Burkholderia pseudomallei Antibodies in Individuals Living in Endemic Regions in Northeastern Brazil

Rolim, Dionne Bezerra; Vilar, Dina Cortez Feitosa Lima; Cavalcanti, Luciano Pamplona de Góes; Freitas, Liara B. N.; Inglis, Timothy J. J.; Rodrigues, Jorge Luíz Nobre; Nagão-Dias, Aparecida Tiemi

doi:10.4269/ajtmh.2011.10-0220

Cited by 16 publications

(17 citation statements)

References 19 publications

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“…Sera from melioidosis patients contain predominantly IgG1 and IgG2, which react with culture filtrate antigens (CFAs) (37), LPS (38), or crude extracts (38,39) from B. pseudomallei. Similarly, CFA-reactive serum IgM was detected in 66% of septicemic melioidosis patients (37).…”

Section: Figmentioning

confidence: 99%

A Burkholderia pseudomallei Outer Membrane Vesicle Vaccine Provides Protection against Lethal Sepsis

Nieves

Petersen

Judy

et al. 2014

Clin. Vaccine Immunol.

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The environmental Gram-negative encapsulated bacillus Burkholderia pseudomallei is the causative agent of melioidosis, a disease associated with high morbidity and mortality rates in areas of Southeast Asia and northern Australia in which the disease is endemic. B. pseudomallei is also classified as a tier I select agent due to the high level of lethality of the bacterium and its innate resistance to antibiotics, as well as the lack of an effective vaccine. Gram-negative bacteria, including B. pseudomallei, secrete outer membrane vesicles (OMVs) which are enriched with multiple protein, lipid, and polysaccharide antigens. Previously, we demonstrated that immunization with multivalent B. pseudomallei-derived OMVs protects highly susceptible BALB/c mice against an otherwise lethal aerosol challenge. In this work, we evaluated the protective efficacy of OMV immunization against intraperitoneal challenge with a heterologous strain because systemic infection with phenotypically diverse environmental B. pseudomallei strains poses another hazard and a challenge to vaccine development. We demonstrated that B. pseudomallei OMVs derived from strain 1026b afforded significant protection against septicemic infection with B. pseudomallei strain K96243. OMV immunization induced robust OMV-, lipopolysaccharide-, and capsular polysaccharide-specific serum IgG (IgG1, IgG2a, and IgG3) and IgM antibody responses. OMV-immune serum promoted bacterial killing in vitro, and passive transfer of B. pseudomallei OMV immune sera protected naive mice against a subsequent challenge. These results indicate that OMV immunization provides antibody-mediated protection against acute, rapidly lethal sepsis in mice. B. pseudomallei-derived OMVs may represent an efficacious multivalent vaccine strategy against melioidosis.

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Section: Figmentioning

confidence: 99%

A Burkholderia pseudomallei Outer Membrane Vesicle Vaccine Provides Protection against Lethal Sepsis

Nieves

Petersen

Judy

et al. 2014

Clin. Vaccine Immunol.

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“…These occupations involve exposure to soil, dust, and wind, increasing exposure to the environmental B. pseudomallei. In Brazil, there is a significant association between construction workers and B. pseudomallei seropositivity, 13 which may be caused by the liberation of organisms into the air during soil excavation. 14 The majority of the patients presented with pneumonia (44.0%) and fever (41.7%) followed by skin or soft tissue infections (27.4%).…”

