Burkholderia megalochromosomata sp. nov., isolated from grassland soil

Abstract

A Gram-stain negative, rod-shaped, non-spore-forming, obligate aerobic bacterial strain, JC2949 T , was isolated from grassland soil in Gwanak Mountain, Seoul, Republic of Korea. Phylogenetic analysis, based on 16S rRNA sequences, indicated that strain JC2949 T belongs to the genus Burkholderia, showing highest sequence similarities with Burkholderia grimmiae R27 T (98.8 %), Burkholderia cordobensis LMG 27620 T (98.6 %), Burkholderia jiangsuensis MP-1T T (98.6 %), Burkholderia zhejiangensis OP-1 T (98.5 %), Burkholderia humi LMG 22934 T (97.5 %), Burkholderia terrestris LMG 22937 T (97.3 %), Burkholderia telluris LMG 22936 T (97.2 %) and Burkholderia glathei ATCC 29195 T (97.0 %). The major fatty acids of strain JC2949 T were C 18 : 1 v7c, summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c) and C 16 : 0 . Its predominant polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown amino phospholipid. The dominant isoprenoid quinone was ubiquinone Q-8. The pairwise average nucleotide identity values between strain JC2949 T and the genomes of 30 other species of the genus Burkholderia ranged from 73.4-90.4 %, indicating that the isolate is a novel genomic species within this genus. Based on phenotypic and chemotaxonomic comparisons, it is clear that strain JC2949 T represents a novel species of the genus Burkholderia. We propose the name for this novel species to be Burkholderia megalochromosomata sp. nov. The type strain is JC2949 T (5KACC 17925 T 5JCM 19905 T ).

Identification and characterization of Burkholderia multivorans CCA53

“…This suggests that ubiquinone-8 exclusively functions in the quinone system of B. multivorans CCA53, which is consistent with the quinone profiles of B. kururiensis [29], B. megalochromosomata [30] and B. uboniae [31]. …”

Phylogenomic Study of Burkholderia glathei-like Organisms, Proposal of 13 Novel Burkholderia Species and Emended Descriptions of Burkholderia sordidicola, Burkholderia zhejiangensis, and Burkholderia grimmiae

“…Table 1 lists the sources of the 17 studied isolates. Details of type strains of each of the present BGC species were described previously (Zolg and Ottow, 1975 ; Lim et al, 2003 ; Lu et al, 2012 ; Tian et al, 2013 ; Vandamme et al, 2013 ; Draghi et al, 2014 ; Liu et al, 2014 ; Baek et al, 2015 ). Strains were grown aerobically on buffered nutrient agar (Oxoid, pH 6.8) and incubated at 28°C.…”

“… a Status complete: RPE64, RPE67, YI23; status draft assembly: all other genomes . b Genome sequence not publicly available, contig sequences were provided by J. Chun (Baek et al, 2015 ) . …”

“… b Genome sequence not publicly available, contig sequences were provided by J. Chun (Baek et al, 2015 ) . …”

“…Although this clade thus far includes only 12 formally named species, its functional diversity is impressive. In this clade too, most species have been isolated from bulk and rhizosphere soil (Zolg and Ottow, 1975 ; Viallard et al, 1998 ; Vandamme et al, 2013 ; Draghi et al, 2014 ; Baek et al, 2015 ), but also from contaminated soil and sludge from a wastewater treatment system (Lu et al, 2012 ; Vandamme et al, 2013 ; Liu et al, 2014 ). Two BGC species were associated with less studied hosts like fungi ( Burkholderia sordidicola ) and mosses ( Burkholderia grimmiae ) (Lim et al, 2003 ; Tian et al, 2013 ) but numerous, mostly uncultivated BGC species adopted endosymbiotic lifestyles in insect guts (Kikuchi et al, 2011 ; Tago et al, 2015 ; Xu et al, 2016 ) or plant leaf tissue (Verstraete et al, 2013 ; Carlier et al, 2015 ) and many additional unclassified B. glathei -like bacteria have been reported (Nogales et al, 2001 ; Salles et al, 2006 ; Pumphrey and Madsen, 2008 ; Draghi et al, 2014 ; Verstraete et al, 2014 ; Peeters et al, 2016 ).…”