Burkholderia sacchari (synonym Paraburkholderia sacchari): An industrial and versatile bacterial chassis for sustainable biosynthesis of polyhydroxyalkanoates and other bioproducts

Oliveira-Filho, Edmar R.; Gomez, José Gregório Cabrera; Taciro, Marilda Keico; Silva, Luiziana Ferreira da

doi:10.1016/j.biortech.2021.125472

Cited by 19 publications

(3 citation statements)

References 119 publications

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“…In contrast to the well-studied P(3HB- co -3HV) two-component copolymers, which are synthesized pure cultures of microorganisms, for example C. necator or Burkholderia sacchari , use a variety of C-substrates and precursor compounds [ 37 , 38 ] or mixed microbial cultures (MMC) [ 39 , 40 ]. Information on the conditions for the synthesis of P(3HB- co -3HV- co -4HV) three-component copolymers is very limited ( Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

et al. 2022

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Synthesis of P(3HB-co-3HV-co-4HV) copolymers by the wild-type strain Cupriavidus necator B-10646 on fructose or sodium butyrate as the main C-substrate with the addition of γ-valerolactone as a precursor of 3HV and 4HV monomers was studied. Bacterial cells were cultivated in the modes that enabled production of a series of copolymers with molar fractions of 3HV (from 7.3 to 23.4 mol.%) and 4HV (from 1.9 to 4.7 mol.%) with bacterial biomass concentration (8.2 ± 0.2 g/L) and PHA content (80 ± 2%). Using HPLC, DTA,DSC, X-Ray, SEM, and AFM, the physicochemical properties of copolymers and films prepared from them have been investigated as dependent on proportions of monomers. Copolymers are characterized by a reduced degree of crystallinity (Cx 38–49%) molecular weight characteristics Mn (45–87 kDa), and Mw (201–248 kDa) compared with P(3HB). The properties of the films surface of various composition including the porosity and surface roughness were studied. Most of the samples showed a decrease in the average pore area and an increase in their number with a total increase in 3HV and 4HV monomers. The results allow scaling up the productive synthesis of P(3HB-co-3HV-co-4HV) copolymers using Cupriavidus necator B-10646.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

et al. 2022

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“…B. sacchari contained 62 % of its biomass as PHA when grown on sugarcane bagasse in an integrated biorefinery, while the engineered strain had an augmented yield of 80 % [ 185 ]). PHA is also produced by recombinant E. coli harboring on molasses and sucrose [ 186 ].…”

Section: Bacterial Biorefinerymentioning

confidence: 99%

Application of microbial resources in biorefineries: Current trend and future prospects

Gaur,

Kaur,

Kalra

et al. 2024

Heliyon

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“…In many cases, hydrolysis of said polysaccharides is not even needed; a cohort of strains is reported to directly convert di-, oligo-, and polysaccharides to biomass and PHAs, provided the strains have the adequate hydrolase enzymes available. Examples include the expedient direct starch converter Haloferax mediterranei, which has high amylolytic activity [34]; the invertase-excreting strain Paraburkholderia sacchari [35], which readily converts the disaccharide sucrose; or Hydrogenophaga pseudopflava, a well-described consumer of the disaccharide lactose [36]. Other strains are excellent PHA accumulators from lipids produced by natural organisms (microbes, plants, or animals), such as Pseudomonas putida [37].…”

Section: Pha Biosynthesis Is Based On Natural Feedstocksmentioning

confidence: 99%

Microbial PolyHydroxyAlkanoate (PHA) Biopolymers – Intrinsically Natural

Mukherjee¹,

Koller²

2023

Preprint

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Global pollution from fossil plastics is one of the top environmental threats of our times. The end-of-life scenarios of fossil plastic, including recycling, incineration and disposal result in mi-croplastic formation, elevated atmospheric CO2 levels, and littering of terrestrial and aquatic environments by plastic waste. Currently thought-out regulations centered around banning plastics production and use and only recycling on a regional, national and global level are impeding efforts to rapidly replace fossil plastics through the use of natural alternatives. In particular, this review demonstrates how mi-crobial polyhydroxyalkanoates (PHA), a class of intrinsically natural polymers, can easily remedy for the fossil and persistent plastic dilemma. PHA are bio-based, bio-synthesized, bio-compatible, bio-degradable, and home- and industrially compostable. Therefore, they are one of the perfect replacements for our fossil plastics pollution dilemma, providing us with the benefits of fossil plastics and meeting all requirements of a truly circular economy. PHA biopolyesters are natural and green materials in all aspects of their life cycle. The review elaborates in detail how PHA’s production, consumption, and end-of-life profile are perfectly embedded in the current topical 12 Principles of Green Chemistry, which constitute the basis for sustainable product manufacturing. It is shown that it is time for a paradigm shift in plastics manufacturing, use and disposal. Hu-mankind needs alternatives to fossil plastics, which, as recalcitrant xenobiotics, contribute to the increasing deterioration of our planet. Natural PHA biopolyesters represent that paradigm shift!

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Burkholderia sacchari (synonym Paraburkholderia sacchari): An industrial and versatile bacterial chassis for sustainable biosynthesis of polyhydroxyalkanoates and other bioproducts

Cited by 19 publications

References 119 publications

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

Application of microbial resources in biorefineries: Current trend and future prospects

Microbial PolyHydroxyAlkanoate (PHA) Biopolymers – Intrinsically Natural

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