Section: Fastdigest Greenmentioning

confidence: 99%

Predictors of Severe Disease in Melioidosis Patients in Kuala Lumpur, Malaysia

Roslani

Tay

Puthucheary

et al. 2014

The American Journal of Tropical Medicine and Hygiene

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Abstract. The predictors of severe disease or death were determined for 85 melioidosis patients in Kuala Lumpur, Malaysia. Most of the patients were male, 40 years old, and diabetic. Severe disease or death occurred in 28 (32.9%) cases. Lower lymphocyte counts and positive blood cultures were significant independent predictors of severe disease, but age, presentations with pneumonia, inappropriate empirical antibiotics, or flagellin types of the infecting isolates were not. Knowledge of local predictors of severe disease is useful for clinical management.Melioidosis is endemic in southeast Asia and northern Australia. Clinical manifestations are diverse, with the most severe forms often leading to death. Mortality rates vary widely between settings (for example, between Australian [19%] and Thai [50%] patients).1 This may be because of differences in patient populations, strain virulence, environmental factors, and healthcare facilities. It is not certain whether variations within virulence coding genes influence severity of melioidosis. The flagellin protein in Burkholderia pseudomallei is involved in mobility, invasion, and virulence.2 The flagellin (fliC) gene has been used to type B. pseudomallei by polymerase chain reaction (PCR) -restriction fragment-length polymorphism (RFLP).3 However, flagellin types have yet to be correlated with clinical presentation. It is important to predict which patients are most at risk of severe disease to institute earlier interventions, such as appropriate antibiotics and intensive care unit (ICU) admission. Therefore, the objectives of this study were to determine clinical and laboratory predictors of severe melioidosis, including flagellin types.This retrospective study reviewed clinical and laboratory data of patients with a first presentation of culture-positive melioidosis from any site from August of 1988 to June of 2010 at a teaching hospital in Kuala Lumpur, Malaysia. A standard data collection form was used. Severe disease was defined as death or requirement of ICU admission, ventilation, or inotropic support. Appropriate empirical antibiotics were defined as treatment started before culture results were available using any of the antibiotics recommended for melioidosis treatment, which are ceftazidime, imipenem, meropenem, coamoxiclav, doxycycline, chloramphenicol, and cotrimoxazole. 4 Approval was obtained from the hospital's Medical Ethics Committee (reference no. 733.8).Flagellin gene typing was carried out on available stocked isolates of B. pseudomallei as described in an earlier study, 3 which included isolates of 24 patients included in this study. Bacterial isolates were recovered from stocks on MuellerHinton or blood agar at 37 C for 24-48 hours. Colonies were suspended in 500 μL sterile distilled water, heated at 100 C for 30 minutes, cooled down in ice, briefly vortexed, and centrifuged at 12,000 rpm for 1 minute. The supernatant was used as the DNA template for amplification of the fliC gene using the primer pairs BC6E (5 -ACCAACAGCCTGCAGCGTATC-3 ) and ...

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“…Phylogenetic analysis of different B. pseudomallei isolates, in the world, point out to the soil as their origin, as commonly reported in tropical and subtropical regions as the Southeast Asia, Australia and India (Radua et al, 2000;Nandagopal et al, 2012). In Brazil, few sporadic cases of B. pseudomallei detection, infection and related diseases have been reported (Miralles et al, 2004;Rolim et al, 2011). Here, it is described the first case of B. pseudomallei isolation from soil collected in the Amapá state, in northern Brazil, but unnoticed infection or related disease in man or animals etiologically linked to this microorganism is unknown and of great concern to health authorities due to disease severity and the high mortality rate.…”

Section: Introductionmentioning

confidence: 93%

Preliminary Molecular Studies of the First Report of Burkholderia pseudomallei Isolation from Soil Collected in the Amapá State, in Northern Brazil

Sousa¹,

Segovia

Martins³

et al. 2015

IJB

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The wide spectra of colonizing microorganism, likewise Burkholderia pseudomallei, has been found in different habitats, and presenting distinct activity, as exerting physiological functions among plants, or as a pathogen for man, animals and also as a phytopathogen. A common disease of men and animals caused by B. pseudomallei, melioidosis, is a severe morbidity that usually culminates in the host death. Soil samples from different areas in the Amapá state, in northern Brazil, were screened for environmental microorganisms to assess potential antimicrobial activity aiming at biotechnological applications. Among the prospected microorganisms, B. pseudomallei was isolated from high humidity soils, mangrove, which is rich in organic materials, produced by the diversified local flora and fauna. The isolated B. pseudomallei was identified by its biochemical profile and growth characteristics. Molecular confirmation of B. pseudomallei phenotypic identification was achieved by PCR amplification of the 16 S ribosomal DNA. The sequencing of amplified products confirmed that the Amapá sample, and two other isolates from human infections in Ceará state, northeast Brazil, were B. pseudomallei, and sequence alignement to the same specie, MSHR146 strain from Australia, and clone YN01 from uncultured Burkholderia sp., deposited in the GenBank, exhibited close phylogenetic relationship among them. Until now, there is no report of B. pseudomallei related disease among human and animal populations in the Amapá state, despite the finding of B. pseudomallei in it, in an area of water buffalo ranching and flowing small rivers utilized by human populations.

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Burkholderia pseudomallei Antibodies in Individuals Living in Endemic Regions in Northeastern Brazil

Cited by 16 publications

References 19 publications

A Burkholderia pseudomallei Outer Membrane Vesicle Vaccine Provides Protection against Lethal Sepsis

A Burkholderia pseudomallei Outer Membrane Vesicle Vaccine Provides Protection against Lethal Sepsis

Predictors of Severe Disease in Melioidosis Patients in Kuala Lumpur, Malaysia

Preliminary Molecular Studies of the First Report of Burkholderia pseudomallei Isolation from Soil Collected in the Amapá State, in Northern Brazil

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