Burkholderia sp. from rhizosphere of Rhododendron arboretum: Isolation, identification and plant growth promotory (PGP) activities

Abstract

Plant growth promoting rhizobacteria (PGPR) is beneficial bacteria that colonize plant roots and enhance plant growth by wide variety of mechanism like phosphate solubilisation, etc. Use of PGPR has steadily increased in agriculture and offers an attractive way to replace chemical fertilizers, pesticides and supplements. The present research work was designed to isolate and characterize the PGP activity of Burkholderia sp. For this purpose rhizospheric soil from Rhododendron arboreum of Kumaun Himalaya was collected and efficient bacterial strain was screened on the basis of phosphate solubilization. Further, assessment of various parameters of plant growth promotion activity was done and enhanced production of IAA (16.4 µgml ) was observed in the presence of 250µgml -1 and 500 µg ml -1 of tryptophan, respectively. Correspondingly, in respect of 7.8 µg ml -1 IAA without tryptophan, and their confirmation was executed by TLC. A remarkable change in color from green to reddish-brown zone on CAS plates, suggests the positive result for siderophore production, and finally the seed germination and pot trial experiment depicted the growth index of wheat plant. Therefore, the present study suggests that Burkholderia sp. is beneficial for plant growth promotion.

Screening of Phosphate Solubilizing Microorganisms Collected from Post Tin Mining: Potency for Future Mining Rehabilitation

“…The periplasmic space produces organic acids through the direct oxidation pathway (Zhao et al, 2014). Filamentous fungi may increase P solubilization by producing organic acid (Li et al, 2016) and siderophore (Karmakar et al, 2018), whereas bacteria may produce enzyme phosphatase (Abdelgalil et al, 2022), organic acid (Chen et al, 2016), and siderophore (Nailwal et al, 2014).…”

Siderophore Production by Rhizosphere Inhabiting Bacteria and Fungi

“…In both methods, the CAS agar medium containing the blue dye complex of CAS, Fe +3 and HDTMA, the colour change from blue into orange or dark purplish-red color indicates the Fe-binding compound production. In this study, CAS Indicator Solution and the basal agar medium was prepared as (Nailwal et al, 2014). During the preparation of CAS dye PIPES buffer was replaced with (0.1 M) HEPES buffer as a modification described in 2006 (Mirabello, 2006).…”