burkholderia contaminans at a cystic fibrosis centre with an unusually high representation of Burkholderia cepacia during 15 years of epidemiological surveillance

“…In the last few years, the taxonomy of the Bcc has evolved significantly, with the description of novel species (Yabuuchi et al, 2000;Coenye et al, 2001;Vandamme et al, 2002;Vermis et al, 2002Vermis et al, , 2004LiPuma, 2005;Vanlaere et al, 2008Vanlaere et al, , 2009Peeters et al, 2013;Smet et al, 2015). Multicenter studies conducted up to 2009 revealed the existence of several clusters of isolates that do not group with the Bcc species described at the time.…”

Transfer of eleven species of the genus Burkholderia to the genus Paraburkholderia and proposal of Caballeronia gen. nov. to accommodate twelve species of the genera Burkholderia and Paraburkholderia

“…In the period between the effective and valid publications of these species names, 16 novel species of the genus Burkholderia were described. The 16S rRNA gene sequences of two of them, Burkholderia stagnalis and Burkholderia territorii, are most similar to the 16S rRNA gene sequence of Burkholderia glumae, and the nearest neighbours of these novel species in a phylogenetic tree based on concatenated sequences of seven housekeeping gene fragments are Burkholderia ubonensis and Burkholderia latens (De Smet et al, 2015). However, analysis of the taxonomic positions of the other 14 species shows that they need to be reconsidered in accordance with the reclassification of species of the genus Burkholderia proposed by Sawana et al (2014).…”

Biofilms produced by Burkholderia cenocepacia: influence of media and solid supports on composition of matrix exopolysaccharides

“…In particular, the bronchopulmonary epithelium is covered with a hyperviscous mucus, which impairs mucociliary clearance and favours bacterial colonization and infection. Of the 20 species that currently make up the BCC (Mahenthiralingam et al, 2000;Coenye et al, 2003;Peeters et al, 2013;De Smet et al, 2015), all can cause CF infection, with B. cenocepacia being an important CF pathogen, generally more aggressive than other BCC species. When BCC infects CF patients, the clinical outcome is uncertain, with some patients being colonized for years before showing adverse symptoms and others quickly experiencing diminished respiratory capacity and dying of 'cepacia syndrome' (Govan & Deretic, 1996).…”